LOCUS       V033570                12900 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033570
VERSION     V033570
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2356
                     /label="KLHL38(NM_001081675)"
                     /note="KLHL38(NM_001081675)"
                     /gene="KLHL38"
     CDS             2357..2383
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2429..2770
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2896..3395
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3416..4123
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4124..4180
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4181..4780
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4791..5379
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5442..5675
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5700..5718)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6507..6611
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6612..7472
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7643..8231
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8477..8806
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9940..10005
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10135..10155
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10580..10714
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10883..11516
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11563..11688
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12180..12413
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12598..12642
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctactcag aacgcagtga
      601 actccaagcc accatggacg aggagtcact agatgggctg ctcttcaaag accacgactt
      661 ctcttctgac ttgttgaggc agctcaacag cttaaggcaa agcaggatcc tgactgatgt
      721 gagcatctgt gccggtgccc gggagatccc ctgccaccgc aacgtgctgg cctccagcag
      781 cccctacttc agggctatgt tctgcagcag cttccgggag aagagtgaag ccaaagtgca
      841 gctgaaaggc attgaccccc caaccctgga ccagatcgtc tcctacgtgt atacggggga
      901 ggcacatatt gccactgaca atgtcctccc cgtgatggag gccgcctcca tgctacagtt
      961 ccccaagctg tttgaggcct gctcctcgta cttgcagagc cagttggccc ccagcaactg
     1021 cctgggtatg atcagactct cagaaatctt aagctgcgag accctcaaga agaaagccag
     1081 ggaggtggca ctgacgtcct tcccagaggt ggccgcatcg gccgacctga aggagctctg
     1141 tgccttggag ttgagggact atctcggaga tgatgggctc tgtggggagg aggaaaaggt
     1201 gtttgaggcc ctcatggttt ggatcaagca tgacctccag gcccggaagc gatacatgca
     1261 ggaactgttc aagcaggtca ggctgcagta catccaccca gccttctttc accacttcat
     1321 cgccaacgat gccctcctgc agtcctcgcc tgcatgccag atcatcttgg agaccgccaa
     1381 gagacagatg ttctctttgt gtggcaccac cgtcccagac tgcaaactcc tgttgcatgt
     1441 ccctccaaga aactcttacc aagatttcct catcctcttg ggcggaagga aggacagcca
     1501 gcagaccacc agggacgtcc tactgtacag caaacagacc ggccaatggc agagccttgc
     1561 caaactcccg acacggctgt acaaggcctc tgccatcacc ttgcaccgca gcatctatgt
     1621 gctggggggc atggctgtca gctcagggag gagtctggtc agtcacaatg tctacatctt
     1681 ctccctgaaa ctcaatcagt ggaggctggg ggagcccatg ctggtggccc gctactccca
     1741 cagaagcact gcccataaga acttcatctt ctccatcggg gggattggag aagggcagga
     1801 gctcatgggc tccatggaaa ggtatgacag catctgcaat gtctgggaga gtatggccag
     1861 catgcccgtg ggggtgctcc accccgcagt cgctgtgaaa gaccaaagac tctatctctt
     1921 tggaggagag gacatcatgc agaaccctgt gcgccttatc caggtttatc acatttccag
     1981 aaactcgtgg ttcaaaatgg agacaagaat gatcaagaac gtgtgtgccc ctgcagtggt
     2041 gcttggggag cggattgtca ttgtgggagg ttacacaagg aggattcttg cttatgaccc
     2101 tcaatccaac aaatttgtca aatgtgcgga catgaaagac cggaggatgc accatggggc
     2161 cacagtgatg ggaaacaaac tctacgtgac gggcgggcgg cggctgacca cggactgcaa
     2221 cattgaggac tccgcctcct tcgattgcta cgaccccgag acggacacct ggacatccca
     2281 gggacagctg ccgcacaagc tctttgacca tgcctgcctc actctccagt gcataccccg
     2341 cacgtctggc ctcccatacc cctacgacgt gcctgactac gccggcggcg gtggttcagg
     2401 cggtggcggt tccggtggtg ggggatcagt ccaactggtg gagtctggtg gcgctttggt
     2461 gcagccaggt ggctctctgc gtttgtcctg tgccgcttct ggcttcccag tgaaccgcta
     2521 ttccatgcgc tggtatcgcc aggctccagg caaagagcgt gagtgggtag ccggtatgtc
     2581 cagcgcgggt gatcgtagct cctatgaaga ctccgtgaag ggccgtttca ccatcagccg
     2641 tgacgatgcc cgtaacacgg tgtatctgca aatgaacagc ttgaaacctg aagatacggc
     2701 cgtgtattac tgtaatgtga acgtgggctt cgagtattgg ggccaaggca cccaggtcac
     2761 cgtctccagc taagatcctt gacttgcggc cgcaactccc acctgcaaca tgcgtgactg
     2821 actgaggccg cgactctaga gtcgacctgc atctaggcgc cggaattaga tctctcgagg
     2881 ttaacgaatt ctaccgggta ggggaggcgc ttttcccaag gcagtctgga gcatgcgctt
     2941 tagcagcccc gctgggcact tggcgctaca caagtggcct ctggcctcgc acacattcca
     3001 catccaccgg taggcgccaa ccggctccgt tctttggtgg ccccttcgcg ccaccttcta
     3061 ctcctcccct agtcaggaag ttcccccccg ccccgcagct cgcgtcgtgc aggacgtgac
     3121 aaatggaagt agcacgtctc actagtctcg tgcagatgga cagcaccgct gagcaatgga
     3181 agcgggtagg cctttggggc agcggccaat agcagctttg ctccttcgct ttctgggctc
     3241 agaggctggg aaggggtggg tccgggggcg ggctcagggg cgggctcagg ggcggggcgg
     3301 gcgcccgaag gtcctccgga ggcccggcat tctgcacgct tcaaaagcgc acgtctgccg
     3361 cgctgttctc ctcttcctca tctccgggcc tttcgacctg cagcccaagc ttaccatggt
     3421 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3481 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3541 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3601 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     3661 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3721 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     3781 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     3841 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     3901 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     3961 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     4021 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     4081 cgagggccgc cactccaccg gcggcatgga cgagctgtac aaggcaacaa acttctctct
     4141 gctgaaacaa gccggagatg tcgaagagaa tcctggaccg atgaccgagt acaagcccac
     4201 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     4261 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     4321 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     4381 ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     4441 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     4501 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     4561 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     4621 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     4681 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
     4741 accgcgcacc tggtgcatga cccgcaagcc cggtgcctga tcttatcgat aatcaacctc
     4801 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4861 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     4921 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     4981 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5041 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5101 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5161 acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg
     5221 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5281 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     5341 ctcagacgag tcggatctcc ctttgggccg cctccccgca tcgataccgt cgaccaaggc
     5401 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5461 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5521 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     5581 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     5641 cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt
     5701 ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg
     5761 cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc
     5821 ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt
     5881 tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct
     5941 agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt
     6001 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc
     6061 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc
     6121 gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta
     6181 ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag
     6241 tacaatctag ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     6301 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     6361 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     6421 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     6481 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     6541 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     6601 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     6661 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     6721 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     6781 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     6841 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     6901 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     6961 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     7021 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     7081 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     7141 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     7201 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     7261 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     7321 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     7381 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     7441 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     7501 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     7561 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     7621 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     7681 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     7741 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
     7801 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     7861 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     7921 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     7981 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     8041 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     8101 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     8161 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     8221 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     8281 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     8341 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     8401 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     8461 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     8521 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8581 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8641 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8701 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8761 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     8821 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     8881 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     8941 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     9001 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     9061 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     9121 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     9181 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     9241 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     9301 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     9361 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     9421 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     9481 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     9541 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     9601 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     9661 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     9721 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     9781 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     9841 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     9901 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     9961 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
    10021 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
    10081 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
    10141 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
    10201 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
    10261 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
    10321 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
    10381 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
    10441 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
    10501 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    10561 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    10621 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    10681 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
    10741 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctag tggtttcatt
    10801 tactctaaac ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa
    10861 tatgtactac aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc
    10921 cttgatctgt ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca
    10981 gggccagggg tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag
    11041 ccagataagg tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc
    11101 ctgcatggga tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta
    11161 gcatttcatc acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga
    11221 gcttgctaca agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact
    11281 ggggagtggc gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc
    11341 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
    11401 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
    11461 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
    11521 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
    11581 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
    11641 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
    11701 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
    11761 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
    11821 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
    11881 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
    11941 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
    12001 aaaagtaaga ccaccgcaca gcaagcggcc ggtgatcttc agacctggac gatatatatg
    12061 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
    12121 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
    12181 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
    12241 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
    12301 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
    12361 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
    12421 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
    12481 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
    12541 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
    12601 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
    12661 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
    12721 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
    12781 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
    12841 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtcg
//
