LOCUS       pLP1.        8889 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Lentiviral packaging plasmid containing the HIV-1 gag and pol genes.
ACCESSION   .
VERSION     .
KEYWORDS    pLP1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        79..458
                     /note="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        459..662
                     /note="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          796..1271
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     CDS             1355..2854
                     /label=HIV-1 gag
                     /note="gag protein from human immunodeficiency virus 1"
     CDS             2650..5658
                     /label=HIV-1 pol
                     /note="pol protein from human immunodeficiency virus 1"
     misc_feature    5686..5919
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     polyA_signal    6150..6544
                     /label=beta-globin poly(A)
                     /note="human beta-globin polyadenylation signal"
     rep_origin      complement(7054..7642)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7816..8673)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8674..8778)
                     /label=AmpR promoter
ORIGIN
        1 ttggcccatt gcatacgttg tatccatatc ataatatgta catttatatt ggctcatgtc
       61 caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
      121 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
      181 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
      241 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
      301 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
      361 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
      421 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
      481 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
      541 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
      601 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag
      661 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      721 gaagacaccg ggaccgatcc agcctcccct cgaagcttac atgtggtacc gagctcggat
      781 cctgagaact tcagggtgag tctatgggac ccttgatgtt ttctttcccc ttcttttcta
      841 tggttaagtt catgtcatag gaaggggaga agtaacaggg tacacatatt gaccaaatca
      901 gggtaatttt gcatttgtaa ttttaaaaaa tgctttcttc ttttaatata cttttttgtt
      961 tatcttattt ctaatacttt ccctaatctc tttctttcag ggcaataatg atacaatgta
     1021 tcatgcctct ttgcaccatt ctaaagaata acagtgataa tttctgggtt aaggcaatag
     1081 caatatttct gcatataaat atttctgcat ataaattgta actgatgtaa gaggtttcat
     1141 attgctaata gcagctacaa tccagctacc attctgcttt tattttatgg ttgggataag
     1201 gctggattat tctgagtcca agctaggccc ttttgctaat catgttcata cctcttatct
     1261 tcctcccaca gctcctgggc aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag
     1321 cacgtgagat ctgaattcga gatctgccgc cgccatgggt gcgagagcgt cagtattaag
     1381 cgggggagaa ttagatcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     1441 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     1501 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     1561 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     1621 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     1681 caaaagtaag aaaaaagcac agcaagcagc agctgacaca ggacacagca atcaggtcag
     1741 ccaaaattac cctatagtgc agaacatcca ggggcaaatg gtacatcagg ccatatcacc
     1801 tagaacttta aatgcatggg taaaagtagt agaagagaag gctttcagcc cagaagtgat
     1861 acccatgttt tcagcattat cagaaggagc caccccacaa gatttaaaca ccatgctaaa
     1921 cacagtgggg ggacatcaag cagccatgca aatgttaaaa gagaccatca atgaggaagc
     1981 tgcagaatgg gatagagtgc atccagtgca tgcagggcct attgcaccag gccagatgag
     2041 agaaccaagg ggaagtgaca tagcaggaac tactagtacc cttcaggaac aaataggatg
     2101 gatgacacat aatccaccta tcccagtagg agaaatctat aaaagatgga taatcctggg
     2161 attaaataaa atagtaagaa tgtatagccc taccagcatt ctggacataa gacaaggacc
     2221 aaaggaaccc tttagagact atgtagaccg attctataaa actctaagag ccgagcaagc
     2281 ttcacaagag gtaaaaaatt ggatgacaga aaccttgttg gtccaaaatg cgaacccaga
     2341 ttgtaagact attttaaaag cattgggacc aggagcgaca ctagaagaaa tgatgacagc
     2401 atgtcaggga gtggggggac ccggccataa agcaagagtt ttggctgaag caatgagcca
     2461 agtaacaaat ccagctacca taatgataca gaaaggcaat tttaggaacc aaagaaagac
     2521 tgttaagtgt ttcaattgtg gcaaagaagg gcacatagcc aaaaattgca gggcccctag
     2581 gaaaaagggc tgttggaaat gtggaaagga aggacaccaa atgaaagatt gtactgagag
     2641 acaggctaat tttttaggga agatctggcc ttcccacaag ggaaggccag ggaattttct
     2701 tcagagcaga ccagagccaa cagccccacc agaagagagc ttcaggtttg gggaagagac
     2761 aacaactccc tctcagaagc aggagccgat agacaaggaa ctgtatcctt tagcttccct
     2821 cagatcactc tttggcagcg acccctcgtc acaataaaga taggggggca attaaaggaa
     2881 gctctattag atacaggagc agatgataca gtattagaag aaatgaattt gccaggaaga
     2941 tggaaaccaa aaatgatagg gggaattgga ggttttatca aagtaagaca gtatgatcag
     3001 atactcatag aaatctgcgg acataaagct ataggtacag tattagtagg acctacacct
     3061 gtcaacataa ttggaagaaa tctgttgact cagattggct gcactttaaa ttttcccatt
     3121 agtcctattg agactgtacc agtaaaatta aagccaggaa tggatggccc aaaagttaaa
     3181 caatggccat tgacagaaga aaaaataaaa gcattagtag aaatttgtac agaaatggaa
     3241 aaggaaggaa aaatttcaaa aattgggcct gaaaatccat acaatactcc agtatttgcc
     3301 ataaagaaaa aagacagtac taaatggaga aaattagtag atttcagaga acttaataag
     3361 agaactcaag atttctggga agttcaatta ggaataccac atcctgcagg gttaaaacag
     3421 aaaaaatcag taacagtact ggatgtgggc gatgcatatt tttcagttcc cttagataaa
     3481 gacttcagga agtatactgc atttaccata cctagtataa acaatgagac accagggatt
     3541 agatatcagt acaatgtgct tccacaggga tggaaaggat caccagcaat attccagtgt
     3601 agcatgacaa aaatcttaga gccttttaga aaacaaaatc cagacatagt catctatcaa
     3661 tacatggatg atttgtatgt aggatctgac ttagaaatag ggcagcatag aacaaaaata
     3721 gaggaactga gacaacatct gttgaggtgg ggatttacca caccagacaa aaaacatcag
     3781 aaagaacctc cattcctttg gatgggttat gaactccatc ctgataaatg gacagtacag
     3841 cctatagtgc tgccagaaaa ggacagctgg actgtcaatg acatacagaa attagtggga
     3901 aaattgaatt gggcaagtca gatttatgca gggattaaag taaggcaatt atgtaaactt
     3961 cttaggggaa ccaaagcact aacagaagta gtaccactaa cagaagaagc agagctagaa
     4021 ctggcagaaa acagggagat tctaaaagaa ccggtacatg gagtgtatta tgacccatca
     4081 aaagacttaa tagcagaaat acagaagcag gggcaaggcc aatggacata tcaaatttat
     4141 caagagccat ttaaaaatct gaaaacagga aagtatgcaa gaatgaaggg tgcccacact
     4201 aatgatgtga aacaattaac agaggcagta caaaaaatag ccacagaaag catagtaata
     4261 tggggaaaga ctcctaaatt taaattaccc atacaaaagg aaacatggga agcatggtgg
     4321 acagagtatt ggcaagccac ctggattcct gagtgggagt ttgtcaatac ccctccctta
     4381 gtgaagttat ggtaccagtt agagaaagaa cccataatag gagcagaaac tttctatgta
     4441 gatggggcag ccaataggga aactaaatta ggaaaagcag gatatgtaac tgacagagga
     4501 agacaaaaag ttgtccccct aacggacaca acaaatcaga agactgagtt acaagcaatt
     4561 catctagctt tgcaggattc gggattagaa gtaaacatag tgacagactc acaatatgca
     4621 ttgggaatca ttcaagcaca accagataag agtgaatcag agttagtcag tcaaataata
     4681 gagcagttaa taaaaaagga aaaagtctac ctggcatggg taccagcaca caaaggaatt
     4741 ggaggaaatg aacaagtaga taaattggtc agtgctggaa tcaggaaagt actattttta
     4801 gatggaatag ataaggccca agaagaacat gagaaatatc acagtaattg gagagcaatg
     4861 gctagtgatt ttaacctacc acctgtagta gcaaaagaaa tagtagccag ctgtgataaa
     4921 tgtcagctaa aaggggaagc catgcatgga caagtagact gtagcccagg aatatggcag
     4981 ctagattgta cacatttaga aggaaaagtt atcttggtag cagttcatgt agccagtgga
     5041 tatatagaag cagaagtaat tccagcagag acagggcaag aaacagcata cttcctctta
     5101 aaattagcag gaagatggcc agtaaaaaca gtacatacag acaatggcag caatttcacc
     5161 agtactacag ttaaggccgc ctgttggtgg gcggggatca agcaggaatt tggcattccc
     5221 tacaatcccc aaagtcaagg agtaatagaa tctatgaata aagaattaaa gaaaattata
     5281 ggacaggtaa gagatcaggc tgaacatctt aagacagcag tacaaatggc agtattcatc
     5341 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     5401 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     5461 tttcgggttt attacaggga cagcagagat ccagtttgga aaggaccagc aaagctcctc
     5521 tggaaaggtg aaggggcagt agtaatacaa gataatagtg acataaaagt agtgccaaga
     5581 agaaaagcaa agatcatcag ggattatgga aaacagatgg caggtgatga ttgtgtggca
     5641 agtagacagg atgaggatta acacatggaa ttccggagcg gccgcaggag ctttgttcct
     5701 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     5761 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     5821 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     5881 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
     5941 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
     6001 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
     6061 cttccgcgga attcacccca ccagtgcagg ctgcctatca gaaagtggtg gctggtgtgg
     6121 ctaatgccct ggcccacaag tatcactaag ctcgctttct tgctgtccaa tttctattaa
     6181 aggttccttt gttccctaag tccaactact aaactggggg atattatgaa gggccttgag
     6241 catctggatt ctgcctaata aaaaacattt attttcattg caatgatgta tttaaattat
     6301 ttctgaatat tttactaaaa agggaatgtg ggaggtcagt gcatttaaaa cataaagaaa
     6361 tgaagagcta gttcaaacct tgggaaaata cactatatct taaactccat gaaagaaggt
     6421 gaggctgcaa acagctaatg cacattggca acagcccctg atgcctatgc cttattcatc
     6481 cctcagaaaa ggattcaagt agaggcttga tttggaggtt aaagttttgc tatgctgtat
     6541 tttacattac ttattgtttt agctgtcctc atgaatgtct tttcactacc catttgctta
     6601 tcctgcatct ctcagccttg actccactca gttctcttgc ttagagatac cacctttccc
     6661 ctgaagtgtt ccttccatgt tttacggcga gatggtttct cctcgcctgg ccactcagcc
     6721 ttagttgtct ctgttgtctt atagaggtct acttgaagaa ggaaaaacag ggggcatggt
     6781 ttgactgtcc tgtgagccct tcttccctgc ctcccccact cacagtgacc cggaatccct
     6841 cgacatggca gtctagcact agtgcggccg cagatctgct tcctcgctca ctgactcgct
     6901 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     6961 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     7021 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     7081 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     7141 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     7201 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     7261 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     7321 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     7381 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     7441 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     7501 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     7561 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     7621 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     7681 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     7741 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     7801 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     7861 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     7921 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     7981 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     8041 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     8101 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     8161 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     8221 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     8281 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     8341 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     8401 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     8461 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     8521 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     8581 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     8641 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     8701 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     8761 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacggga tcccctgagg
     8821 gggcccccat gggctagagg atccggcctc ggcctctgca taaataaaaa aaattagtca
     8881 gccatgagc
//
