LOCUS       V028863                10627 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V028863
VERSION     V028863
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             234..414
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /label="5' LTR (truncated)"
     misc_feature    458..583
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    1076..1309
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             1493..1537
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
     misc_feature    1804..1921
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     promoter        2011..2214
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="CMV promoter"
     CDS             2317..4653
                     /label="Acad11(NM_175324)"
                     /note="Acad11(NM_175324)"
                     /gene="Acad11"
     CDS             4660..4725
                     /codon_start=1
                     /product="three tandem FLAG® epitope tags, followed by an
                     enterokinase cleavage site"
                     /transl_table=1
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
                     /label="3xFLAG"
     promoter        4769..4980
                     /note="core&nbsp;promoter for human elongation factor
                     EF-1α"
                     /label="EF-1α core promoter"
     LTR             4993..5261
                     /note="truncated 5' long terminal repeat (LTR) from human
                     T-cell leukemia virus (HTLV) type 1"
                     /label="5' LTR (truncated)"
     regulatory      5290..5299
                     /note="vertebrate consensus sequence for strong initiation
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
                     /label="Kozak sequence"
     CDS             5320..5982
                     /codon_start=1
                     /product="green fluorescent protein 2 from Pontellina
                     plumata, also known as ppluGFP2 (Shagin et al., 2004)"
                     /transl_table=1
                     /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT
                     FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY
                     EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG
                     YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA"
                     /label="CopGFP"
     CDS             6052..6105
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from&nbsp;Thosea asigna virus capsid
                     protein"
                     /protein_id=""
                     /transl_table=1
                     /translation="EGRGSLLTCGDVEENPGP"
                     /label="T2A"
     CDS             6106..6705
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    6706..7294
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             7368..7601
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     polyA_signal    7673..7794
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     rep_origin      7834..7969
                     /note="SV40 origin of replication"
                     /label="SV40 ori"
     primer_bind     complement(8002..8018)
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 rev"
     protein_bind    8026..8042
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(8050..8080)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    8095..8116
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(8404..8992)
                     /direction=LEFT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     CDS             complement(9163..10023)
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     promoter        complement(10024..10128)
                     /gene="bla"
                     /label="AmpR promoter"
     primer_bind     10602..10618
                     /note="common sequencing primer, one of multiple similar
                     variants"
                     /label="M13 fwd"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
      241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
      301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
      361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
      421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
      481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
      541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
      601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
      661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
      721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
      781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
      841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
      901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg
      961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
     1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
     1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
     1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
     1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
     1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
     1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
     1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcggtt
     1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat
     1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt
     1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggttt atataagcag agctcgttta
     2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc
     2281 tagagctagc ctgatacgaa ctcggaattc gccaccatgg agacggatgt caccagtgac
     2341 accgtggaag tgctacccca gcacaagttc gacctcaggt ccctggaggc ctacctgaat
     2401 cagcacttgc cgggctttgg gtcagattcc cgggctgtgc tgactgttac tcaatacaga
     2461 tcaggacagt caaacccaac cttctttctc cagaaaggat ctcaagccta tgttctcagg
     2521 aagaaaccac ccggctctct tcttcccaaa gcacataaga ttgacagaga attcaagatc
     2581 cagaaagcct tgttttccat tggatttcct gttgccaagc ccctgctata ctgcagagat
     2641 gcttcagtta tcggaacgga attttatgta atggaacatg tgcagggtcg gatctttcgt
     2701 gatttttcca ttcctggagt tagctcagca gaacgtgctg ctatatatgt gtctgtggca
     2761 gaaaccctgg catggctaca ttctctggat atacgctcgc tgaagctgga caaatacggt
     2821 actggagtcg ggtactgcaa aaggcaggtg tccacctgga cgaagcagta ccaggcctca
     2881 gcccatcaga gcatccctgc catggaccag ctctccacgt ggctgatgaa gaacttgcca
     2941 gatagtgaca gtgaggagtg cctggtccac ggagacttca agctggacaa catagttttc
     3001 caccccaaag agtgtcgggt tatagcagtg ttggactggg agctctcgac cttcggccat
     3061 cccttgacgg acttagccca tctttccttg ttttactatt ggcccaggac actgcccatg
     3121 ataaaccgag gctctcacat tccagagaac acaggaatac cgttgatgga agaactgatt
     3181 tcaatatact gccaccgaag gggaattgat cctaatctcc ctaactggaa tttctttatg
     3241 gccctctcat tttttaaatt ggctggaata tcacagggag tctatcgtag atacctcatg
     3301 ggaaataact catctgagga tagcttctta actgccaata ctgtgcaacc tctggcagaa
     3361 actggattgc agctgtcaaa aagaactctc aggactacac caccacaggc tgatgctaaa
     3421 agccagctgt ttgcacagag caggagaggc caggaagttc tcactagagt gaagcagttc
     3481 atgaagcagc atgtctttcc tgctgagaag gaagtagctg aatactatgc tcaaagtgga
     3541 aactcagcgg aaaagtgggg acacccctta gtgatcgaaa aactaaagga aatagccaaa
     3601 gcagagggcc tgtggaattt gttcttgcca gctgtgagcg gtctcagcca ggtggactat
     3661 gccttgattg ctgaagagac aggaaaatgc ttttttgctc cagatgtttt taactgccaa
     3721 gcaccagaca caggcaacat ggaggtactg cacctgtatg gcagcgagca gcagaagaag
     3781 cagtggctgg agcctctcct gcgtggcgat atcacctccg tcttctgtat gacagagccc
     3841 aacgtctcat caagcgatgc cacgaacatc gaatgcacca tccagagaga tggaggtggc
     3901 tatatagtta acggcaagaa gtggtggagc agcggagctg ggaatcccaa gtgcaaaatc
     3961 gccattgttt tgggaagaac ggagtctccc tctgcatcca gacacagaca gcacagcatg
     4021 atcctcgtgc cgatggacac acctggagtg gagttaataa ggcctttgtc ggtgttcggc
     4081 tacatggata atatgcatgg cgggcactgg gaggtccatt tcaatcacgt gcgcgttcct
     4141 gccagcaact taatactagg tgaaggcagg ggctttgaaa tttcccaagg ccgccttgga
     4201 cctggaagaa tccaccactg tatgagaacc gtgggtttgg cagaacgcat tctgcagatc
     4261 atgtgtgacc gggccgtgca gagggaggcc ttcaagaaga agctctacga acatgaagtc
     4321 gtggctcact ggattgctaa gagccgtata gccatcgagg agatccgctt gctgaccttg
     4381 aaagcggctc acagcataga tactctgggc agtgcctcag ccaggaagga gattgcaatg
     4441 atcaaagtgg ctgcccccaa agctgtctgc aaaatagctg actgggccat ccaggtgcac
     4501 ggaggcgcgg gagtttccca ggattaccct ctggctaaca tgtacgccat catacgcacc
     4561 ctgcgcttgg cagacggccc tgacgaagtc cacctctcag cgatcgccaa gatggagctt
     4621 caggaccaag ccagacggtt gacagccagg atgggatccg actacaaaga ccatgacggt
     4681 gattataaag atcatgacat cgactacaag gatgacgatg acaagtagcg gccgcgaagg
     4741 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4801 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac
     4861 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     4921 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     4981 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc
     5041 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg
     5101 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct
     5161 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc
     5221 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct
     5281 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc
     5341 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc
     5401 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc
     5461 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc
     5521 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc
     5581 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc
     5641 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc
     5701 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac
     5761 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc
     5821 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg
     5881 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc
     5941 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg
     6001 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga
     6061 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag
     6121 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     6181 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     6241 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     6301 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     6361 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     6421 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     6481 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     6541 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     6601 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     6661 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat
     6721 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     6781 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     6841 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     6901 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     6961 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     7021 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     7081 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     7141 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     7201 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     7261 acgagtcgga tctccctttg ggccgcctcc ccgcctggta cctttaagac caatgactta
     7321 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     7381 tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct ggttagacca
     7441 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     7501 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     7561 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     7621 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     7681 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     7741 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     7801 ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     7861 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     7921 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc
     7981 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac
     8041 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     8101 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     8161 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     8221 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     8281 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     8341 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     8401 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     8461 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     8521 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     8581 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     8641 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     8701 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     8761 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     8821 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     8881 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     8941 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     9001 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     9061 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     9121 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     9181 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     9241 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     9301 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     9361 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     9421 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     9481 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     9541 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     9601 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     9661 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     9721 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     9781 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     9841 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     9901 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     9961 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
    10021 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
    10081 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    10141 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag
    10201 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca
    10261 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
    10321 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc
    10381 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag
    10441 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg
    10501 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg
    10561 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc
    10621 caagctg
//
