LOCUS       .                       2842 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        19..63
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     primer_bind     121..137
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(207..223)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        complement(249..293)
                     /label="T5 promoter"
                     /note="bacteriophage T5 promoter for E. coli RNA
                     polymerase, with embedded lac operator"
     primer_bind     complement(303..319)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      461..916
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        942..1046
                     /label="AmpR promoter"
     CDS             1047..1904
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      2078..2666
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 aacctggctt atcgaaattc ataaaaaatt tatttgcttt gtgagcggat aacaattata
       61 atagagaccg gcagatctga tatcatcgat gaattcgagc tccaccgcgg tggcggccgc
      121 tctagaacta gtggatccac cggttccatg gctagccacg tgacgcgtgg atcccccggg
      181 ctgcaggaat tcgatatcaa gcttatcgat accgtcgacc tcgagggggg gcccggtacc
      241 caattcgcta ttataattgt tatccgctca caaagcaaat aaatttttta tgacgcgcgc
      301 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      361 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      421 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
      481 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      541 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      601 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      661 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
      721 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
      781 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
      841 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
      901 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1021 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1081 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1141 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1201 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1261 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1321 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1381 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1441 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1501 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1561 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1621 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1681 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1741 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1801 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1861 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1921 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2221 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2701 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2761 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2821 gcgagtcagt gagcgaggaa gc
//
