LOCUS       .                       9409 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        9..235
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    463..588
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1081..1314
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1499..1543
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1692..1733
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1810..1926
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2131..3312
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
     CDS             3398..4108
                     /label="TagRFP"
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
     misc_feature    4241..4824
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4848..5243
                     /label="BSD"
                     /note="blasticidin S deaminase"
     misc_feature    5261..5849
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5936..6169
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6241..6375
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6402..6537
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6558..6576)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6586..6602)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6744..7199
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7225..7329
                     /label="AmpR promoter"
     CDS             7330..8187
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      8361..8949
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9237..9258
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9273..9303
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9311..9327
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     9335..9351
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9372..9390
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     3301 caggtgtcgt gaaaactacc cctctagagc caccatggag acagatacac tgctgctgtg
     3361 ggtgctgctg ctgtgggtgc ccggcagcac cggggacatg gtgtctaagg gcgaagagct
     3421 gattaaggag aacatgcaca tgaagctgta catggagggc accgtgaaca accaccactt
     3481 caagtgcaca tccgagggcg aaggcaagcc ctacgagggc acccagacca tgagaatcaa
     3541 ggtggtcgag ggcggccctc tccccttcgc cttcgacatc ctggctacca gcttcatgta
     3601 cggcagcaga accttcatca accacaccca gggcatcccc gacttcttta agcagtcctt
     3661 ccctgagggc ttcacatggg agagagtcac cacatacgaa gacgggggcg tgctgaccgc
     3721 tacccaggac accagcctcc aggacggctg cctcatctac aacgtcaaga tcagaggggt
     3781 gaacttccca tccaacggcc ctgtgatgca gaagaaaaca ctcggctggg aggccaacac
     3841 cgagatgctg taccccgctg acggcggcct ggaaggcaga agcgacatgg ccctgaagct
     3901 cgtgggcggg ggccacctga tctgcaactt caagaccaca tacagatcca agaaacccgc
     3961 taagaacctc aagatgcccg gcgtctacta tgtggaccac agactggaaa gaatcaagga
     4021 ggccgacaaa gagacctacg tcgagcagca cgaggtggct gtggccagat actgcgacct
     4081 ccctagcaaa ctggggcaca aacttaatgg gggcggtggg tccgacatct acatctgggc
     4141 ccccctggcc ggcacctgcg gggtgctgct gctgagcctg gtcattacac tgtaaggatc
     4201 cacgcgtctc cggcctaggg ataacagggt aatccgctag cccctctccc tccccccccc
     4261 ctaacgttac tggccgaagc cgcttggaat aaggccggtg tgcgtttgtc tatatgttat
     4321 tttccaccat attgccgtct tttggcaatg tgagggcccg gaaacctggc cctgtcttct
     4381 tgacgagcat tcctaggggt ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg
     4441 tcgtgaagga agcagttcct ctggaagctt cttgaagaca aacaacgtct gtagcgaccc
     4501 tttgcaggca gcggaacccc ccacctggcg acaggtgcct ctgcggccaa aagccacgtg
     4561 tataagatac acctgcaaag gcggcacaac cccagtgcca cgttgtgagt tggatagttg
     4621 tggaaagagt caaatggctc tcctcaagcg tattcaacaa ggggctgaag gatgcccaga
     4681 aggtacccca ttgtatggga tctgatctgg ggcctcggta cacatgcttt acatgtgttt
     4741 agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa
     4801 aaacacgatg ataatatggc cacactagag atccaccggt cgccaccatg gccaagcctt
     4861 tgtctcaaga agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca
     4921 tctctgaaga ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg
     4981 gtgtcaatgt atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg
     5041 ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg
     5101 gcatcttgag cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca
     5161 aagccatagt gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc
     5221 cctctggtta tgtgtgggag ggctaagcgg ccgcgtcgac aatcaacctc tggattacaa
     5281 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     5341 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     5401 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     5461 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     5521 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     5581 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     5641 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     5701 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     5761 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     5821 tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca
     5881 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
     5941 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
     6001 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
     6061 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
     6121 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
     6181 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
     6241 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6301 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6361 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
     6421 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     6481 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     6541 ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt
     6601 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     6661 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     6721 gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
     6781 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
     6841 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
     6901 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
     6961 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     7021 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     7081 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     7141 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta
     7201 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     7261 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     7321 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     7381 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     7441 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     7501 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     7561 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     7621 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     7681 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     7741 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     7801 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     7861 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     7921 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     7981 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     8041 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     8101 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     8161 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     8221 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     8281 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     8341 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     8401 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     8461 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     8521 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     8581 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     8641 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     8701 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     8761 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     8821 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     8881 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     8941 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     9001 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     9061 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     9121 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     9181 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     9241 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     9301 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     9361 gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgc
//
