LOCUS       .                      17220 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     misc_feature    complement(21..45)
                     /label="RB T-DNA repeat"
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             977..1765
                     /label="SmR"
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
     misc_RNA        1920..1995
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        2282..2953
                     /label="CaMV 35S promoter (enhanced)"
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             3223..7323
                     /label="Cas9"
                     /note="S. pyogenes Cas9, codon optimized for C. elegans
                     with synthetic introns"
     terminator      8164..8796
                     /label="E9 terminator"
                     /note="terminator and polyadenylation signal from the pea
                     rbcS-E9 gene"
     promoter        complement(8859..8889)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8904..8925)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        9441..9786
                     /label="CaMV 35S promoter"
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             9861..10649
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    10709..10883
                     /label="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(10961..10985)
                     /label="LB T-DNA repeat"
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             11410..12201
                     /label="KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      12291..12879
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(13065..13205)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(13549..13743)
                     /label="pVS1 oriV"
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(13812..14882)
                     /label="pVS1 RepA"
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(15314..15940)
                     /label="pVS1 StaA"
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgccaag cttcgacttg ccttccgcac aatacatcat
      301 ttcttcttag ctttttttct tcttcttcgt tcatacagtt tttttttgtt tatcagctta
      361 cattttcttg aaccgtagct ttcgttttct tctttttaac tttccattcg gagtttttgt
      421 atcttgtttc atagtttgtc ccaggattag aatgattagg catcgaacct tcaagaattt
      481 gattgaataa aacatcttca ttcttaagat atgaagataa tcttcaaaag gcccctggga
      541 atctgaaaga agagaagcag gcccatttat atgggaaaga acaatagtat ttcttatata
      601 ggcccattta agttgaaaac aatcttcaaa agtcccacat cgcttagata agaaaacgaa
      661 gctgagttta tatacagcta gagtcgaagt agtgattggg agaccaaccc agtggacata
      721 agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag
      781 aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga
      841 ctgttttttt ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt
      901 gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa
      961 aacaaagtta aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga
     1021 ggtagttggc gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg
     1081 ctccgcagtg gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac
     1141 cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc
     1201 ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga
     1261 catcattccg tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa
     1321 tgacattctt gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct
     1381 gacaaaagca agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga
     1441 tccggttcct gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc
     1501 gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta
     1561 cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg
     1621 cctgccggcc cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga
     1681 agaagatcgc ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg
     1741 cgagatcacc aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc
     1801 gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc
     1861 aaactatcag gtcaagtctg cttttattat ttttaagcgt gcataataag ccggtctcgg
     1921 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     1981 gcaccgagtc ggtgcttttt tttgcaaaat tttccagatc gatttcttct tcctctgttc
     2041 ttcggcgttc aatttctggg gttttctctt cgttttctgt aactgaaacc taaaatttga
     2101 cctaaaaaaa atctcaaata atatgattca gtggttttgt acttttcagt tagttgagtt
     2161 ttgcagttcc gatgagataa accaataagc ttgcatgcct gcaggtcaac atggtggagc
     2221 acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac caaagggcaa
     2281 ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat tgcccagcta
     2341 tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa tgccatcatt
     2401 gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc aaagatggac
     2461 ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct tcaaagcaag
     2521 tggattgatg tgataacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag
     2581 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
     2641 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
     2701 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
     2761 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
     2821 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
     2881 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
     2941 atttggagag gacctcgacc tcaacacaac atatacaaaa caaacgaatc tcaagcaatc
     3001 aagcattcta cttctattgc agcaatttaa atcatttctt ttaaagcaaa agcaattttc
     3061 tgaaaatttt caccatttac gaacgatact cgagtaatct agatggatta caaggaccac
     3121 gacggggatt acaaggacca cgacattgat tacaaggatg atgatgacaa gatggctccg
     3181 aagaagaaga ggaaggttgg catccacggg gtgccagctg ctgacaagaa gtactcgatc
     3241 ggcctcgata ttgggactaa ctctgttggc tgggccgtga tcaccgacga gtacaaggtg
     3301 ccctcaaaga agttcaaggt cctgggcaac accgatcggc attccatcaa gaagaatctc
     3361 attggcgctc tcctgttcga cagcggcgag acggctgagg ctacgcggct caagcgcacc
     3421 gcccgcaggc ggtacacgcg caggaagaat cgcatctgct acctgcagga gattttctcc
     3481 aacgagatgg cgaaggttga cgattctttc ttccacaggc tggaggagtc attcctcgtg
     3541 gaggaggata agaagcacga gcggcatcca atcttcggca acattgtcga cgaggttgcc
     3601 taccacgaga agtaccctac gatctaccat ctgcggaaga agctcgtgga ctccacagat
     3661 aaggcggacc tccgcctgat ctacctcgct ctggcccaca tgattaagtt caggggccat
     3721 ttcctgatcg agggggatct caacccggac aatagcgatg ttgacaagct gttcatccag
     3781 ctcgtgcaga cgtacaacca gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac
     3841 gcgaaggcta tcctgtccgc taggctctcg aagtctcggc gcctcgagaa cctgatcgcc
     3901 cagctgccgg gcgagaagaa gaacggcctg ttcgggaatc tcattgcgct cagcctgggg
     3961 ctcacgccca acttcaagtc gaatttcgat ctcgctgagg acgccaagct gcagctctcc
     4021 aaggacacat acgacgatga cctggataac ctcctggccc agatcggcga tcagtacgcg
     4081 gacctgttcc tcgctgccaa gaatctgtcg gacgccatcc tcctgtctga tattctcagg
     4141 gtgaacaccg agattacgaa ggctccgctc tcagcctcca tgatcaagcg ctacgacgag
     4201 caccatcagg atctgaccct cctgaaggcg ctggtcaggc agcagctccc cgagaagtac
     4261 aaggagatct tcttcgatca gtcgaagaac ggctacgctg ggtacattga cggcggggcc
     4321 tctcaggagg agttctacaa gttcatcaag ccgattctgg agaagatgga cggcacggag
     4381 gagctgctgg tgaagctcaa tcgcgaggac ctcctgagga agcagcggac attcgataac
     4441 ggcagcatcc cacaccagat tcatctcggg gagctgcacg ctatcctgag gaggcaggag
     4501 gacttctacc ctttcctcaa ggataaccgc gagaagatcg agaagattct gactttcagg
     4561 atcccgtact acgtcggccc actcgctagg ggcaactccc gcttcgcttg gatgacccgc
     4621 aagtcagagg agacgatcac gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc
     4681 gctcagtcgt tcatcgagag gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg
     4741 ctccctaagc actcgctcct gtacgagtac ttcacagtct acaacgagct gactaaggtg
     4801 aagtatgtga ccgagggcat gaggaagccg gctttcctgt ctggggagca gaagaaggcc
     4861 atcgtggacc tcctgttcaa gaccaaccgg aaggtcacgg ttaagcagct caaggaggac
     4921 tacttcaaga agattgagtg cttcgattcg gtcgagatct ctggcgttga ggaccgcttc
     4981 aacgcctccc tggggaccta ccacgatctc ctgaagatca ttaaggataa ggacttcctg
     5041 gacaacgagg agaatgagga tatcctcgag gacattgtgc tgacactcac tctgttcgag
     5101 gaccgggaga tgatcgagga gcgcctgaag acttacgccc atctcttcga tgacaaggtc
     5161 atgaagcagc tcaagaggag gaggtacacc ggctggggga ggctgagcag gaagctcatc
     5221 aacggcattc gggacaagca gtccgggaag acgatcctcg acttcctgaa gagcgatggc
     5281 ttcgcgaacc gcaatttcat gcagctgatt cacgatgaca gcctcacatt caaggaggat
     5341 atccagaagg ctcaggtgag cggccagggg gactcgctgc acgagcatat cgcgaacctc
     5401 gctggctcgc cagctatcaa gaaggggatt ctgcagaccg tgaaggttgt ggacgagctg
     5461 gtgaaggtca tgggcaggca caagcctgag aacatcgtca ttgagatggc ccgggagaat
     5521 cagaccacgc agaagggcca gaagaactca cgcgagagga tgaagaggat cgaggagggc
     5581 attaaggagc tggggtccca gatcctcaag gagcacccgg tggagaacac gcagctgcag
     5641 aatgagaagc tctacctgta ctacctccag aatggccgcg atatgtatgt ggaccaggag
     5701 ctggatatta acaggctcag cgattacgac gtcgatcata tcgttccaca gtcattcctg
     5761 aaggatgact ccattgacaa caaggtcctc accaggtcgg acaagaaccg gggcaagtct
     5821 gataatgttc cttcagagga ggtcgttaag aagatgaaga actactggcg ccagctcctg
     5881 aatgccaagc tgatcacgca gcggaagttc gataacctca caaaggctga gaggggcggg
     5941 ctctctgagc tggacaaggc gggcttcatc aagaggcagc tggtcgagac acggcagatc
     6001 actaagcacg ttgcgcagat tctcgactca cggatgaaca ctaagtacga tgagaatgac
     6061 aagctgatcc gcgaggtgaa ggtcatcacc ctgaagtcaa agctcgtctc cgacttcagg
     6121 aaggatttcc agttctacaa ggttcgggag atcaacaatt accaccatgc ccatgacgcg
     6181 tacctgaacg cggtggtcgg cacagctctg atcaagaagt acccaaagct cgagagcgag
     6241 ttcgtgtacg gggactacaa ggtttacgat gtgaggaaga tgatcgccaa gtcggagcag
     6301 gagattggca aggctaccgc caagtacttc ttctactcta acattatgaa tttcttcaag
     6361 acagagatca ctctggccaa tggcgagatc cggaagcgcc ccctcatcga gacgaacggc
     6421 gagacggggg agatcgtgtg ggacaagggc agggatttcg cgaccgtcag gaaggttctc
     6481 tccatgccac aagtgaatat cgtcaagaag acagaggtcc agactggcgg gttctctaag
     6541 gagtcaattc tgcctaagcg gaacagcgac aagctcatcg cccgcaagaa ggactgggat
     6601 ccgaagaagt acggcgggtt cgacagcccc actgtggcct actcggtcct ggttgtggcg
     6661 aaggttgaga agggcaagtc caagaagctc aagagcgtga aggagctgct ggggatcacg
     6721 attatggagc gctccagctt cgagaagaac ccgatcgatt tcctggaggc gaagggctac
     6781 aaggaggtga agaaggacct gatcattaag ctccccaagt actcactctt cgagctggag
     6841 aacggcagga agcggatgct ggcttccgct ggcgagctgc agaaggggaa cgagctggct
     6901 ctgccgtcca agtatgtgaa cttcctctac ctggcctccc actacgagaa gctcaagggc
     6961 agccccgagg acaacgagca gaagcagctg ttcgtcgagc agcacaagca ttacctcgac
     7021 gagatcattg agcagatttc cgagttctcc aagcgcgtga tcctggccga cgcgaatctg
     7081 gataaggtcc tctccgcgta caacaagcac cgcgacaagc caatcaggga gcaggctgag
     7141 aatatcattc atctcttcac cctgacgaac ctcggcgccc ctgctgcttt caagtacttc
     7201 gacacaacta tcgatcgcaa gaggtacaca agcactaagg aggtcctgga cgcgaccctc
     7261 atccaccagt cgattaccgg cctctacgag acgcgcatcg acctgtctca gctcgggggc
     7321 gacaagcggc cagcggcgac gaagaaggcg gggcaggcga agaagaagaa gggatccggc
     7381 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg
     7441 gtgtctaagg gcgaagagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     7501 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     7561 aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     7621 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     7681 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     7741 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     7801 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     7861 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     7921 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     7981 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     8041 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     8101 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtgagct
     8161 cagagctttc gttcgtatca tcggtttcga caacgttcgt caagttcaat gcatcagttt
     8221 cattgcgcac acaccagaat cctactgagt ttgagtatta tggcattggg aaaactgttt
     8281 ttcttgtacc atttgttgtg cttgtaattt actgtgtttt ttattcggtt ttcgctatcg
     8341 aactgtgaaa tggaaatgga tggagaagag ttaatgaatg atatggtcct tttgttcatt
     8401 ctcaaattaa tattatttgt tttttctctt atttgttgtg tgttgaattt gaaattataa
     8461 gagatatgca aacattttgt tttgagtaaa aatgtgtcaa atcgtggcct ctaatgaccg
     8521 aagttaatat gaggagtaaa acacttgtag ttgtaccatt atgcttattc actaggcaac
     8581 aaatatattt tcagacctag aaaagctgca aatgttactg aatacaagta tgtcctcttg
     8641 tgttttagac atttatgaac tttcctttat gtaattttcc agaatccttg tcagattcta
     8701 atcattgctt tataattata gttatactca tggatttgta gttgagtatg aaaatatttt
     8761 ttaatgcatt ttatgacttg ccaattgatt gacaacgaat tcgtaatcat gtcatagctg
     8821 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     8881 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     8941 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     9001 gcggggagag gcggtttgcg tattggctag agcagcttgc caacatggtg gagcacgaca
     9061 ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg gctattgaga
     9121 cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca gctatctgtc
     9181 acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat cattgcgata
     9241 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
     9301 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
     9361 gatgtgaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc
     9421 tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc
     9481 ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc
     9541 acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac
     9601 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
     9661 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
     9721 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
     9781 aggacacgct gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga
     9841 tctgtcgatc gaccatgggg attgaacaag atggattgca cgcaggttct ccggccgctt
     9901 gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg
     9961 ccgtgtttcg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg
    10021 gtgccctgaa tgaactccag gacgaggcag cgcggctatc gtggctggcc acgacgggcg
    10081 ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg
    10141 gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca
    10201 tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc
    10261 accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc
    10321 aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca
    10381 aggcgcgcat gcccgacggc gaggatctcg tcgtgacaca tggcgatgcc tgcttgccga
    10441 atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg
    10501 cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg
    10561 aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg
    10621 ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg gatcgatcct
    10681 ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag tagttcccag
    10741 ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata agaaaccctt
    10801 agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc ctaaaaccaa
    10861 aatccagtac taaaatccag atcccccgaa ttaattcggc gttaattcag tacattaaaa
    10921 acgtccgcaa tgtgttatta agttgtctaa gcgtcaattt gtttacacca caatatatcc
    10981 tgccaccagc cagccaacag ctccccgacc ggcagctcgg cacaaaatca ccactcgata
    11041 caggcagccc atcagtccgg gacggcgtca gcgggagagc cgttgtaagg cggcagactt
    11101 tgctcatgtt accgatgcta ttcggaagaa cggcaactaa gctgccgggt ttgaaacacg
    11161 gatgatctcg cggagggtag catgttgatt gtaacgatga cagagcgttg ctgcctgtga
    11221 tcaccgcggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
    11281 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
    11341 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
    11401 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
    11461 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
    11521 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
    11581 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
    11641 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
    11701 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
    11761 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
    11821 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
    11881 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
    11941 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
    12001 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
    12061 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
    12121 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
    12181 attttactgg atgaattgtt ttagtaccta gaatgcatga ccaaaatccc ttaacgtgag
    12241 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
    12301 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
    12361 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
    12421 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
    12481 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
    12541 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
    12601 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
    12661 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
    12721 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
    12781 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
    12841 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
    12901 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
    12961 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
    13021 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
    13081 ctccttacgc atctgtgcgg tatttcacac cgcatatggt gcactctcag tacaatctgc
    13141 tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg
    13201 ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
    13261 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
    13321 cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg ggcgccggcg gtcgagtggc
    13381 gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc gtaggccagc catttttgag
    13441 cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc gtagggagcg cagcgaccga
    13501 agggtaggcg ctttttgcag ctcttcggct gtgcgctggc cagacagtta tgcacaggcc
    13561 aggcgggttt taagagtttt aataagtttt aaagagtttt aggcggaaaa atcgcctttt
    13621 ttctctttta tatcagtcac ttacatgtgt gaccggttcc caatgtacgg ctttgggttc
    13681 ccaatgtacg ggttccggtt cccaatgtac ggctttgggt tcccaatgta cgtgctatcc
    13741 acaggaaaca gaccttttcg acctttttcc cctgctaggg caatttgccc tagcatctgc
    13801 tccgtacatt aggaaccggc ggatgcttcg ccctcgatca ggttgcggta gcgcatgact
    13861 aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt actccggcag gtcatttgac
    13921 ccgatcagct tgcgcacggt gaaacagaac ttcttgaact ctccggcgct gccactgcgt
    13981 tcgtagatcg tcttgaacaa ccatctggct tctgccttgc ctgcggcgcg gcgtgccagg
    14041 cggtagagaa aacggccgat gccgggatcg atcaaaaagt aatcggggtg aaccgtcagc
    14101 acgtccgggt tcttgccttc tgtgatctcg cggtacatcc aatcagctag ctcgatctcg
    14161 atgtactccg gccgcccggt ttcgctcttt acgatcttgt agcggctaat caaggcttca
    14221 ccctcggata ccgtcaccag gcggccgttc ttggccttct tcgtacgctg catggcaacg
    14281 tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt ctttctgctt tccgccatcg
    14341 gctcgccggc agaacttgag tacgtccgca acgtgtggac ggaacacgcg gccgggcttg
    14401 tctcccttcc cttcccggta tcggttcatg gattcggtta gatgggaaac cgccatcagt
    14461 accaggtcgt aatcccacac actggccatg ccggccggcc ctgcggaaac ctctacgtgc
    14521 ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc ggtcacgctt cgacagacgg
    14581 aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc ccacgtcata gagcatcgga
    14641 acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc taatcgacgg cgcaccggct
    14701 gccggcggtt gccgggattc tttgcggatt cgatcagcgg ccgcttgcca cgattcaccg
    14761 gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct gcgcggcctt caacttctcc
    14821 accaggtcat cacccagcgc cgcgccgatt tgtaccgggc cggatggttt gcgaccgctc
    14881 acgccgattc ctcgggcttg ggggttccag tgccattgca gggccggcag gcaacccagc
    14941 cgcttacgcc tggccaaccg cccgttcctc cacacatggg gcattccacg gcgtcggtgc
    15001 ctggttgttc ttgattttcc atgccgcctc ctttagccgc taaaattcat ctactcattt
    15061 attcatttgc tcatttactc tggtagctgc gcgatgtatt cagatagcag ctcggtaatg
    15121 gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt gatcctccgc cggcaactga
    15181 aagttgaccc gcttcatggc tggcgtgtct gccaggctgg ccaacgttgc agccttgctg
    15241 ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc ttttgctcat tttctcttta
    15301 cctcattaac tcaaatgagt tttgatttaa tttcagcggc cagcgcctgg acctcgcggg
    15361 cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc ggcggcggca gtgcctgggt
    15421 agctcacgcg ctgcgtgata cgggactcaa gaatgggcag ctcgtacccg gccagcgcct
    15481 cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg cgacacgaca aaggccgctt
    15541 gtagccttcc atccgtgacc tcaatgcgct gcttaaccag ctccaccagg tcggcggtgg
    15601 cccatatgtc gtaagggctt ggctgcaccg gaatcagcac gaagtcggct gccttgatcg
    15661 cggacacagc caagtccgcc gcctggggcg ctccgtcgat cactacgaag tcgcgccggc
    15721 cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat gccgacaacg gttagcggtt
    15781 gatcttcccg cacggccgcc caatcgcggg cactgccctg gggatcggaa tcgactaaca
    15841 gaacatcggc cccggcgagt tgcagggcgc gggctagatg ggttgcgatg gtcgtcttgc
    15901 ctgacccgcc tttctggtta agtacagcga taaccttcat gcgttcccct tgcgtatttg
    15961 tttatttact catcgcatca tatacgcagc gaccgcatga cgcaagctgt tttactcaaa
    16021 tacacatcac ctttttagac ggcggcgctc ggtttcttca gcggccaagc tggccggcca
    16081 ggccgccagc ttggcatcag acaaaccggc caggatttca tgcagccgca cggttgagac
    16141 gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc tccgcctcga tctcttcggt
    16201 aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc atgcttgttc ctcttggcgt
    16261 tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg cgtcctcacg gaaggcaccg
    16321 cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct caagtgcgcg gtacagggtc
    16381 gagcgatgca cgccaagcag tgcagccgcc tctttcacgg tgcggccttc ctggtcgatc
    16441 agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag ggcgggggcc aaacttcacg
    16501 cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt cgatgattag ggaacgctcg
    16561 aactcggcaa tgccggcgaa cacggtcaac accatgcggc cggccggcgt ggtggtgtcg
    16621 gcccacggct ctgccaggct acgcaggccc gcgccggcct cctggatgcg ctcggcaatg
    16681 tccagtaggt cgcgggtgct gcgggccagg cggtctagcc tggtcactgt cacaacgtcg
    16741 ccagggcgta ggtggtcaag catcctggcc agctccgggc ggtcgcgcct ggtgccggtg
    16801 atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca gttcggcccg ttggttggtc
    16861 aagtcctggt cgtcggtgct gacgcgggca tagcccagca ggccagcggc ggcgctcttg
    16921 ttcatggcgt aatgtctccg gttctagtcg caagtattct actttatgcg actaaaacac
    16981 gcgacaagaa aacgccagga aaagggcagg gcggcagcct gtcgcgtaac ttaggacttg
    17041 tgcgacatgt cgttttcaga agacggctgc actgaacgtc agaagccgac tgcactatag
    17101 cagcggaggg gttggatcaa agtactttga tcccgagggg aaccctgtgg ttggcatgca
    17161 catacaaatg gacgaacgga taaacctttt cacgcccttt taaatatccg attattctaa
//
