LOCUS       40924_28741        9414 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Yeast truncation assay backbone vector pLSK870, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9414)
  AUTHORS   Zan Y, Haag JD, Chen KS, Shepel LA, Wigington D, Wang YR, Hu R, 
            Lopez-Guajardo CC, Brose HL, Porter KI, Leonard RA, Hitt AA, 
            Schommer SL, Elegbede AF, Gould MN.
  TITLE     Production of knockout rats using ENU mutagenesis and a yeast-based 
            screening assay
  JOURNAL   Nat. Biotechnol. 21 (6), 645-651 (2003)
  PUBMED    12754522
REFERENCE   2  (bases 1 to 9414)
  AUTHORS   Chen K-S., Gould MN.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-MAY-2003) Oncology, University of Wisconsin, 1400 
            University Avenue, Madison, WI 53706, USA
REFERENCE   3  (bases 1 to 9414)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9414)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol."; date: "2003"; volume: "21"; issue: "6"; pages: 
            "645-651"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-MAY-2003) Oncology, University of Wisconsin, 1400 University 
            Avenue, Madison, WI 53706, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(70..573)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        610..714
                     /label=AmpR promoter
     CDS             715..1572
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1746..2334
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2622..2643
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2658..2688
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2696..2712
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2720..2736
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2757..2775
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3559..4263
                     /label=ADH1 promoter
                     /note="promoter for alcohol dehydrogenase 1"
     misc_feature    4266..4271
                     /label=HindIII site
                     /note="HindIII site"
     misc_feature    4273..4305
                     /label=encodes rat p53 leader sequence
                     /note="encodes rat p53 leader sequence"
     misc_feature    4305..4312
                     /label=NotI cloning site
                     /note="NotI cloning site"
     CDS             4312..6024
                     /codon_start=1
                     /label=ADE2
                     /note="phosphoribosylaminoimidazole carboxylase, required
                     for adenine biosynthesis"
                     /translation="LDSRTVGILGGGQLGRMIVEAANRLNIKTVILDAENSPAKQISNS
                     NDHVNGSFSNPLDIEKLAEKCDVLTIEIEHVDVPTLKNLQVKHPKLKIYPSPETIRLIQ
                     DKYIQKEHLIKNGIAVTQSVPVEQASETSLLNVGRDLGFPFVLKSRTLAYDGRGNFVVK
                     NKEMIPEALEVLKDRPLYAEKWAPFTKELAVMIVRSVNGLVFSYPIVETIHKDNICDLC
                     YAPARVPDSVQLKAKLLAENAIKSFPGCGIFGVEMFYLETGELLINEIAPRPHNSGHYT
                     IDACVTSQFEAHLRSILDLPMPKNFTSFSTITTNAIMLNVLGDKHTKDKELETCERALA
                     TPGSSVYLYGKESRPNRKVGHINIIASSMAECEQRLNYITGRTDIPIKISVAQKLDLEA
                     MVKPLVGIIMGSDSDLPVMSAACAVLKDFGVPFEVTIVSAHRTPHRMSAYAISASKRGI
                     KTIIAGAGGAAHLPGMVAAMTPLPVIGVPVKGSCLDGVDSLHSIVQMPRGVPVATVAIN
                     NSTNAALLAVRLLGAYDSSYTTKMEQFLLKQEEEVLVKAQKLETVGYEAYLENK"
     terminator      6040..6287
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     promoter        complement(6311..6329)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6336..6352)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      6493..6948
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        7249..7656
                     /label=LEU2 promoter
     CDS             7657..8748
                     /codon_start=1
                     /label=LEU2
                     /note="3-isopropylmalate dehydrogenase, required for
                     leucine biosynthesis"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa tgatggaata
      121 atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat tttagaaagt
      181 aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa ggtttaaaaa
      241 atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc agattaaata
      301 gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg tgtgtggtct
      361 tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa gagcaagata
      421 aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa aacaaaaact
      481 attttttctt taatttcttt ttttactttc tatttttaat ttatatattt atattaaaaa
      541 atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg cacttttcgg
      601 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
      661 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
      721 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
      781 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
      841 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
      901 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
      961 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     1021 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     1081 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     1141 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     1201 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     1261 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     1321 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     1381 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     1441 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     1501 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     1561 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     1621 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     1681 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     1741 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     1801 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     1861 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     1921 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     1981 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2041 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2101 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     2161 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     2221 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     2281 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     2341 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     2401 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     2461 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     2521 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     2581 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     2641 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     2701 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcgaaatt
     2761 aaccctcact aaagggaaca aaagctggta ccgggccccc cctcgagatc cgggatcgaa
     2821 gaaatgatgg taaatgaaat aggaaatcaa ggagcatgaa ggcaaaagac aaatataagg
     2881 gtcgaacgaa aaataaagtg aaaagtgttg atatgatgta tttggctttg cggcgccgaa
     2941 aaaacgagtt tacgcaattg cacaatcatg ctgactctgt ggcggacccg cgctcttgcc
     3001 ggcccggcga taacgctggg cgtgaggctg tgcccggcgg agttttttgc gcctgcattt
     3061 tccaaggttt accctgcgct aaggggcgag attggagaag caataagaat gccggttggg
     3121 gttgcgatga tgacgaccac gacaactggt gtcattattt aagttgccga aagaacctga
     3181 gtgcatttgc aacatgagta tactagaaga atgagccaag acttgcgaga cgcgagtttg
     3241 ccggtggtgc gaacaataga gcgaccatga ccttgaaggt gagacgcgca taaccgctag
     3301 agtactttga agaggaaaca gcaatagggt tgctaccagt ataaatagac aggtacatac
     3361 aacactggaa atggttgtct gtttgagtac gctttcaatt catttgggtg tgcactttat
     3421 tatgttacaa tatggaaggg aactttacac ttctcctatg cacatatatt aattaaagtc
     3481 caatgctagt agagaagggg ggtaacaccc ctccgcgctc ttttccgatt tttttctaaa
     3541 ccgtggaata tttcggatat ccttttgttg tttccgggtg tacaatatgg acttcctctt
     3601 ttctggcaac caaacccata catcgggatt cctataatac cttcgttggt ctccctaaca
     3661 tgtaggtggc ggaggggaga tatacaatag aacagatacc agacaagaca taatgggcta
     3721 aacaagacta caccaattac actgcctcat tgatggtggt acataacgaa ctaatactgt
     3781 agccctagac ttgatagcca tcatcatatc gaagtttcac tacccttttt ccatttgcca
     3841 tctattgaag taataatagg cgcatgcaac ttcttttctt tttttttctt ttctctctcc
     3901 cccgttgttg tctcaccata tccgcaatga caaaaaaatg atggaagaca ctaaaggaaa
     3961 aaattaacga caaagacagc accaacagat gtcgttgttc cagagctgat gaggggtatc
     4021 tcgaagcaca cgaaactttt tccttccttc attcacgcac actactctct aatgagcaac
     4081 ggtatacggc cttccttcca gttacttgaa tttgaaataa aaaaaagttt gctgtcttgc
     4141 tatcaagtat aaatagacct gcaattatta atcttttgtt tcctcgtcat tgttctcgtt
     4201 ccctttcttc cttgtttctt tttctgcaca atatttcaag ctataccaag catacaatca
     4261 actgcaagct tcatggagga ttcacagtcg gatatgagca tcgagcggcc gctggattct
     4321 agaacagttg gtatattagg agggggacaa ttgggacgta tgattgttga ggcagcaaac
     4381 aggctcaaca ttaagacggt aatactagat gctgaaaatt ctcctgccaa acaaataagc
     4441 aactccaatg accacgttaa tggctccttt tccaatcctc ttgatatcga aaaactagct
     4501 gaaaaatgtg atgtgctaac gattgagatt gagcatgttg atgttcctac actaaagaat
     4561 cttcaagtaa aacatcccaa attaaaaatt tacccttctc cagaaacaat cagattgata
     4621 caagacaaat atattcaaaa agagcattta atcaaaaatg gtatagcagt tacccaaagt
     4681 gttcctgtgg aacaagccag tgagacgtcc ctattgaatg ttggaagaga tttgggtttt
     4741 ccattcgtct tgaagtcgag gactttggca tacgatggaa gaggtaactt cgttgtaaag
     4801 aataaggaaa tgattccgga agctttggaa gtactgaagg atcgtccttt gtacgccgaa
     4861 aaatgggcac catttactaa agaattagca gtcatgattg tgaggtctgt taacggttta
     4921 gtgttttctt acccaattgt agagactatc cacaaggaca atatttgtga cttatgttat
     4981 gcgcctgcta gagttccgga ctccgttcaa cttaaggcga agttgttggc agaaaatgca
     5041 atcaaatctt ttcccggttg tggtatattt ggtgtggaaa tgttctattt agaaacaggg
     5101 gaattgctta ttaacgaaat tgccccaagg cctcacaact ctggacatta taccattgat
     5161 gcttgcgtca cttctcaatt tgaagctcat ttgagatcaa tattggattt gccaatgcca
     5221 aagaatttca catctttctc caccattaca acgaacgcca ttatgctaaa tgttcttgga
     5281 gacaaacata caaaagataa agagctagaa acttgcgaaa gagcattggc gactccaggt
     5341 tcctcagtgt acttatatgg aaaagagtct agacctaaca gaaaagtagg tcacataaat
     5401 attattgcct ccagtatggc ggaatgtgaa caaaggctga actacattac aggtagaact
     5461 gatattccaa tcaaaatctc tgtcgctcaa aagttggact tggaagcaat ggtcaaacca
     5521 ttggttggaa tcatcatggg atcagactct gacttgccgg taatgtctgc cgcatgtgcg
     5581 gttttaaaag attttggcgt tccatttgaa gtgacaatag tctctgctca tagaactcca
     5641 cataggatgt cagcatatgc tatttccgca agcaagcgtg gaattaaaac aattatcgct
     5701 ggagctggtg gggctgctca cttgccaggt atggtggctg caatgacacc acttcctgtc
     5761 atcggtgtgc ccgtaaaagg ttcttgtcta gatggagtag attctttaca ttcaattgtg
     5821 caaatgccta gaggtgttcc agtagctacc gtcgctatta ataatagtac gaacgctgcg
     5881 ctgttggctg tcagactgct tggcgcttat gattcaagtt atacaacgaa aatggaacag
     5941 tttttattaa agcaagaaga agaagttctt gtcaaagcac aaaagttaga aactgtcggt
     6001 tacgaagctt atctagaaaa caagtaacgg ccgccaccat catgtaatta gttatgtcac
     6061 gcttacattc acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac
     6121 ctgaagtcta ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta
     6181 tatttcaaat ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga
     6241 aaaccttgct tgagaaggtt ttgggacgct cgaaggcttt aatttgcaag ctggtggagc
     6301 tccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt
     6361 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     6421 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     6481 aatggcgaat ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     6541 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     6601 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     6661 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     6721 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     6781 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     6841 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     6901 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttcc tgatgcggta
     6961 ttttctcctt acgcatctgt gcggtatttc acaccgcata tcgaccggtc gaggagaact
     7021 tctagtatat ctacatacct aatattattg ccttattaaa aatggaatcc caacaattac
     7081 atcaaaatcc acattctctt caaaatcaat tgtcctgtac ttccttgttc atgtgtgttc
     7141 aaaaacgtta tatttatagg ataattatac tctatttctc aacaagtaat tggttgtttg
     7201 gccgagcggt ctaaggcgcc tgattcaaga aatatcttga ccgcagttaa ctgtgggaat
     7261 actcaggtat cgtaagatgc aagagttcga atctcttagc aaccattatt tttttcctca
     7321 acataacgag aacacacagg ggcgctatcg cacagaatca aattcgatga ctggaaattt
     7381 tttgttaatt tcagaggtcg cctgacgcat ataccttttt caactgaaaa attgggagaa
     7441 aaaggaaagg tgagagcgcc ggaaccggct tttcatatag aatagagaag cgttcatgac
     7501 taaatgcttg catcacaata cttgaagttg acaatattat ttaaggacct attgtttttt
     7561 ccaataggtg gttagcaatc gtcttacttt ctaacttttc ttacctttta catttcagca
     7621 atatatatat atatatttca aggatatacc attctaatgt ctgcccctaa gaagatcgtc
     7681 gttttgccag gtgaccacgt tggtcaagaa atcacagccg aagccattaa ggttcttaaa
     7741 gctatttctg atgttcgttc caatgtcaag ttcgatttcg aaaatcattt aattggtggt
     7801 gctgctatcg atgctacagg tgttccactt ccagatgagg cgctggaagc ctccaagaag
     7861 gctgatgccg ttttgttagg tgctgtgggt ggtcctaaat ggggtaccgg tagtgttaga
     7921 cctgaacaag gtttactaaa aatccgtaaa gaacttcaat tgtacgccaa cttaagacca
     7981 tgtaactttg catccgactc tcttttagac ttatctccaa tcaagccaca atttgctaaa
     8041 ggtactgact tcgttgttgt cagagaatta gtgggaggta tttactttgg taagagaaag
     8101 gaagacgatg gtgatggtgt cgcttgggat agtgaacaat acaccgttcc agaagtgcaa
     8161 agaatcacaa gaatggccgc tttcatggcc ctacaacatg agccaccatt gcctatttgg
     8221 tccttggata aagctaatgt tttggcctct tcaagattat ggagaaaaac tgtggaggaa
     8281 accatcaaga acgaattccc tacattgaag gttcaacatc aattgattga ttctgccgcc
     8341 atgatcctag ttaagaaccc aacccaccta aatggtatta taatcaccag caacatgttt
     8401 ggtgatatca tctccgatga agcctccgtt atcccaggtt ccttgggttt gttgccatct
     8461 gcgtccttgg cctctttgcc agacaagaac accgcatttg gtttgtacga accatgccac
     8521 ggttctgctc cagatttgcc aaagaataag gtcaacccta tcgccactat cttgtctgct
     8581 gcaatgatgt tgaaattgtc attgaacttg cctgaagaag gtaaggccat tgaagatgca
     8641 gttaaaaagg ttttggatgc aggtatcaga actggtgatt taggtggttc caacagtacc
     8701 accgaagtcg gtgatgctgt cgccgaagaa gttaagaaaa tccttgctta aaaagattct
     8761 ctttttttat gatatttgta cataaacttt ataaatgaaa ttcataatag aaacgacacg
     8821 aaattacaaa atggaatatg ttcatagggt agacgaaact atatacgcaa tctacataca
     8881 tttatcaaga aggagaaaaa ggaggatgta aaggaataca ggtaagcaaa ttgatactaa
     8941 tggctcaacg tgataaggaa aaagaattgc actttaacat taatattgac aaggaggagg
     9001 gcaccacaca aaaagttagg tgtaacagaa aatcatgaaa ctatgattcc taatttatat
     9061 attggaggat tttctctaaa aaaaaaaaaa tacaacaaat aaaaaacact caatgacctg
     9121 accatttgat ggagtttaag tcaatacctt cttgaaccat ttcccataat ggtgaaagtt
     9181 ccctcaagaa ttttactctg tcagaaacgg ccttaacgac gtagtcgata tggtgcactc
     9241 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     9301 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     9361 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcga
//
