LOCUS       .                       9373 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        9..235
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     misc_feature    463..588
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1081..1314
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1499..1543
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1692..1733
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1810..1926
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2131..3312
                     /label="EF-1α promoter"
                     /note="strong constitutive promoter for human elongation
                     factor EF-1α"
     CDS             3398..4072
                     /label="DsRed1"
                     /note="wild-type DsRed"
     misc_feature    4205..4788
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4812..5207
                     /label="BSD"
                     /note="blasticidin S deaminase"
     misc_feature    5225..5813
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5900..6133
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6205..6339
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6366..6501
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6522..6540)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6550..6566)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6708..7163
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7189..7293
                     /label="AmpR promoter"
     CDS             7294..8151
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      8325..8913
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9201..9222
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9237..9267
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9275..9291
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     9299..9315
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9336..9354
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     3121 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     3181 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     3241 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     3301 caggtgtcgt gaaaactacc cctctagagc caccatggag acagatacac tgctgctgtg
     3361 ggtgctgctg ctgtgggtgc ccggcagcac cggggacgtg aggagcagca agaacgtgat
     3421 caaggagttc atgcggttca aggtgaggat ggagggcacc gtgaacgggc acgagttcga
     3481 gatcgagggg gagggggagg ggaggcccta cgaggggcac aacaccgtga agctgaaggt
     3541 gaccaagggc ggccccctgc ccttcgcctg ggacatcctg agcccccagt tccagtacgg
     3601 gagcaaggtg tacgtgaagc accccgccga tatccccgat tacaagaagc tgagcttccc
     3661 cgagggcttc aagtgggaga gagtgatgaa cttcgaggat gggggcgtgg tgaccgtgac
     3721 ccaggactcc agcctgcagg atgggtgctt catctacaag gtgaagttca tcggcgtgaa
     3781 cttccccagc gatggccccg tgatgcagaa aaagaccatg gggtgggagg ccagcaccga
     3841 gcggctgtac ccacgggacg gggtgctgaa gggggagatc cacaaggccc tgaagctgaa
     3901 ggacgggggg cactacctgg tggagttcaa gagcatctac atggccaaga agcccgtgca
     3961 gctgcccggc tactactacg tggatagcaa gctggatatc acaagccaca acgaggacta
     4021 cacaatcgtg gagcagtacg agaggacaga ggggaggcac cacctgttcc tggggggcgg
     4081 tgggtccgac atctacatct gggcccccct ggccggcacc tgcggggtgc tgctgctgag
     4141 cctggtcatt acactgtaag gatccacgcg tctccggcct agggataaca gggtaatccg
     4201 ctagcccctc tccctccccc ccccctaacg ttactggccg aagccgcttg gaataaggcc
     4261 ggtgtgcgtt tgtctatatg ttattttcca ccatattgcc gtcttttggc aatgtgaggg
     4321 cccggaaacc tggccctgtc ttcttgacga gcattcctag gggtctttcc cctctcgcca
     4381 aaggaatgca aggtctgttg aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa
     4441 gacaaacaac gtctgtagcg accctttgca ggcagcggaa ccccccacct ggcgacaggt
     4501 gcctctgcgg ccaaaagcca cgtgtataag atacacctgc aaaggcggca caaccccagt
     4561 gccacgttgt gagttggata gttgtggaaa gagtcaaatg gctctcctca agcgtattca
     4621 acaaggggct gaaggatgcc cagaaggtac cccattgtat gggatctgat ctggggcctc
     4681 ggtacacatg ctttacatgt gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc
     4741 acggggacgt ggttttcctt tgaaaaacac gatgataata tggccacact agagatccac
     4801 cggtcgccac catggccaag cctttgtctc aagaagaatc caccctcatt gaaagagcaa
     4861 cggctacaat caacagcatc cccatctctg aagactacag cgtcgccagc gcagctctct
     4921 ctagcgacgg ccgcatcttc actggtgtca atgtatatca ttttactggg ggaccttgtg
     4981 cagaactcgt ggtgctgggc actgctgctg ctgcggcagc tggcaacctg acttgtatcg
     5041 tcgcgatcgg aaatgagaac aggggcatct tgagcccctg cggacggtgc cgacaggtgc
     5101 ttctcgatct gcatcctggg atcaaagcca tagtgaagga cagtgatgga cagccgacgg
     5161 cagttgggat tcgtgaattg ctgccctctg gttatgtgtg ggagggctaa gcggccgcgt
     5221 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     5281 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     5341 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     5401 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     5461 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     5521 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     5581 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
     5641 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     5701 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     5761 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
     5821 tcgagctcgg tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     5881 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     5941 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     6001 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     6061 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     6121 aaaatctcta gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag
     6181 aaatgaatat cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa
     6241 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6301 ttgtccaaac tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc
     6361 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     6421 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     6481 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
     6541 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
     6601 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
     6661 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
     6721 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
     6781 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
     6841 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
     6901 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
     6961 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
     7021 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
     7081 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
     7141 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
     7201 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     7261 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     7321 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     7381 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     7441 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     7501 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     7561 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     7621 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     7681 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     7741 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     7801 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     7861 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     7921 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     7981 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     8041 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     8101 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     8161 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     8221 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     8281 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     8341 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8401 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8461 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8521 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8581 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8641 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8701 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8761 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8821 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8881 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8941 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     9001 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     9061 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     9121 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     9181 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     9241 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     9301 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     9361 aagctggagc tgc
//
