LOCUS       .                       9540 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        9..235
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             236..416
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    463..588
                     /label="HIV-1 Ψ"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1081..1314
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1499..1543
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1692..1733
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    1810..1926
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2131..2510
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2511..2714
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2800..3510
                     /label="TagRFP"
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
     CDS             3607..4323
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     misc_feature    4372..4955
                     /label="IRES2"
                     /note="internal ribosome entry site (IRES) of the
                     encephalomyocarditis virus (EMCV)"
     CDS             4979..5374
                     /label="BSD"
                     /note="blasticidin S deaminase"
     misc_feature    5392..5980
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6067..6300
                     /label="3' LTR (ΔU3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6372..6506
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      6533..6668
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(6689..6707)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6717..6733)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      6875..7330
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7356..7460
                     /label="AmpR promoter"
     CDS             7461..8318
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      8492..9080
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    9368..9389
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9404..9434
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    9442..9458
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     9466..9482
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        9503..9521
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc
     2101 atcactgagg tggagaagag catgcgtgag gacattgatt attgactagt tattaatagt
     2161 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     2221 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     2281 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     2341 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     2401 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     2461 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     2521 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     2581 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     2641 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     2701 atataagcag agctaaacta cccctctaga gccaccatgg agacagatac actgctgctg
     2761 tgggtgctgc tgctgtgggt gcccggcagc accggggaca tggtgtctaa gggcgaagag
     2821 ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgaa caaccaccac
     2881 ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac catgagaatc
     2941 aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac cagcttcatg
     3001 tacggcagca gaaccttcat caaccacacc cagggcatcc ccgacttctt taagcagtcc
     3061 ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg cgtgctgacc
     3121 gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa gatcagaggg
     3181 gtgaacttcc catccaacgg ccctgtgatg cagaagaaaa cactcggctg ggaggccaac
     3241 accgagatgc tgtaccccgc tgacggcggc ctggaaggca gaagcgacat ggccctgaag
     3301 ctcgtgggcg ggggccacct gatctgcaac ttcaagacca catacagatc caagaaaccc
     3361 gctaagaacc tcaagatgcc cggcgtctac tatgtggacc acagactgga aagaatcaag
     3421 gaggccgaca aagagaccta cgtcgagcag cacgaggtgg ctgtggccag atactgcgac
     3481 ctccctagca aactggggca caaacttaat gggggcggtg ggtccgacat ctacatctgg
     3541 gcccccctgg ccggcacctg cggggtgctg ctgctgagcc tggtcattac actgggaggt
     3601 ggatctatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3661 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     3721 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3781 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3841 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3901 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3961 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     4021 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     4081 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     4141 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     4201 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     4261 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     4321 aagtaaggat ccacgcgtct ccggcctagg gataacaggg taatccgcta gcccctctcc
     4381 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
     4441 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
     4501 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
     4561 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
     4621 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
     4681 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
     4741 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
     4801 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt acacatgctt
     4861 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
     4921 tttcctttga aaaacacgat gataatatgg ccacactaga gatccaccgg tcgccaccat
     4981 ggccaagcct ttgtctcaag aagaatccac cctcattgaa agagcaacgg ctacaatcaa
     5041 cagcatcccc atctctgaag actacagcgt cgccagcgca gctctctcta gcgacggccg
     5101 catcttcact ggtgtcaatg tatatcattt tactggggga ccttgtgcag aactcgtggt
     5161 gctgggcact gctgctgctg cggcagctgg caacctgact tgtatcgtcg cgatcggaaa
     5221 tgagaacagg ggcatcttga gcccctgcgg acggtgccga caggtgcttc tcgatctgca
     5281 tcctgggatc aaagccatag tgaaggacag tgatggacag ccgacggcag ttgggattcg
     5341 tgaattgctg ccctctggtt atgtgtggga gggctaagcg gccgcgtcga caatcaacct
     5401 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5461 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5521 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5581 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5641 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5701 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5761 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     5821 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5881 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5941 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggaattcg agctcggtac
     6001 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     6061 ggggactgga agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg
     6121 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     6181 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6241 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6301 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     6361 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6421 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6481 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     6541 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6601 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6661 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     6721 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6781 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6841 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     6901 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6961 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     7021 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     7081 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     7141 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     7201 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7261 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7321 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7381 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7441 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7501 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7561 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7621 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7681 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7741 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7801 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7861 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7921 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7981 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     8041 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     8101 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     8161 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     8221 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8281 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8341 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8401 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8461 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8521 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8581 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8641 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8701 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8761 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8821 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8881 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8941 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     9001 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     9061 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     9121 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     9181 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     9241 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     9301 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     9361 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     9421 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     9481 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
//
