LOCUS       Exported               10937 bp DNA     circular SYN 30-JUN-2026
DEFINITION  synthetic circular DNA.
ACCESSION   V042410
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10937)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1 (lacking the first T)"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2815..4902
                     /codon_start=1
                     /gene="GOLIM4"
                     /label=GOLIM4(NM_014498)
                     /note="GOLIM4(NM_014498)"
                     /translation="MGNGMCSRKQKRIFQTLLLLTVVFGFLYGAMLYYELQTQLRKAEA
                     VALKYQQHQESLSAQLQVVYEHRSRLEKSLQKERLEHKKAKEDFLVYKLEAQETLNKGR
                     QDSNSRYSALNVQHQMLKSQHEELKKQHSDLEEEHRKQGEDFSRTFNDHKQKYLQLQQE
                     KEQELSKLKETVYNLREENRQLRKAHQDIHTQLQDVKQQHKNLLSEHEQLVVTLEDHKS
                     ALAAAQTQVAEYKQLKDTLNRIPSLRKPDPAEQQNVTQVAHSPQGYNTAREKPTREVQE
                     VSRNNDVWQNHEAVPGRAEDTKLYAPTHKEAEFQAPPEPIQQEVERREPEEHQVEEEHR
                     KALEEEEMEQVGQAEHLEEEHDPSPEEQDREWKEQHEQREAANLLEGHARAEVYPSAKP
                     MIKFQSPYEEQLEQQRLAVQQVEEAQQLREHQEALHQQRLQGHLLRQQEQQQQQVAREM
                     ALQRQAELEEGRPQHQEQLRQQAHYDAMDNDIVQGAEDQGIQGEEGAYERDNQHQDEAE
                     GDPGNRHEPREQGPREADPESEADRAAVEDINPADDPNNQGEDEFEEAEQVREENLPDE
                     NEEQKQSNQKQENTEVEEHLVMAGNPDQQEDNVDEQYQEEAEEEVQEDLTEEKKRELEH
                     NAEETYGENDENTDDKNNDGEEQEVRDDNRPKGREEHYEEEEEEEEDGAAVAEKSHRRA
                     EM"
     CDS             4939..5658
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    5687..6273
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6293..6892
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     misc_feature    6906..7494
                     /label=WPRE
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional 
                     regulatory element"
     LTR             7701..8334
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8462..8478)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8486..8502
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8510..8540)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8555..8576
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8864..9452)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(9623..10483)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10484..10588)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    10636..10770
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcgc caccatggga
     2821 aacgggatgt gctcccgaaa gcagaagcgg attttccaga cgctgctgct gctgaccgtc
     2881 gtgttcggct ttctctacgg cgcgatgctc tactacgagc tgcagacgca gctgcggaaa
     2941 gccgaggcgg tggcgctcaa gtaccagcag caccaggagt ccctctccgc ccagttacaa
     3001 gttgtatatg aacacagatc aagattagag aaatccttgc aaaaagaaag acttgaacat
     3061 aaaaaagcaa aggaagattt tcttgtttat aagttagaag cacaagaaac attaaataaa
     3121 ggaaggcaag attccaatag cagatacagt gcactgaatg tccaacatca gatgttgaaa
     3181 agccaacacg aggagctaaa gaaacagcac agtgacttgg aagaggaaca tcgcaaacaa
     3241 ggggaagact tcagtagaac atttaatgac cataagcaaa aatacttgca gctccagcaa
     3301 gaaaaagaac aagaactttc taagctaaaa gagactgtat acaatttgag agaagagaat
     3361 agacaactaa ggaaagcaca ccaagacata catacacagc ttcaagatgt caagcaacag
     3421 cataagaatt tactctccga gcatgaacaa cttgtagtga ctttggaaga ccacaagagt
     3481 gcactagctg ctgcacagac tcaagttgca gaatataaac aactgaaaga tactctgaat
     3541 aggattccaa gccttcgaaa acctgatcca gcagaacagc aaaatgtgac ccaggtggca
     3601 cattctccac aaggttacaa cacagcaagg gagaagccaa cccgagaggt gcaggaggtg
     3661 tctcgaaata atgatgtgtg gcagaaccat gaagcagttc ctggaagagc agaagacaca
     3721 aaactctatg ctcccaccca taaggaggca gaatttcagg ctcccccaga gccaatccaa
     3781 caagaagtgg aacgcagaga acctgaggag catcaggtgg aagaggagca cagaaaggcc
     3841 ctggaggagg aagaaatgga gcaggtcggg caagcagaac atcttgagga ggaacacgat
     3901 ccatcaccag aggagcagga tcgggagtgg aaagagcagc atgagcaacg agaagcagcc
     3961 aacctcctgg aagggcacgc gcgtgctgag gtgtaccctt cagccaagcc aatgatcaaa
     4021 ttccaatcac cctatgagga acagttggaa cagcagagac tggcagtgca gcaggtggag
     4081 gaggcccagc agctgcggga acaccaggaa gctttgcacc agcagaggct gcaggggcac
     4141 ttactacggc agcaggaaca gcagcagcag caggtggcaa gagagatggc cctgcagagg
     4201 caggctgagc ttgaggaggg ccggccgcag caccaggagc agctccggca gcaagctcat
     4261 tatgatgcta tggataatga tatcgttcag ggagcagagg accagggaat ccaaggagag
     4321 gaaggagcct atgaaagaga caaccagcac caagatgaag cagaaggaga tccaggtaat
     4381 agacatgagc ctcgtgaaca aggaccccga gaagccgacc cagaatctga ggcagatagg
     4441 gcagctgtag aagatataaa cccagcagat gaccctaata atcaaggtga ggatgaattt
     4501 gaagaagccg agcaagtgag agaagaaaat ttgccagatg aaaatgaaga gcaaaaacaa
     4561 agtaatcaaa agcaagagaa tacagaagtg gaggaacatt tggtgatggc aggaaatcca
     4621 gaccagcagg aggacaatgt tgatgaacag taccaggaag aggcagaaga ggaggttcag
     4681 gaagatttga ctgaagagaa aaaaagggaa ctggagcata atgctgaaga gacctatggt
     4741 gaaaatgatg aaaatactga tgataaaaat aatgatggag aagagcaaga agttcgagat
     4801 gacaaccgcc ccaaaggccg agaggaacac tacgaggagg aagaagagga ggaagaagac
     4861 ggggctgcag ttgctgagaa atcacatcga agagctgaaa tgctcgaggg cggtggagga
     4921 agcggcggag gtggatctat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     4981 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc
     5041 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     5101 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     5161 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     5221 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     5281 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     5341 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     5401 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac
     5461 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     5521 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     5581 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     5641 gacgagctgt acaagtagac tagttctaga gcggccgcgg atcccgcccc tctccctccc
     5701 ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg tttgtctata
     5761 tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg
     5821 tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg caaggtctgt
     5881 tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca acgtctgtag
     5941 cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc ggccaaaagc
     6001 cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga
     6061 tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg ctgaaggatg
     6121 cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca tgctttacat
     6181 gtgtttagtc gaggttaaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     6241 tttgaaaaac acgatgataa gcttgccaca acccacaagg agacgacctt ccatgaccga
     6301 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc ccccgggccg tacgcaccct
     6361 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgacccgg accgccacat
     6421 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     6481 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     6541 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     6601 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6661 gttcctggcc accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6721 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6781 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6841 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct agacgcgtct
     6901 ggaacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     6961 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7021 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7081 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7141 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7201 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7261 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     7321 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7381 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7441 gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctggaa
     7501 ttaattctgc agtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc
     7561 taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt
     7621 cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     7681 tttaaaagaa aagaggggac tggaagggct aattcactcc caacgaagac aagatatcct
     7741 tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact acacaccagg
     7801 gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac cagttgagcc
     7861 agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc ctgtgagcct
     7921 gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca gccgcctagc
     7981 atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct gatatcgagc
     8041 ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg gcgggactgg
     8101 ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt actgggtctc
     8161 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     8221 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     8281 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta
     8341 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8401 agaggccttg acattgctag cgtttaccgt cgacctctag ctagagcttg gcgtaatcat
     8461 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     8521 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     8581 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     8641 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     8701 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     8761 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     8821 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     8881 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     8941 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9001 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9061 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9121 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg tacctatcgt cttgagtcca
     9181 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9241 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9301 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9361 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9421 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     9481 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     9541 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     9601 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     9661 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     9721 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     9781 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     9841 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     9901 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     9961 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10021 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10081 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10141 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10201 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10261 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10321 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10381 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10441 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    10501 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    10561 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    10621 acggatcggg agatcaactt gtttattgca gcttataatg gttacaaata aagcaatagc
    10681 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
    10741 ctcatcaatg tatcttatca tgtctggatc aactggataa ctcaagctaa ccaaaatcat
    10801 cccaaacttc ccaccccata ccctattacc actgccaatt acctgtggtt tcatttactc
    10861 taaacctgtg attcctctga attattttca ttttaaagaa attgtatttg ttaaatatgt
    10921 actacaaact tagtagt
//
