LOCUS       .                       3282 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             join(2910..3282,1..419)
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     CDS             429..1847
                     /label="SacB"
                     /note="secreted levansucrase that renders bacterial growth
                     sensitive to sucrose"
     oriT            complement(1867..1975)
                     /label="oriT"
                     /note="incP origin of transfer"
     rep_origin      2127..2715
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     2727..2743
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    2752..2808
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2812..2828)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
       61 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
      121 tcgcgccagc cgaactgttc gccaggctca aggcgcggat gcccgacggc gaggatctcg
      181 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
      241 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
      301 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
      361 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
      421 gagcgacgat gaacatcaaa aagtttgcaa aacaagcaac agtattaacc tttactaccg
      481 cactgctggc aggaggcgca actcaagcgt ttgcgaaaga aacgaaccaa aagccatata
      541 aggaaacata cggcatttcc catattacac gccatgatat gctgcaaatc cctgaacagc
      601 aaaaaaatga aaaatatcaa gtttctgaat ttgattcgtc cacaattaaa aatatctctt
      661 ctgcaaaagg cctggacgtt tgggacagct ggccattaca aaacgctgac ggcactgtcg
      721 caaactatca cggctaccac atcgtctttg cattagccgg agatcctaaa aatgcggatg
      781 acacatcgat ttacatgttc tatcaaaaag tcggcgaaac ttctattgac agctggaaaa
      841 acgctggccg cgtctttaaa gacagcgaca aattcgatgc aaatgattct atcctaaaag
      901 accaaacaca agaatggtca ggttcagcca catttacatc tgacggaaaa atccgtttat
      961 tctacactga tttctccggt aaacattacg gcaaacaaac actgacaact gcacaagtta
     1021 acgtatcagc atcagacagc tctttgaaca tcaacggtgt agaggattat aaatcaatct
     1081 ttgacggtga cggaaaaacg tatcaaaatg tacagcagtt catcgatgaa ggcaactaca
     1141 gctcaggcga caaccatacg ctgagagatc ctcactacgt agaagataaa ggccacaaat
     1201 acttagtatt tgaagcaaac actggaactg aagatggcta ccaaggcgaa gaatctttat
     1261 ttaacaaagc atactatggc aaaagcacat cattcttccg tcaagaaagt caaaaacttc
     1321 tgcaaagcga taaaaaacgc acggctgagt tagcaaacgg cgctctcggt atgattgagc
     1381 taaacgatga ttacacactg aaaaaagtga tgaaaccgct gattgcatct aacacagtaa
     1441 cagatgaaat tgaacgcgcg aacgtcttta aaatgaacgg caaatggtac ctgttcactg
     1501 actcccgcgg atcaaaaatg acgattgacg gcattacgtc taacgatatt tacatgcttg
     1561 gttatgtttc taattcttta actggcccat acaagccgct gaacaaaact ggccttgtgt
     1621 taaaaatgga tcttgatcct aacgatgtaa cctttactta ctcacacttc gctgtacctc
     1681 aagcgaaagg aaacaatgtc gtgattacaa gctatatgac aaacagagga ttctacgcag
     1741 acaaacaatc aacgtttgcg ccgagcttcc tgctgaacat caaaggcaag aaaacatctg
     1801 ttgtcaaaga cagcatcctt gaacaaggac aattaacagt taacaaataa tcagaccccg
     1861 tagaaacggc cagcctcgca gagcaggatt cccgttgagc accgccaggt gcgaataagg
     1921 gacagtgaag aaggaacacc cgctcgcggg tgggcctact tcacctatcc tgcccggctg
     1981 acgccgttgg atacaccaag gaaagtctac acgaaccctt tggcaaaatc ctgtatatcg
     2041 tgcgaaaaag gatggatata ccgaaaaaat cgctataatg accccgaagc agggttatgc
     2101 agcggaaaag agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     2161 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     2221 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     2281 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     2341 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     2401 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     2461 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     2521 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     2581 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     2641 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     2701 cggagcctat ggaaaaacgc ctcacacagg aaacagctat gacatgatta cgaattcgag
     2761 ctcggtaccc ggggatcctc tagagtcgac ctgcaggcat gcaagcttgg cactggccgt
     2821 cgttttacaa cgtcgtgacc ggaattgcca gctggggcgc cctctggtaa ggttgggaag
     2881 ccctgcaaac aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt
     2941 ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct
     3001 gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga
     3061 ccgacctgtc cggtgccctg aatgaactcc aagacgaggc agcgcggcta tcgtggctgg
     3121 ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact
     3181 ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg
     3241 agaaagtatc catcatggct gatgcaatgc ggcggctgca ta
//
