LOCUS V040762 11048 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V040762 VERSION V040762 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers polyA_signal 216..350 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" promoter 398..502 /gene="bla" /label="AmpR promoter" CDS 503..1363 /codon_start=1 /gene="bla" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /product="β-lactamase" /transl_table=1 /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA SLIKHW*" /label="AmpR" rep_origin 1534..2119 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" protein_bind 2407..2428 /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." /label="CAP binding site" promoter 2443..2473 /note="promoter for the E. coli lac operon" /label="lac promoter" protein_bind 2481..2497 /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside (IPTG)." /label="lac operator" primer_bind 2505..2521 /note="common sequencing primer, one of multiple similar variants" /label="M13 rev" LTR 2650..3283 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" misc_feature 3490..4078 /note="woodchuck hepatitis virus posttranscriptional regulatory element" /label="WPRE" CDS complement(4092..4691) /codon_start=1 /gene="pac from Streptomyces alboniger" /note="confers resistance to puromycin" /product="puromycin N-acetyltransferase" /transl_table=1 /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*" /label="PuroR" CDS complement(4701..4754) /codon_start=1 /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /product="2A peptide from Thosea asigna virus capsid protein" /protein_id="" /transl_table=1 /translation="EGRGSLLTCGDVEENPGP" /label="T2A" CDS complement(4824..5486) /codon_start=1 /product="green fluorescent protein 2 from Pontellina plumata, also known as ppluGFP2 (Shagin et al., 2004)" /transl_table=1 /translation="PAMEIECRITGTLNGVEFELVGGGEGTPKQGRMTNKMKSTKGALT FSPYLLSHVMGYGFYHFGTYPSGYENPFLHAINNGGYTNTRIEKYEDGGVLHVSFSYRY EAGRVIGDFKVVGTGFPEDSVIFTDKIIRSNATVEHLHPMGDNVLVGSFARTFSLRDGG YYSFVVDSHMHFKSAIHPSILQNGGPMFAFRRVEELHSNTELGIVEYQHAFKTPIAFA" /label="CopGFP" regulatory 5507..5516 /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" /regulatory_class="other" /label="Kozak sequence" promoter complement(5540..5869) /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS complement(5880..6626) /codon_start=1 /product="modified rtTA protein that binds tightly to promoters containing the tet operator in the presence of doxycycline" /transl_table=1 /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA DALDDFDLDMLPADALDDFDLDMLPG*" /label="Tet-On® 3G" promoter complement(6645..7155) /note="human phosphoglycerate kinase 1 promoter" /label="hPGK promoter" promoter 7172..7539 /note="3rd-generation Tet-responsive promoter that can be activated by binding of Tet-On® 3G, modified to eliminate binding sites for endogenous mammalian transcription factors" /label="TRE3GS promoter" CDS 7552..8478 /label="Ucp2(NM_011671)" /note="Ucp2(NM_011671)" /gene="Ucp2" CDS 8485..8550 /codon_start=1 /product="three tandem FLAG® epitope tags, followed by an enterokinase cleavage site" /transl_table=1 /translation="DYKDHDGDYKDHDIDYKDDDDK" /label="3xFLAG" polyA_signal 8728..8862 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" misc_feature 8906..9021 /note="central polypurine tract and central termination sequence of HIV-1 (lacking the first T)" /label="cPPT/CTS" CDS complement(9284..9328) /codon_start=1 /note="recognized by the 2H10 single-chain llama nanobody" /product="antigenic peptide corresponding to amino acids 655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik et al., 2013)" /transl_table=1 /translation="KNEQELLELDKWASL" /label="gp41 peptide" misc_feature 9513..9746 /note="The Rev response element (RRE) of HIV-1 allows for Rev-dependent mRNA export from the nucleus to the cytoplasm." /label="RRE" misc_feature 10243..10368 /note="packaging signal of human immunodeficiency virus type 1" /label="HIV-1 Ψ" LTR 10415..11048 /note="3' long terminal repeat (LTR) from HIV-1" /label="3' LTR" ORIGIN 1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg 61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg 121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt 181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg 241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt 301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga 361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct 721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat 781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa 1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc 1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat 1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct 1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct 1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat 2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc 2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc 2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc 2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga 2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc 2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg 2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac 2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag 2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc 2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac 2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag 2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga 3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca 3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta 3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct 3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga 3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt 3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt 3361 gtgactggaa aacccacctc ctcctcctct tgtgcttcta gccaggcaca atcagcattg 3421 gtagctgctg tattgctact tgtgattgct ccatgttttt ctaggtctcg actgcagaat 3481 taattccagg cggggaggcg gcccaaaggg agatccgact cgtctgaggg cgaaggcgaa 3541 gacgcggaag aggccgcaga gccggcagca ggccgcggga aggaaggtcc gctggattga 3601 gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat ccaggtggca acacaggcga 3661 gcagccatgg aaaggacgtc agcttccccg acaacaccac ggaattgtca gtgcccaaca 3721 gccgagcccc tgtccagcag cgggcaaggc aggcggcgat gagttccgcc gtggcaatag 3781 ggagggggaa agcgaaagtc ccggaaagga gctgacaggt ggtggcaatg ccccaaccag 3841 tgggggttgc gtcagcaaac acagtgcaca ccacgccacg ttgcctgaca acgggccaca 3901 actcctcata aagagacagc aaccaggatt tatacaagga ggagaaaatg aaagccatac 3961 gggaagcaat agcatgatac aaaggcatta aagcagcgta tccacatagc gtaaaaggag 4021 caacatagtt aagaatacca gtcaatcttt cacaaatttt gtaatccaga ggttgattgt 4081 tccagacgcg ttcaggcacc gggcttgcgg gtcatgcacc aggtgcgcgg tccttcgggc 4141 acctcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag gttgcggggc 4201 gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac tccggggagc 4261 acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac ggtggccagg 4321 aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg ttgctgcgcg 4381 gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa caccgccccc 4441 gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc cgcgacccac 4501 accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc ggtgacccgc 4561 tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc gaacgcggcg 4621 gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac cgtgggcttg 4681 tactcggtca tggtcgcgtc agggccggga ttctcctcca cgtcaccgca tgttagaaga 4741 cttcctctgc cctcgcgaga tccggtggag ccgggtccgg cggtgccgtc cacggcagaa 4801 ttggacgact gagcgcggga tctggcgaag gcgatggggg tcttgaaggc gtgctggtac 4861 tccacgatgc ccagctcggt gttgctgtgc agctcctcca cgcggcggaa ggcgaacatg 4921 gggcccccgt tctgcaggat gctggggtgg atggcgctct tgaagtgcat gtggctgtcc 4981 accacgaagc tgtagtagcc gccgtcgcgc aggctgaagg tgcgggcgaa gctgcccacc 5041 agcacgttat cgcccatggg gtgcaggtgc tccacggtgg cgttgctgcg gatgatcttg 5101 tcggtgaaga tcacgctgtc ctcggggaag ccggtgccca ccaccttgaa gtcgccgatc 5161 acgcggccgg cctcgtagcg gtagctgaag ctcacgtgca gcacgccgcc gtcctcgtac 5221 ttctcgatgc gggtgttggt gtagccgccg ttgttgatgg cgtgcaggaa ggggttctcg 5281 tagccgctgg ggtaggtgcc gaagtggtag aagccgtagc ccatcacgtg gctcagcagg 5341 taggggctga aggtcagggc gcctttggtg ctcttcatct tgttggtcat gcggccctgc 5401 ttgggggtgc cctctccgcc gcccaccagc tcgaactcca cgccgttcag ggtgccggtg 5461 atgcggcact cgatctccat ggcgggcagg ccgctctcgt cgctctccat ggtggcgtct 5521 agcgtaggcg ccggtcacat ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact 5581 tctggaatag ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc 5641 agccatgggg cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta 5701 ggggcgggac tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg 5761 gggagcctgg ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac 5821 ttctgcctgc tggggagcct ggggactttc cacaccctaa ctgacacaca ttcgcgcgtt 5881 tacccgggga gcatgtcaag gtcaaaatcg tcaagagcgt cagcaggcag catatcaagg 5941 tcaaagtcgt caagggcatc ggctgggagc atgtctaagt caaaatcgtc aagggcgtcg 6001 gtcggcccgc cgctttcgca ctttagctgt ttctccaggc cacatatgat tagttccagg 6061 ccgaaaagga aggcaggttc ggctccctgc cggtcgaaca gctcaattgc ttgtttcaga 6121 agtgggggca tagaatcggt ggtaggtgtc tctctttcct cttttgctac ttgatgctcc 6181 tgttcctcca atacgcagcc cagtgtaaag tggcccacgg cggacagagc gtacagtgcg 6241 ttctccaggg agaagccttg ctgacacagg aacgcgagct gattttccag ggtttcgtac 6301 tgtttctctg ttgggcgggt gccgagatgc actttagccc cgtcgcgatg tgagaggaga 6361 gcacagcggt atgacttggc gttgttccgc agaaagtctt gccatgactc gccttccagg 6421 gggcaggagt gggtatgatg cctgtccagc atctcgattg gcagggcatc gagcagggcc 6481 cgcttgttct tcacgtgcca gtacagggta ggctgctcaa ctcccagctt ttgagcgagt 6541 ttccttgtcg tcaggccttc gataccgact ccattgagta attccagagc agagtttatg 6601 actttgctct tgtccagtct agacatggta attcgatgat ccccctgggg agagaggtcg 6661 gtgattcggt caacgaggga gccgactgcc gacgtgcgct ccggaggctt gcagaatgcg 6721 gaacaccgcg cgggcaggaa cagggcccac actaccgccc cacaccccgc ctcccgcacc 6781 gccccttccc ggccgctgct ctcggcgcgc cctgctgagc agccgctatt ggccacagcc 6841 catcgcggtc ggcgcgctgc cattgctccc tggcgctgtc cgtctgcgag ggtactagtg 6901 agacgtgcgg cttccgtttg tcacgtccgg cacgccgcga accgcaagga accttcccga 6961 cttaggggcg gagcaggaag cgtcgccggg gggcccacaa gggtagcggc gaagatccgg 7021 gtgacgctgc gaacggacgt gaagaatgtg cgagacccag ggtcggcgcc gctgcgtttc 7081 ccggaaccac gcccagagca gccgcgtccc tgcgcaaacc cagggctgcc ttggaaaagg 7141 cgcaacccca accccgtgga attatcacct cgagtttact ccctatcagt gatagagaac 7201 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca 7261 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt 7321 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac 7381 gtatatccag tttactccct atcagtgata gagaacgtat aagctttgct tatgtaaacc 7441 agggcgccta taaaagagtg ctgatttttt gagtaaactt caattccaca acacttttgt 7501 cttataccaa ctttccgtac cacttcctac cctcgtaaag aattcgccac catggttggt 7561 ttcaaggcca cagatgtgcc cccaacagcc actgtgaagt tcctgggggc tgggacagct 7621 gcctgcattg cagatctcat cactttccct ctggataccg ccaaggtccg gctgcagatc 7681 caaggggaga gtcaagggct agtgcgcacc gcagccagcg cccagtaccg tggcgttctg 7741 ggtaccatcc taaccatggt gcgcactgag ggtccacgca gcctctacaa tgggctggtc 7801 gccggcctgc agcgccagat gagctttgcc tccgtccgca ttggcctcta cgactctgtc 7861 aaacagttct acaccaaggg ctcagagcat gcaggcatcg ggagccgcct cctggcaggt 7921 agcaccacag gtgccctggc cgtggctgta gcccagccta cagatgtggt aaaggtccgc 7981 ttccaggctc aggcccgggc tggtggtggt cggagatacc agagcactgt cgaagcctac 8041 aagaccattg cacgagagga agggatccgg ggcctctgga aagggacttc tcccaatgtt 8101 gcccgtaatg ccattgtcaa ctgtgctgag ctggtgacct atgacctcat caaagatact 8161 ctcctgaaag ccaacctcat gacagatgac ctcccttgcc acttcacttc tgccttcggg 8221 gccggcttct gcaccaccgt catcgcctcc cctgttgatg tggtcaagac gagatacatg 8281 aactctgcct tgggccagta ccacagcgca ggtcactgtg cccttaccat gctccggaag 8341 gagggacccc gcgccttcta caaggggttc atgccttcct ttctccgctt gggatcctgg 8401 aacgtagtga tgtttgtcac ctatgagcag ctcaaaagag ccctaatggc tgcctaccaa 8461 tctcgggagg cacctttcgg atccgactac aaagaccatg acggtgatta taaagatcat 8521 gacatcgact acaaggatga cgatgacaag tagagaccac ctcccctgcg agctaagctg 8581 gacagccaat gacgggtaag agagtgacat ttttcactaa cctaagacag gagggccgtc 8641 agagctactg cctaatccaa agacgggtaa aagtgataaa aatgtatcac tccaacctaa 8701 gacaggcgca gcttccgagg gatttgagat ccagacatga taagatacat tgatgagttt 8761 ggacaaacca aaactagaat gcagtgaaaa aaatgcctta tttgtgaaat ttgtgatgct 8821 attgccttat ttgtaaccat tataagctgc aataaacaag ttaagtcgat aaactggatc 8881 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtagttc 8941 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc 9001 aatcccccct tttcttttaa aggcgatacc gtcgagatcc gttcactaat cgaatggatc 9061 tgtctctgtc tctctctcca ccttcttctt ctattccttc gggcctgtcg ggtcccctcg 9121 gggttgggag gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta 9181 tagaaagtac agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata 9241 attttatata ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat 9301 ctaattccaa taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg 9361 agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat 9421 tccaaatctg ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc 9481 aaatgagttt tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt 9541 ccacagccag gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat 9601 gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata 9661 attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc 9721 ccaagaaccc aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc 9781 ttctctttgc cttggtgggt gctactccta atggttcaat ttttactact ttatatttat 9841 ataattcact tctccaattg tccctcatat ctcctcctcc aggtctgaag atcagcggcc 9901 ggccgcttgc tgtgcggtgg tcttactttt gttttgctct tcctctatct tgtctaaagc 9961 ttccttggtg tcttttatct ctatcctttg atgcacacaa tagagggttg ctactgtatt 10021 atataatgat ctaagttctt ctgatcctgt ctgaagggat ggttgtagct gtcccagtat 10081 ttgtctacag ccttctgatg tttctaacag gccaggatta actgcgaatc gttctagctc 10141 cctgcttgcc catactatat gttttaattt atattttttc tttccccctg gccttaaccg 10201 aattttttcc catcgcgatc taattctccc ccgcttaata ctgacgctct cgcacccatc 10261 tctctccttc tagcctccgc tagtcaaaat ttttggcgta ctcaccagtc gccgcccctc 10321 gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt cgagagagct cctctggttt 10381 ccctttcgct ttcaagtccc tgttcgggcg ccactgctag agattttcca cactgactaa 10441 aagggtctga gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga 10501 agcactcaag gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc 10561 tcccaggctc agatctggtc taaccagaga gacccagtac aggcaaaaag cagctgctta 10621 tatgcaggat ctgagggctc gccactcccc agtcccgccc aggccacgcc tccctggaaa 10681 gtccccagcg gaaagtccct tgtagcaagc tcgatatcag cagttcttga agtactccgg 10741 atgcagctct cgggccacgt gatgaaatgc taggcggctg tcaaacctcc actctaacac 10801 ttctctctcc gggtcatcca tcccatgcag gctcacaggg tgtaacaagc tggtgttctc 10861 tcctttattg gcctcttcta ccttatctgg ctcaactggt actagcttgt agcaccatcc 10921 aaaggtcagt ggatatctga cccctggccc tggtgtgtag ttctgctaat cagggaagta 10981 gccttgtgtg tggtagatcc acagatcaag gatatcttgt cttctttggg agtgaattag 11041 cccttcca //