LOCUS       .                       9202 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2027..2144
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2815..3777
                     /label="BirA(R118G)"
                     /note="promiscuous R118G mutant of the E. coli biotin
                     protein ligase (Cronan, 2005)"
     CDS             3787..3810
                     /label="FLAG"
                     /note="FLAG® epitope tag, followed by an enterokinase
                     cleavage site"
     CDS             3811..3834
                     /label="HRV 3C site"
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     misc_feature    3905..4491
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4511..5107
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     misc_feature    5124..5712
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5919..6552
                     /label="5' LTR"
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6680..6696)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(6704..6720)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6728..6758)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6773..6794)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7082..7670)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(7844..8701)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(8702..8806)
                     /label="AmpR promoter"
     polyA_signal    8854..8988
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
     2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
     2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
     2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
     2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
     2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
     2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
     2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
     2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
     2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
     2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattact cgagatgaag
     2821 gacaacaccg tgcccctgaa gctgatcgcc ctgctggcca acggcgagtt ccacagcggc
     2881 gagcagctgg gcgagaccct gggcatgagc cgggccgcca tcaacaagca catccagacc
     2941 ctgcgggact ggggcgtgga cgtgttcacc gtgcccggca agggctacag cctgcccgag
     3001 cccatccagc tgctgaacgc caagcagatc ctgggccagc tggacggcgg cagcgtggcc
     3061 gtgctgcccg tgatcgacag caccaaccag tacctgctgg accggatcgg cgagctgaag
     3121 agcggcgacg cctgcatcgc cgagtaccag caggccggcc ggggcggccg gggccggaag
     3181 tggttcagcc ccttcggcgc caacctgtac ctgagcatgt tctggcggct ggagcagggc
     3241 cccgccgccg ccatcggcct gagcctggtg atcggcatcg tgatggccga ggtgctgcgg
     3301 aagctgggcg ccgacaaggt gcgggtgaag tggcccaacg acctgtacct gcaggaccgg
     3361 aagctggccg gcatcctggt ggagctgacc ggcaagaccg gcgacgccgc ccagatcgtg
     3421 atcggcgccg gcatcaacat ggccatgcgg cgggtggagg agagcgtggt gaaccagggc
     3481 tggatcaccc tgcaggaggc cggcatcaac ctggaccgga acaccctggc cgccatgctg
     3541 atccgggagc tgcgggccgc cctggagctg ttcgagcagg agggcctggc cccctacctg
     3601 agccggtggg agaagctgga caacttcatc aaccggcccg tgaagctgat catcggcgac
     3661 aaggagatct tcggcatcag ccggggcatc gacaagcagg gcgccctgct gctggagcag
     3721 gacggcatca tcaagccctg gatgggcggc gagatcagcc tgcggagcgc cgagaaggca
     3781 gctatggatt ataaagatga tgatgataaa ctggaagttc tgttccaggg gcccaccggt
     3841 gtcgtcgtcg tcgtcgtcga attcgccaat tcgctggagc agactagagc ggccgcggat
     3901 cccgcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     3961 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4021 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4081 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4141 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4201 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     4261 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     4321 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     4381 ggtgcacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     4441 acggggacgt ggttttcctt tgaaaaacac gatgataagc ttgccacaac ccacaaggag
     4501 acgaccttcc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc
     4561 ccgggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt
     4621 cgacccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt
     4681 cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac
     4741 cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga
     4801 gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg
     4861 gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa
     4921 gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc
     4981 cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac
     5041 cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc
     5101 cggtgcctag acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga
     5161 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt
     5221 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt
     5281 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg
     5341 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg
     5401 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc
     5461 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc
     5521 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct
     5581 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg
     5641 ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg
     5701 ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac
     5761 aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga
     5821 ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc
     5881 tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca
     5941 acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta
     6001 gcagaactac acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa
     6061 gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt
     6121 gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag
     6181 gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa
     6241 gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg
     6301 tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt
     6361 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6421 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6481 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6541 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6601 aatgaatatc agagagtgag aggccttgac attgctagcg tttaccgtcg acctctagct
     6661 agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6721 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6781 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6841 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6901 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6961 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7021 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7081 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7141 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7201 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     7261 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     7321 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     7381 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     7441 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     7501 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     7561 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7621 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7681 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7741 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7801 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7861 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7921 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7981 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8041 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8101 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8161 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     8221 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     8281 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     8341 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     8401 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     8461 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     8521 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     8581 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8641 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8701 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8761 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8821 aagtgccacc tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt
     8881 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8941 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact
     9001 caagctaacc aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac
     9061 ctgtggtttc atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat
     9121 tgtatttgtt aaatatgtac tacaaactta gtagttttta aagaaattgt atttgttaaa
     9181 tatgtactac aaacttagta gt
//
