LOCUS       .                       3891 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        27..311
                     /label="araBAD promoter"
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite
                     direction (Guzman et al., 1995)"
     promoter        complement(1037..1055)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     rep_origin      complement(1070..1458)
                     /label="R6K γ ori"
                     /note="γ replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(1618..2148)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(2337..2365)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     promoter        2492..2510
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     oriT            2664..2773
                     /label="oriT"
                     /note="incP origin of transfer"
     CDS             complement(3016..3891)
                     /label="araC"
                     /note="L-arabinose regulatory protein"
ORIGIN
        1 acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg
       61 ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa
      121 aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta
      181 taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat
      241 agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac
      301 tgtttctcca tacccgtttt tttggaagaa ggagatatac atatgatgac caaaaaacct
      361 gaattttatg ctccagacct aacggaagaa cgcttgcaca tattgagtga aaacctactc
      421 gatgtgctcg atgaggccca tcattactca gaaagtccca atgcgacggc ctggtttaaa
      481 ggcactgcaa attatggttt accacaggga atgctaatcc gtatgcactc agacagtgct
      541 tatccatggc tcacacttgc caaccaaaca atggattaca ctgctagagt tggaaacact
      601 cttgttcaat ttgttgtgga tgaccctcac tcacctcgca agcaacatcg ccttaaacgc
      661 aatgctgttg aaaagcatca aatatctctt gaattagagg aagactatgt tgatacccca
      721 ttgatttggc gattttactt aaatcctata tctaatggtg ttgattattc cccatctatt
      781 tctttgttgg gtttcaacgg caatggcaac gttatatgca gttgggaata cgacacagtt
      841 gtaactggtc cagttgttac agacaaacca gaatcggttg aaattgatga gccattattg
      901 gttcgtaaaa agaaagtaca aaaaaaggtc tctgatgagt aaaagctcga gggaatataa
      961 gtcgacggat cccccgggct gcaggaatct agaccttgag tcgaattcga gctcgacata
     1021 tcgtcgctag ctgttccctt tagtgagggt taattgcgcg caattcccat gtcagccgtt
     1081 aagtgttcct gtgtcactca aaattgcttt gagaggctct aagggcttct cagtgcgtta
     1141 catccctggc ttgttgtcca caaccgttaa accttaaaag ctttaaaagc cttatatatt
     1201 cttttttttc ttataaaact taaaacctta gaggctattt aagttgctga tttatattaa
     1261 ttttattgtt caaacatgag agcttagtac gtgaaacatg agagcttagt acgttagcca
     1321 tgagagctta gtacgttagc catgagggtt tagttcgtta aacatgagag cttagtacgt
     1381 taaacatgag agcttagtac gtgaaacatg agagcttagt acgtactatc aacaggttga
     1441 actgctgatc ttcagatcct ctacgccgga cgcatcgtgg ccggatcaga tctgatatcg
     1501 tcgcagacca aaacgatctc aagaagatca tcttattaat cagataaaat atttctaggc
     1561 accaataact gccttaaaaa aattacgccc cgccctgcca ctcatcgcag tactttaggt
     1621 ggcggtactt gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata
     1681 gagagccact gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag
     1741 cgcgttggcc tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg
     1801 ctcgccggag actgcgagat catagatata gatctcacta cgcggctgct caaacctggg
     1861 cagaacgtaa gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat
     1921 gaatgtctta ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta
     1981 ggtggctacg tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac
     2041 ttggtcaggg ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt
     2101 tttagggcga ctgccctgct gcgtaacatc gttgctgctg cgtaacatcg ttgctgctcc
     2161 ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag
     2221 actgtacaaa aaaacagtca taacaagcca tgaaaaccgc cactgcgccg ttaccaccgc
     2281 tgcgttcggt caaggttctg gaccagttgc gtgagcgcat acgctacttg cattacagtt
     2341 tacgaaccga acaggcttat gtcaaacgtc tcattttcgc caaaagttgg cccagggctt
     2401 cccggtatca acagggacac caggatttat ttattctgcg aagtgatctt ccgtcacagg
     2461 tatttattcg gcgcaattga tatcgcgcgc gtaatacgac tcactatagg gcgaattggg
     2521 taccagcgct tttccgctgc ataaccctgc ttcggggtca ttatagcgat tttttcggta
     2581 tatccatcct ttttcgcacg atatacagga ttttgccaaa gggttcgtgt agactttcct
     2641 tggtgtatcc aacggcgtca gccgggcagg ataggtgaag taggcccacc cgcgagcggg
     2701 tgttccttct tcactgtccc ttattcgcac ctggcggtgc tcaacgggaa tcctgctctg
     2761 cgaggctggc cggcgtcgac ggtatcgata agcttggcat gcctgcagca tacactccgc
     2821 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     2881 cctgacgggc ttgtctgctc ccggcatccg cttacagatc gatgcataat gtgcctgtca
     2941 aatggacgaa gcagggattc tgcaaaccct atgctactcc gtcaagccgt caattgtctg
     3001 attcgttacc aattatgaca acttgacggc tacatcattc actttttctt cacaaccggc
     3061 acggaactcg ctcgggctgg ccccggtgca ttttttaaat acccgcgaga aatagagttg
     3121 atcgtcaaaa ccaacattgc gaccgacggt ggcgataggc atccgggtgg tgctcaaaag
     3181 cagcttcgcc tggctgatac gttggtcctc gcgccagctt aagacgctaa tccctaactg
     3241 ctggcggaaa agatgtgaca gacgcgacgg cgacaagcaa acatgctgtg cgacgctggc
     3301 gatatcaaaa ttgctgtctg ccaggtgatc gctgatgtac tgacaagcct cgcgtacccg
     3361 attatccatc ggtggatgga gcgactcgtt aatcgcttcc atgcgccgca gtaacaattg
     3421 ctcaagcaga tttatcgcca gcagctccga atagcgccct tccccttgcc cggcgttaat
     3481 gatttgccca aacaggtcgc tgaaatgcgg ctggtgcgct tcatccgggc gaaagaaccc
     3541 cgtattggca aatattgacg gccagttaag ccattcatgc cagtaggcgc gcggacgaaa
     3601 gtaaacccac tggtgatacc attcgcgagc ctccggatga cgaccgtagt gatgaatctc
     3661 tcctggcggg aacagcaaaa tatcacccgg tcggcaaaca aattctcgtc cctgattttt
     3721 caccaccccc tgaccgcgaa tggtgagatt gagaatataa cctttcattc ccagcggtcg
     3781 gtcgataaaa aaatcgagat aaccgttggc ctcaatcggc gttaaacccg ccaccagatg
     3841 ggcattaaac gagtatcccg gcagcagggg atcattttgc gcttcagcca t
//
