LOCUS       .                       4068 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    42..63
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        78..108
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    116..132
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     primer_bind     140..156
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             179..196
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             197..622
                     /label="protein A"
                     /note="immunoglobulin binding domains of Staphylocuccus
                     aureus protein A"
     CDS             641..1597
                     /label="TurboID"
                     /note="mutant of the E. coli biotin ligase BioID engineered
                     for improved catalytic efficiency (Branon et al., 2018)"
     primer_bind     complement(1635..1651)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             2156..2947
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     rep_origin      3234..3822
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
       61 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
      121 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttcatgca
      181 tcatcaccac caccatagct taaaagatga cccaagccaa agtgctaacc tattgtcaga
      241 agctaaaaag ttaaatgaat ctcaagcacc gaaagcggat aacaaattca acaaagaaca
      301 acaaaatgct ttctatgaaa tcttacattt acctaactta aacgaagaac aacgcaatgg
      361 tttcatccaa agcctaaaag atgacccaag ccaaagcgct aaccttttag cagaagctaa
      421 aaagctaaat gatgctcaag caccaaaagc tgacaacaaa ttcaacaaag aacaacaaaa
      481 tgctttctat gaaattttac atttacctaa cttaactgaa gaacaacgta acggcttcat
      541 ccaaagcctt aaagacgatc cttcagtgag caaagaaatt ttagcagaag ctaaaaagct
      601 aaacgatgct caagcaccaa aagatgacga taaagaattc aaagacaata ctgtgcctct
      661 gaagctgatc gctctcctgg ctaatggcga gttccatagt ggcgaacagc tgggagaaac
      721 cctgggcatg tccagggccg ctatcaacaa gcacattcag actctgcgcg actggggcgt
      781 ggacgtgttc accgtgcccg gaaagggcta ctctctgccc gagcctatcc cgctgctgaa
      841 cgctaaacag attctgggac agctggacgg cgggagcgtg gcagtcctgc ctgtggtcga
      901 ctccaccaat cagtacctgc tggatcgaat cggcgagctg aagagtgggg atgcttgcat
      961 tgcagaatat cagcaggcag ggagaggaag cagagggagg aaatggttct ctccttttgg
     1021 agctaacctg tacctgagta tgttttggcg cctgaagcgg ggaccagcag caatcggcct
     1081 gggcccggtc atcggaattg tcatggcaga agcgctgcga aagctgggag cagacaaggt
     1141 gcgagtcaaa tggcccaatg acctgtatct gcaggataga aagctggcag gcatcctggt
     1201 ggagctggcc ggaataacag gcgatgctgc acagatcgtc attggcgccg ggattaacgt
     1261 ggctatgagg cgcgtggagg aaagcgtggt caatcagggc tggatcacac tgcaggaagc
     1321 agggattaac ctggacagga atactctggc cgctacgctg atccgagagc tgcgggcagc
     1381 cctggaactg ttcgagcagg aaggcctggc tccatatctg ccacggtggg agaagctgga
     1441 taacttcatc aatagacccg tgaagctgat cattggggac aaagagattt tcgggattag
     1501 ccgggggatt gataaacagg gagccctgct gctggaacag gacggagtta tcaaaccctg
     1561 gatgggcgga gaaatcagtc tgcggtctgc cgaaaagtaa tgaggatccc cgggtaccga
     1621 gctcgaatta attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     1681 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     1741 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgataagct
     1801 agcttcacgc tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta
     1861 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
     1921 gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg
     1981 atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc
     2041 ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat
     2101 ctgatggcgc aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat
     2161 tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta
     2221 tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca
     2281 ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg aactccaaga
     2341 cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga
     2401 cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct
     2461 cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg
     2521 gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga
     2581 gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca
     2641 tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgcggatgc ccgacggcga
     2701 ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg
     2761 cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc
     2821 gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt
     2881 gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga
     2941 gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca
     3001 tcacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     3061 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     3121 cccaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     3181 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3241 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3301 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3361 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     3421 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3481 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3541 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3601 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     3661 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3721 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3781 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3841 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3901 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3961 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     4021 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgac
//
