LOCUS       V033960                12921 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   V033960
VERSION     V033960
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    25..142
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
                     /label="cPPT/CTS"
     protein_bind    210..228
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    245..263
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    281..299
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    317..335
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    352..370
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     protein_bind    388..406
                     /bound_moiety="tetracycline repressor TetR"
                     /gene="tetO"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /label="tet operator"
     promoter        418..456
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
                     /label="minimal CMV promoter"
     promoter        524..542
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     CDS             614..2377
                     /label="ASB15(NM_080928)"
                     /note="ASB15(NM_080928)"
                     /gene="ASB15"
     CDS             2378..2404
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /transl_table=1
                     /translation="YPYDVPDYA"
                     /label="HA"
     CDS             2450..2791
                     /codon_start=1
                     /product="GFP-binding fragment of a single-chain camelid
                     antibody (Rothbauer et al., 2008)"
                     /transl_table=1
                     /translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
                     WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYCNVNVGFEYW
                     GQGTQVTVSS"
                     /label="GFP nanobody"
     promoter        2917..3416
                     /note="mouse phosphoglycerate kinase 1 promoter"
                     /label="PGK promoter"
     CDS             3437..4144
                     /codon_start=1
                     /note="mammalian codon-optimized"
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /transl_table=1
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
                     /label="mCherry"
     CDS             4145..4201
                     /codon_start=1
                     /note="Eukaryotic ribosomes fail to insert a peptide bond
                     between the Gly and Pro residues, yielding separate
                     polypeptides."
                     /product="2A peptide from porcine teschovirus-1
                     polyprotein"
                     /transl_table=1
                     /translation="ATNFSLLKQAGDVEENPGP"
                     /label="P2A"
     CDS             4202..4801
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /note="confers resistance to puromycin"
                     /product="puromycin N-acetyltransferase"
                     /transl_table=1
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA*"
                     /label="PuroR"
     misc_feature    4812..5400
                     /note="woodchuck hepatitis virus&nbsp;posttranscriptional
                     regulatory element"
                     /label="WPRE"
     LTR             5463..5696
                     /note="self-inactivating&nbsp;3' long terminal repeat (LTR)
                     from HIV-1"
                     /label="3' LTR (ΔU3)"
     promoter        complement(5721..5739)
                     /note="promoter for bacteriophage SP6 RNA polymerase"
                     /label="SP6 promoter"
     promoter        6528..6632
                     /gene="bla"
                     /label="AmpR promoter"
     CDS             6633..7493
                     /codon_start=1
                     /gene="bla"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /product="β-lactamase"
                     /transl_table=1
                     /translation="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEY
                     SPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDR
                     WEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRS
                     ALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGA
                     SLIKHW*"
                     /label="AmpR"
     rep_origin      7664..8252
                     /direction=RIGHT
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
                     /label="ori"
     promoter        8498..8827
                     /note="SV40 enhancer and early promoter"
                     /label="SV40 promoter"
     intron          9961..10026
                     /note="SV40 (simian virus 40) small t antigen intron"
                     /label="small t intron"
     CDS             10156..10176
                     /codon_start=1
                     /locus_tag=""
                     /note=""
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /protein_id=""
                     /transl_table=1
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     polyA_signal    10601..10735
                     /note="SV40 polyadenylation signal"
                     /label="SV40 poly(A) signal"
     LTR             10904..11537
                     /note="3' long terminal repeat (LTR) from HIV-1"
                     /label="3' LTR"
     misc_feature    11584..11709
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
                     /label="HIV-1 Ψ"
     misc_feature    12201..12434
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
                     /label="RRE"
     CDS             12619..12663
                     /codon_start=1
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /product="antigenic peptide corresponding to amino acids
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
                     et al., 2013)"
                     /transl_table=1
                     /translation="KNEQELLELDKWASL"
                     /label="gp41 peptide"
ORIGIN
        1 ccaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
       61 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
      121 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagaga attcacgtgc
      181 tgcaggtccg aggttctaga cgagtttact ccctatcagt gatagagaac gatgtcgagt
      241 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
      301 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
      361 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
      421 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      481 tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg actcactata
      541 gggagaccca agctgtcaga tctcgagctc aagcttcgaa ttctagggcc gcggcagtga
      601 actccaagcc accatggata ctaatgatga ccctgatgaa gaccatctta caagttatga
      661 tattcagcta agtattcaag aatccattga agccagcaag actgcacttt gtcctgaaag
      721 atttgtaccc ctaagtgctc aaaacagaaa acttgtggag gccataaaac aaggtcacat
      781 tcctgagctc caggagtatg taaaatataa atatgcaatg gatgaagctg atgaaaaagg
      841 atggtttcca ttgcatgaag ctgttgttca acccattcaa caaatacttg agattgttct
      901 ggatgcatcc tataagacac tctgggaatt caagacctgt gatggagaaa cacccttgac
      961 tttggcagtc aaagctggtc tggtggaaaa tgtaagaact ttattagaaa agggagtgtg
     1021 gcccaacaca aaaaatgata aaggagagac cccccttctg attgctgtga aaaagggctc
     1081 ctatgacatg gtgtcgactc tgatcaaaca taacactagc ctagaccagc cctgtgtcaa
     1141 gcgatggtca gcaatgcatg aagcagccaa gcaaggccga aaagatatcg tagctctgct
     1201 gctgaaacat ggaggcaatg tccacctgag agatggattt ggagtcacac cactaggcgt
     1261 cgctgccgag tatggtcact gtgacgtgtt agaacatcta atccacaaag gtggtgatgt
     1321 gcttgctttg gcggatgatg gggcgtcggt gctgtttgag gcagcaggag gtggcaatcc
     1381 cgactgcatt tccctcctgc tggaatatgg aggaagcgga aatgtaccta accgagcagg
     1441 acatcttcct atacaccgag ctgcctatga ggggcattat cttgcactga aatatcttat
     1501 cccagtaaca tctaaaaatg caattcggaa aagtgggcta acaccaattc actcagcagc
     1561 agatggacaa aatgcacagt gtctagaact gctcattgaa aatggttttg atgtcaacac
     1621 tctacttgct gaccacattt cccagagcta tgacgatgag aggaagactg cgctgtattt
     1681 tggcgtttct aataatgacg ttcattgcac agaagtcctt ctggctgcag gtgcagaccc
     1741 aaacttagat cccctcaact gtctacttgt tgcagtgagg gccaataatt atgaaattgt
     1801 caggctgctt ctctcccatg gagctaatgt caattgttat tttatgcatg tgaatgacac
     1861 tcgtttcccc agtgtcattc aatatgctct aaacgacgag gtaatgctga ggctattgct
     1921 gaataatggc tatcaagtgg agatgtgctt tgactgcatg catggtgaca tctttggaaa
     1981 ttcatttgtg tggtcagaga tacaggaaga ggtgctgcca ggatggacat cttgtgtaat
     2041 aaaagataac ccgttctgtg agtttattac agttccttgg atgaagcact tggtaggcag
     2101 agttactcgt gtactaatag attacatgga ttatgttcct ctgtgtgcta aactgaagtc
     2161 tgcactagaa gtacagagag aatggccaga aatccgccaa atactagaga atccttgttc
     2221 attgaagcat ttgtgtcggt taaaaattcg aaggcttatg ggtctccaga aactctgcca
     2281 gccagcctca gtggagaagc ttcctctacc accagctatt caaagataca tattatttaa
     2341 agagtatgat ctctatggac aagagctaaa attgacatac ccctacgacg tgcctgacta
     2401 cgccggcggc ggtggttcag gcggtggcgg ttccggtggt gggggatcag tccaactggt
     2461 ggagtctggt ggcgctttgg tgcagccagg tggctctctg cgtttgtcct gtgccgcttc
     2521 tggcttccca gtgaaccgct attccatgcg ctggtatcgc caggctccag gcaaagagcg
     2581 tgagtgggta gccggtatgt ccagcgcggg tgatcgtagc tcctatgaag actccgtgaa
     2641 gggccgtttc accatcagcc gtgacgatgc ccgtaacacg gtgtatctgc aaatgaacag
     2701 cttgaaacct gaagatacgg ccgtgtatta ctgtaatgtg aacgtgggct tcgagtattg
     2761 gggccaaggc acccaggtca ccgtctccag ctaagatcct tgacttgcgg ccgcaactcc
     2821 cacctgcaac atgcgtgact gactgaggcc gcgactctag agtcgacctg catctaggcg
     2881 ccggaattag atctctcgag gttaacgaat tctaccgggt aggggaggcg cttttcccaa
     2941 ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac acaagtggcc
     3001 tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg ttctttggtg
     3061 gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc gccccgcagc
     3121 tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc gtgcagatgg
     3181 acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa tagcagcttt
     3241 gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc gggctcaggg
     3301 gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca ttctgcacgc
     3361 ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc ctttcgacct
     3421 gcagcccaag cttaccatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     3481 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     3541 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     3601 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     3661 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     3721 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     3781 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     3841 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     3901 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     3961 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     4021 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     4081 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     4141 caaggcaaca aacttctctc tgctgaaaca agccggagat gtcgaagaga atcctggacc
     4201 gatgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4261 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4321 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4381 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4441 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4501 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     4561 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     4621 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     4681 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     4741 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     4801 atcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     4861 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     4921 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     4981 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     5041 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     5101 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     5161 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     5221 cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     5281 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     5341 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     5401 atcgataccg tcgaccaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     5461 actggaaggg ctaattcact cccaacgaag acaagatctg ctttttgctt gtactgggtc
     5521 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     5581 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     5641 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat
     5701 ctagaattaa ttccgtgtat tctatagtgt cacctaaatc gtatgtgtat gatacataag
     5761 gttatgtatt aattgtagcc gcgttctaac gacaatatgt acaagcctaa ttgtgtagca
     5821 tctggcttac tgaagcagac cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg
     5881 gttggacgaa ccttctgagt ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa
     5941 ccaatcagca gggtcatcgc tagccagatc ctctacgccg gacgcatcgt ggccggcatc
     6001 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
     6061 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
     6121 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
     6181 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
     6241 cgtcgaatgg tgcactctca gtacaatcta gctctgatgc cgcatagtta agccagcccc
     6301 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     6361 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     6421 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     6481 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     6541 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     6601 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     6661 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     6721 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     6781 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     6841 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     6901 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     6961 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7021 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7081 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7141 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7201 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     7261 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     7321 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     7381 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     7441 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     7501 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     7561 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     7621 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     7681 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     7741 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     7801 caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     7861 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     7921 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     7981 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     8041 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     8101 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     8161 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     8221 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     8281 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     8341 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     8401 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     8461 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     8521 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     8581 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     8641 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     8701 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     8761 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     8821 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     8881 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     8941 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     9001 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     9061 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     9121 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
     9181 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     9241 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     9301 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     9361 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     9421 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     9481 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     9541 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     9601 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     9661 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
     9721 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
     9781 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
     9841 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
     9901 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     9961 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
    10021 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
    10081 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
    10141 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
    10201 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
    10261 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
    10321 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
    10381 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
    10441 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
    10501 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
    10561 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
    10621 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
    10681 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
    10741 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
    10801 caattaccta gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    10861 aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg gctaattcac
    10921 tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
    10981 gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt tggatggtgc
    11041 tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg agagaacacc
    11101 agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtgttagag
    11161 tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca tccggagtac
    11221 ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga ctttccaggg
    11281 aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat ataagcagct
    11341 gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
    11401 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
    11461 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
    11521 gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag
    11581 gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg
    11641 cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc
    11701 gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg
    11761 ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa
    11821 cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga
    11881 cagctacaac catcccttca gacaggatca gaagaactta gatcattata taatacagta
    11941 gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac
    12001 aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc cggtgatctt
    12061 cagacctgga cgatatatat gagggacaat tggagaagtg aattatataa atataaagta
    12121 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    12181 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    12241 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    12301 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    12361 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    12421 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    12481 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    12541 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    12601 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    12661 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    12721 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    12781 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    12841 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    12901 gtgaacggat ctcgacggtc g
//
