LOCUS       40924_26321        6055 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector pJN105, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Newman JR, Fuqua C.
  TITLE     Broad-host-range expression vectors that carry the 
            L-arabinose-inducible Escherichia coli araBAD promoter and the araC 
            regulator
  JOURNAL   Gene 227 (2), 197-203 (1999)
  PUBMED    10023058
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Newman JR, Fuqua C.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-AUG-1999) Biology, Indiana University, JH142, 1001 E. 
            3rd St., Bloomington, IN 47405-6801, USA
REFERENCE   3  (bases 1 to 6055)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6055)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Gene"; 
            date: "1999"; volume: "227"; issue: "2"; pages: "197-203"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-AUG-1999) Biology, Indiana University, JH142, 1001 E. 3rd St., 
            Bloomington, IN 47405-6801, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6055
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(264..794)
                     /codon_start=1
                     /label=GmR
                     /note="gentamycin acetyltransferase"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(983..1011)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    1281..1302
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1317..1347
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1355..1371
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1379..1395
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1416..1434
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1464..1480
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(1575..2450)
                     /codon_start=1
                     /label=araC
                     /note="L-arabinose regulatory protein"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        2477..2761
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     complement(2801..2817)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2850..2868)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2878..2894)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(3604..4263)
                     /codon_start=1
                     /label=pBBR1 Rep
                     /note="replication protein for the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     rep_origin      complement(4264..5033)
                     /direction=LEFT
                     /label=pBBR1 oriV
                     /note="replication origin of the broad-host-range plasmid
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctggcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
      241 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
      301 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
      361 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag attgatgagc
      421 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
      481 tcactacgcg gctgctcaaa cctgggcaga acgtaagccg cgagagcgcc aacaaccgct
      541 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
      601 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
      661 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
      721 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
      781 ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg cgtaacgcgc
      841 ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac aagccatgaa
      901 aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc agttgcgtga
      961 gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca actgggttcg
     1021 tgccttcatc cgtttccacg gtgtgcgtcc atgggcaaat attatacgca aggcgacaag
     1081 gtgctgatgc cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc
     1141 agaatgctta atgaattaca acagttttta tgcatgcgcc caatacgcaa accgcctctc
     1201 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     1261 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     1321 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     1381 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     1441 aagctgggta ccgggccccc cctcgaggtc gacggtatcg atgcataatg tgcctgtcaa
     1501 atggacgaag cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga
     1561 ttcgttacca attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca
     1621 cggaactcgc tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga
     1681 tcgtcaaaac caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc
     1741 agcttcgcct ggctgatacg ttggtcctcg cgccagctta agacgctaat ccctaactgc
     1801 tggcggaaaa gatgtgacag acgcgacggc gacaagcaaa catgctgtgc gacgctggcg
     1861 atatcaaaat tgctgtctgc caggtgatcg ctgatgtact gacaagcctc gcgtacccga
     1921 ttatccatcg gtggatggag cgactcgtta atcgcttcca tgcgccgcag taacaattgc
     1981 tcaagcagat ttatcgccag cagctccgaa tagcgccctt ccccttgccc ggcgttaatg
     2041 atttgcccaa acaggtcgct gaaatgcggc tggtgcgctt catccgggcg aaagaacccc
     2101 gtattggcaa atattgacgg ccagttaagc cattcatgcc agtaggcgcg cggacgaaag
     2161 taaacccact ggtgatacca ttcgcgagcc tccggatgac gaccgtagtg atgaatctct
     2221 cctggcggga acagcaaaat atcacccggt cggcaaacaa attctcgtcc ctgatttttc
     2281 accaccccct gaccgcgaat ggtgagattg agaatataac ctttcattcc cagcggtcgg
     2341 tcgataaaaa aatcgagata accgttggcc tcaatcggcg ttaaacccgc caccagatgg
     2401 gcattaaacg agtatcccgg cagcagggga tcattttgcg cttcagccat acttttcata
     2461 ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg ccgtcactgc
     2521 gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa aagcattctg
     2581 taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta taatcacggc
     2641 agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta
     2701 tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca
     2761 tacccgtttt tttgggctag cgaattcctg cagcccgggg gatccactag ttctagagcg
     2821 gccgccaccg cggtggagct ccaattcgcc ctatagtgag tcgtattacg cgcgctcact
     2881 ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct
     2941 tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc
     3001 ttcccaacag ttgcgcagcc tgaatggcga atggaaattg taagcgttaa tattttgtta
     3061 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgactgcgat
     3121 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
     3181 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgaaa gcaaattcga
     3241 cccggtcgtc ggttcagggc agggtcgtta aatagccgct tatgtctatt gctggtttac
     3301 cggtttattg actaccggaa gcagtgtgac cgtgtgcttc tcaaatgcct gaggccagtt
     3361 tgctcaggct ctccccgtgg aggtaataat tgacgatatg atcatttatt ctgcctccca
     3421 gagcctgata aaaacggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag
     3481 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg
     3541 aggcggctac agccgatagt ctggaacagc gcacttacgg gttgctgcgc aacccaagtg
     3601 ctaccggcgc ggcagcgtga cccgtgtcgg cggctccaac ggctcgccat cgtccagaaa
     3661 acacggctca tcgggcatcg gcaggcgctg ctgcccgcgc cgttcccatt cctccgtttc
     3721 ggtcaaggct ggcaggtctg gttccatgcc cggaatgccg ggctggctgg gcggctcctc
     3781 gccggggccg gtcggtagtt gctgctcgcc cggatacagg gtcgggatgc ggcgcaggtc
     3841 gccatgcccc aacagcgatt cgtcctggtc gtcgtgatca accaccacgg cggcactgaa
     3901 caccgacagg cgcaactggt cgcggggctg gccccacgcc acgcggtcat tgaccacgta
     3961 ggccgacacg gtgccggggc cgttgagctt cacgacggag atccagcgct cggccaccaa
     4021 gtccttgact gcgtattgga ccgtccgcaa agaacgtccg atgagcttgg aaagtgtctt
     4081 ctggctgacc accacggcgt tctggtggcc catctgcgcc acgaggtgat gcagcagcat
     4141 tgccgccgtg ggtttcctcg caataagccc ggcccacgcc tcatgcgctt tgcgttccgt
     4201 ttgcacccag tgaccgggct tgttcttggc ttgaatgccg atttctctgg actgcgtggc
     4261 catgcttatc tccatgcggt agggtgccgc acggttgcgg caccatgcgc aatcagctgc
     4321 aacttttcgg cagcgcgaca acaattatgc gttgcgtaaa agtggcagtc aattacagat
     4381 tttctttaac ctacgcaatg agctattgcg gggggtgccg caatgagctg ttgcgtaccc
     4441 ccctttttta agttgttgat ttttaagtct ttcgcatttc gccctatatc tagttctttg
     4501 gtgcccaaag aagggcaccc ctgcggggtt cccccacgcc ttcggcgcgg ctccccctcc
     4561 ggcaaaaagt ggcccctccg gggcttgttg atcgactgcg cggccttcgg ccttgcccaa
     4621 ggtggcgctg cccccttgga acccccgcac tcgccgccgt gaggctcggg gggcaggcgg
     4681 gcgggcttcg ccttcgactg cccccactcg cataggcttg ggtcgttcca ggcgcgtcaa
     4741 ggccaagccg ctgcgcggtc gctgcgcgag ccttgacccg ccttccactt ggtgtccaac
     4801 cggcaagcga agcgcgcagg ccgcaggccg gaggcttttc cccagagaaa attaaaaaaa
     4861 ttgatggggc aaggccgcag gccgcgcagt tggagccggt gggtatgtgg tcgaaggctg
     4921 ggtagccggt gggcaatccc tgtggtcaag ctcgtgggca ggcgcagcct gtccatcagc
     4981 ttgtccagca gggttgtcca cgggccgagc gaagcgagcc agccggtggc cgctcgcggc
     5041 catcgtccac atatccacgg gctggcaagg gagcgcagcg accgcgcagg gcgaagcccg
     5101 gagagcaagc ccgtagggcg ccgcagccgc cgtaggcggt cacgactttg cgaagcaaag
     5161 tctagtgagt atactcaagc attgagtggc ccgccggagg caccgccttg cgctgccccc
     5221 gtcgagccgg ttggacacca aaagggaggg gcaggcatgg cggcatacgc gatcatgcga
     5281 tgcaagaagc tggcgaaaat gggcaacgtg gcggccagtc tcaagcacgc ctaccgcgag
     5341 cgcgagacgc ccaacgctga cgccagcagg acgccagaga acgagcactg ggcggccagc
     5401 agcaccgatg aagcgatggg ccgactgcgc gagttgctgc cagagaagcg gcgcaaggac
     5461 gctgtgttgg cggtcgagta cgtcatgacg gccagcccgg aatggtggaa gtcggccagc
     5521 caagaacagc aggcggcgtt cttcgagaag gcgcacaagt ggctggcgga caagtacggg
     5581 gcggatcgca tcgtgacggc cagcatccac cgtgacgaaa ccagcccgca catgaccgcg
     5641 ttcgtggtgc cgctgacgca ggacggcagg ctgtcggcca aggagttcat cggcaacaaa
     5701 gcgcagatga cccgcgacca gaccacgttt gcggccgctg tggccgatct agggctgcaa
     5761 cggggcatcg agggcagcaa ggcacgtcac acgcgcattc aggcgttcta cgaggccctg
     5821 gagcggccac cagtgggcca cgtcaccatc agcccgcaag cggtcgagcc acgcgcctat
     5881 gcaccgcagg gattggccga aaagctggga atctcaaagc gcgttgagac gccggaagcc
     5941 gtggccgacc ggctgacaaa agcggttcgg caggggtatg agcctgccct acaggccgcc
     6001 gcaggagcgc gtgagatgcg caagaaggcc gatcaagccc aagagacggc ccgag
//
