LOCUS       .                       3601 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        458..562
                     /label="AmpR promoter"
     CDS             563..1585
                     /label="HygR"
                     /note="hygromycin B phosphotransferase"
     protein_bind    complement(1779..1812)
                     /label="FRT (minimal)"
                     /note="supports FLP-mediated excision but not integration
                     (Turan and Bode, 2011)"
     rep_origin      complement(1861..2244)
                     /label="R6K γ ori"
                     /note="γ replication origin from E. coli plasmid R6K;
                     requires the R6K initiator protein pi for replication"
     CDS             complement(2317..3174)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(3175..3279)
                     /label="AmpR promoter"
     terminator      3369..3455
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      3547..3574
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 agattgcagc attacacgtc ttgagcgatt gtgtaggctg gagctgcttc gaagttccta
       61 tactttctag agaataggaa cttcggaata ggaacttcaa gatcccccac gctgccgcaa
      121 gcactcaggg cgcaagggct gctaaaggaa gcggaacacg tagaaagcca gtccgcagaa
      181 acggtgctga ccccggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgccgc ggaaccccta tttgtttatt
      481 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      541 ataatattga aaaaggaaga gtatgaaaaa gcctgaactc accgcgacgt ctgtcgagaa
      601 gtttctgatc gaaaagttcg acagcgtctc cgacctgatg cagctctcgg agggcgaaga
      661 atctcgtgct ttcagcttcg atgtaggagg gcgtggatat gtcctgcggg taaatagctg
      721 cgccgatggt ttctacaaag atcgttatgt ttatcggcac tttgcatcgg ccgcgctccc
      781 gattccggaa gtgcttgaca ttggggaatt cagcgagagc ctgacctatt gcatctcccg
      841 ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc gaactgcccg ctgttctgca
      901 gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat cttagccaga cgagcgggtt
      961 cggcccattc ggaccgcaag gaatcggtca atacactaca tggcgtgatt tcatatgcgc
     1021 gattgctgat ccccatgtgt atcactggca aactgtgatg gacgacaccg tcagtgcgtc
     1081 cgtcgcgcag gctctcgatg agctgatgct ttgggccgag gactgccccg aagtccggca
     1141 cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg gacaatggcc gcataacagc
     1201 ggtcattgac tggagcgagg cgatgttcgg ggattcccaa tacgaggtcg ccaacatctt
     1261 cttctggagg ccgtggttgg cttgtatgga gcagcagacg cgctacttcg agcggaggca
     1321 tccggagctt gcaggatcgc cgcggctccg ggcgtatatg ctccgcattg gtcttgacca
     1381 actctatcag agcttggttg acggcaattt cgatgatgca gcttgggcgc agggtcgatg
     1441 cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt acacaaatcg cccgcagaag
     1501 cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc gatagtggaa accgacgccc
     1561 cagcactcgt ccgagggcaa aggaataggc gggactctgg ggttcgaaat gaccgaccaa
     1621 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     1681 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     1741 ctggagttct tcgcccaccc cagcttcaaa agcgctctga agttcctata ctttctagag
     1801 aataggaact tcggaatagg aactaaggag gatattcata tggaccatgg ctaattccca
     1861 tgtcagccgt taagtgttcc tgtgtcactc aaaattgctt tgagaggctc taagggcttc
     1921 tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag
     1981 ccttatatat tctttttttt cttataaaac ttaaaacctt agaggctatt taagttgctg
     2041 atttatatta attttattgt tcaaacatga gagcttagta cgtgaaacat gagagcttag
     2101 tacgttagcc atgagagctt agtacgttag ccatgagggt ttagttcgtt aaacatgaga
     2161 gcttagtacg ttaaacatga gagcttagta cgtgaaacat gagagcttag tacgtactat
     2221 caacaggttg aactgcggat cttgcggccg caaaaattaa aaatgaagtt ttaaatcaat
     2281 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2341 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2401 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2461 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2521 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2581 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2641 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2701 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2761 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     2821 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     2881 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     2941 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3001 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3061 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3121 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3181 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3241 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3301 acctgcatcg atggcccccg atggtagtgt ggggtctccc catgcgagag tagggaactg
     3361 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     3421 gtttgtcggt gaacgctctc ctgagtagga caaatccgcc gggagcggat ttgaacgttg
     3481 cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa
     3541 ttaagcagaa ggccatcctg acggatggcc tttttgcgtg gccagtgcca agcttgcatg
     3601 c
//
