LOCUS       V005347                 9512 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005347
VERSION     V005347
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9512)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Multichromatic Bacterial Photography
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9512)
  AUTHORS   Fernandez-Rodriguez J, Moser F, Voigt CA.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-MAR-2016) Biological Engineering, MIT, 500 Tech
            Square, Rm 140, Cambridge, MA 02139, USA
REFERENCE   3  (bases 1 to 9512)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9512)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (31-MAR-2016) Biological Engineering, MIT, 500 Tech Square, Rm 140,
            Cambridge, MA 02139, USA"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9512
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    231..278
                     /label="Terminator L3S1P22"
                     /note="Terminator L3S1P22"
     CDS             complement(285..1031)
                     /codon_start=1
                     /product="pcyA"
                     /label="pcyA"
                     /protein_id="ARG47676.1"
                     /translation="MAVTDLSLTNSSLMPTLNPMIQQLALAIAASWQSLPLKPYQLPED
                     LGYVEGRLEGEKLVIENRCYQTPQFRKMHLELAKVGKWLDILHCVMFPEPLYGLPLFGC
                     DIVAGPGGVSAAIADLSPTQSDRQLPAAYQKSLAELGQPEFEQQRELPPWGEIFSEYCL
                     FIRPSNVTEEERFVQRVVDFLQIHCHQSIVAEPLSEAQTLEHRQGQIHYCQQQQKNDKT
                     RRVLEKAFGEAWAERYMSQVLFDVIQ"
     RBS             1038..1049
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             complement(1055..1774)
                     /gene="pbsA1"
                     /label="Heme oxygenase 1"
                     /note="Heme oxygenase 1 from Synechocystis sp. (strain PCC
                     6803 / Kazusa). Accession#: P72849"
     RBS             1781..1792
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     regulatory      complement(1809..1843)
                     /label="Promoter J23108"
                     /note="Promoter J23108"
                     /regulatory_class="promoter"
     promoter        complement(1809..1842)
                     /label="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     regulatory      1894..3050
                     /label="Promoter PompC1157"
                     /note="Promoter PompC1157"
                     /regulatory_class="promoter"
     CDS             3083..3793
                     /label="lambda repressor"
                     /note="phage lambda repressor"
     CDS             3794..3826
                     /label="ssrA tag (LVA)"
                     /note="C-terminal peptide that mediates degradation in
                     bacteria through the ClpXP and ClpAP proteases (McGinness
                     et al., 2006)"
     misc_feature    3863..4026
                     /label="Terminator DT11"
                     /note="Terminator DT11"
     misc_feature    4065..4086
                     /label="BioBrick prefix"
                     /note="BioBrick prefix for parts that do not start with
                     'ATG'"
     regulatory      4087..4168
                     /label="Promoter Bba_J64067"
                     /note="Promoter Bba_J64067"
                     /regulatory_class="promoter"
     misc_feature    4208..4333
                     /label="similar to SynZip 18"
                     /note="similar to SynZip 18"
     misc_feature    4334..4348
                     /label="similar to GGSGG linker"
                     /note="similar to GGSGG linker"
     misc_feature    4349..5203
                     /label="similar to K1F Fragment of T7 RNAP"
                     /note="similar to K1F Fragment of T7 RNAP"
     misc_feature    5213..5259
                     /label="Terminator L3S3P11"
                     /note="Terminator L3S3P11"
     terminator      5378..5407
                     /label="T3Te terminator"
                     /note="phage T3 early transcription terminator"
     terminator      complement(5467..5526)
                     /label="his operon terminator"
                     /note="This putative transcriptin terminator from the E.
                     coli his operon has a 2-bp deletion introduced during
                     synthesis. Its efficiency has not been determined."
     misc_feature    5548..5578
                     /label="Terminator tR2"
                     /note="Terminator tR2"
     CDS             complement(5595..5828)
                     /label="TpR"
                     /note="E. coli plasmid-associated dihydrofolate reductase"
     promoter        complement(5856..5890)
                     /label="J23119 promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119)"
     misc_feature    complement(6024..6548)
                     /label="nuc1"
                     /note="nuc1"
     misc_feature    complement(6545..7174)
                     /label="kfrA"
                     /note="kfrA"
     rep_origin      complement(7420..7855)
                     /direction=LEFT
                     /label="pSa ori"
                     /note="origin of replication from bacterial plasmid pSa"
     CDS             complement(7895..8863)
                     /label="RepA"
                     /note="replication protein of plasmid pSa"
     misc_feature    complement(8856..9512)
                     /label="resP"
                     /note="resP"
ORIGIN
        1 ttgcagggaa aacgtcagat tcggttaaac atgcctcatt ctagcgcaga ttaaatagga
       61 attaaatacc ctgtagcggt atagataaaa cgttggtttg gagctctggt ctgacagctc
      121 tagcagaaat catccttagc gaaagctaag gatttttttt atctgattac cgcctttgag
      181 tgagcaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc ttttgttatc
      241 aataaaaaag gccgcgattt gcgtccttat tgttcgtctc tagattattg gataacatca
      301 aataagactt ggctcatata ccgttccgcc caagcttccc caaaagcttt ttccagtacc
      361 cgacgggttt tatcattttt ctgttgttgt tggcagtaat gaatttgccc ctgacggtgc
      421 tccaaagttt gagcttcaga caagggttcg gcaacgatgg attggtgaca atgaatttgc
      481 aaaaagtcca ctaccctttg tacaaatctt tcttcttcag tgacattgct gggacggatg
      541 aataaacaat attcagaaaa tatttctccc caggggggca attcccgttg ttgctcaaat
      601 tctggctggc ctagctctgc caatgatttt tggtacgctg cgggcaattg gcgatcgctt
      661 tgggtggggg atagatccgc aatagccgca cttactccac cggggccggc cacaatgtca
      721 cagccaaaca aaggtagacc gtataaaggc tcaggaaaca ttacacagtg gagaatatcc
      781 aaccatttgc ccaccttggc caactccaaa tgcattttgc gaaactgggg cgtttggtag
      841 caccgatttt caatcactaa cttttcccct tccaggcggc cttctacgta gcccaaatcc
      901 tccggcaatt gatagggctt gaggggtaaa ctttgccaac tagcggcgat cgccagggcc
      961 aactgttgaa tcatcgggtt caacgtgggc atcagggaag aattggtcaa acttaaatca
     1021 gtgacggcca tggatccttt ctcctcttta actagccttc ggaggtggcg aggccaactt
     1081 cggtgctgcc ttgactgcgg cgacgggtga ggctgttgaa caccataatg ccgatcgcct
     1141 tgatcaggtt gccttcaagt tcgttgaaca ttttcatgtt catggcaaag gcgtcattgg
     1201 cttcatccac aatccgttcg gcggtggctt ggttaatggg cagatcattc atagcttgac
     1261 ggtaggtatt tttaaaagcc ttttcgtcat caatgtcggc aaattcatag aaagctgtgc
     1321 caccatcgtg gagattcatg gcattttggg caattttctt gagaatttga ccgccggaaa
     1381 gatcccccag gtaacgggtg taggaatggg ccaccaacaa ttcaggggcc gtagcggcca
     1441 cttgccggac tcggtccaca taggcttggc cagcggcaga aattttcact tcttgccgcc
     1501 agttggagcc gtaatagaat tgcaggtctt gctctaggct ttgtttgcgg ttgagttcgg
     1561 ggaagtaaat gtggctgagg atgggatggt ccttaaattt tgccatttcc tcttccatgg
     1621 cactgtagac aaagtagaga ttgccaacca gcttacggta ggaatttttc tcgacaacgc
     1681 ccttgaggaa gcatttgaca aagccgacgt tctccgccat ggagtgggat tttttcgtcc
     1741 cttcccgcaa ctgggaagct aagttgacac tcatggtacc tttctcctct ttaatgaatt
     1801 ctgtgtgagc tagcattata cctaggactg agctagctgt cagttttttt gacggtaaag
     1861 ccaagctgaa ggctgacaaa atctatactg ttatcccttg catttacatt ttgaaacatc
     1921 tatagcgata aatgaaacat cttaaaagtt ttagtatcat attcgtgttg gattattctg
     1981 catttttggg gagaatggac ttgccgactg attaatgagg gttaatcagt atgcagtggc
     2041 ataaaaaagc aaataaaggc atataacaga gggttaataa catgaaagtt aaagtactgt
     2101 ccctcctggt cccagctctg ctggtagcag gcgcagcaaa cgctgctgaa gtttacaaca
     2161 aagacggcaa caaattagat ctgtacggta aagtagacgg cctgcgctat ttctctgaca
     2221 acaaagatgt agatggcgac cagacctaca tgcgtcttgg cttcaaaggt gaaactcagg
     2281 ttactgacca gctgaccggt tacggccagt gggaatatca gatccagggc aacagcgctg
     2341 aaaacgaaaa caactcctgg acccgtgtgg cattcgcagg tctgaaattc caggatgtgg
     2401 gttctttcga ctacggtcgt aactacggcg ttgtttatga cgtaacttcc tggaccgacg
     2461 tactgccaga attcggtggt gacacctacg gttctgacaa cttcatgcag cagcgtggta
     2521 acggcttcgc gacctaccgt aacactgact tcttcggtct ggttgacggc ctgaactttg
     2581 ctgttcagta ccagggtaaa aacggcaacc catctggtga aggctttact agtggcgtaa
     2641 ctaacaacgg tcgtgacgca ctgcgtcaaa acggcgacgg cgtcggcggt tctatcactt
     2701 atgattacga aggtttcggt atcggtggtg cgatctccag ctccaaacgt actgatgctc
     2761 agaacaccgc tgcttacatc ggtaacggcg accgtgctga aacctacact ggtggtctga
     2821 aatacgacgc taacaacatc tacctggctg ctcagtacac ccagacctac tgaagcggcg
     2881 cacgaaaaac gcgaaagcta aagttttcgc atttatcgtg aaacgctttc gcgtttttcg
     2941 tgcgccgctt caccggataa agagcagcta ctggtggtta acctttccgg tcgcggcgat
     3001 aaagacatct tcaccgttca cgatattttg aaagcacgag gggaaatctg ccagcctaca
     3061 aacgagagag gagggttacg ctatgagcac aaaaaagaaa ccattaacac aagagcagct
     3121 tgaggacgca cgtcgcctta aagcaattta tgaaaaaaag aaaaatgaac ttggcttatc
     3181 ccaggaatct gtcgcagaca agatggggat ggggcagtca ggcgttggtg ctttatttaa
     3241 tggcatcaat gcattaaatg cttataacgc cgcattgctt gcaaaaattc tcaaagttag
     3301 cgttgaagaa tttagccctt caatcgccag agaaatctac gagatgtatg aagcggttag
     3361 tatgcagccg tcacttagaa gtgagtatga gtaccctgtt ttttctcatg ttcaggcagg
     3421 gatgttctca cctgagctta gaacctttac caaaggtgat gcggagagat gggtaagcac
     3481 aaccaaaaaa gccagtgatt ctgcattctg gcttgaggtt gaaggtaatt ccatgaccgc
     3541 accaacaggc tccaagccaa gctttcctga cggaatgtta attctcgttg accctgagca
     3601 ggctgttgag ccaggtgatt tctgcatagc cagacttggg ggtgatgagt ttaccttcaa
     3661 gaaactgatc agggatagcg gtcaggtgtt tttacaacca ctaaacccac agtacccaat
     3721 gatcccatgc aatgagagtt gttccgttgt ggggaaagtt atcgctagtc agtggcctga
     3781 agagacgttt ggcgctgcaa acgacgaaaa ctacgcttta gtagcttaat aacggcacct
     3841 cgctaacgga ttcaccgtta ctaacgcatg agaaagcccc cggaagatca ccttccgggg
     3901 gcttttttat tgcgctcctt ggccctccat ccttagatag ctcggtacca aattccagaa
     3961 aagaggcctc ccgaaagggg ggcctttttt cgttttggtc ctcataggca atacgatcgc
     4021 atgtccaatg catggctgtt gtttgtcggt gaacctctcc tgaggaattc gcggccgctt
     4081 ctagagtaag cacctgtagg atcgtacagg ttgacggcaa gaaaatataa caccgtgcgt
     4141 gttgactatt ttacctctgg cggtgatact acaaataatt ttgtttaaga aagaggagga
     4201 ataatctatg agcatcgcgg cgaccctgga gaacgacctg gcgcgcctgg aaaacgagaa
     4261 tgcgcgtctg gaaaaagata tcgcaaacct ggaacgtgac ctcgcgaaac tggaacgcga
     4321 agaagcttac ttcggtggtt ctggtggtaa aaacactggt gagatctcag aaaaagttaa
     4381 actgggcacc aaagcgcttg ctggtcagtg gttggcgtat ggtgttaccc gttccgtgac
     4441 aaaacgttct gtgatgaccc tggcatacgg ctcaaaagaa ttcggctttc gtcagcaggt
     4501 acttgaagac accatccagc cggccatcga ctctggcaaa ggtctgatgt tcactcagcc
     4561 gaaccaggcg gctggttaca tggccaaact gatctgggaa tccgtttctg ttactgtcgt
     4621 agcggcggtc gaggctatga actggctgaa gtcggccgcc aaactgctgg ccgcagaagt
     4681 gaaagataaa aaaaccgggg aaatccttcg taaacgctgt gcggtgcact gggttacccc
     4741 ggatggtttt ccggtatggc aggagtacaa aaagccgatt cagactcgcc tgaacctgcg
     4801 cttcctgggc tctttcaacc tgcagccgac ggtaaacacg aacaaagaca gcgaaatcga
     4861 cgcgcacaaa caagaaagcg gcattgctcc aaacttcgtg cactctcagg atggctccca
     4921 tctgcgtaaa accgtagtct gggcacatga gaaatacggt attgaaagct ttgcgttaat
     4981 tcatgatagc tttggcacta tcccagcaga tgccgcgaac ctgttcaaag ctgtccgtga
     5041 aaccatggtt gacacttatg aatcttgtga cgttctagca gatttttacg atcagttcgc
     5101 ggatcagctg catgaatctc aactggacaa aatgccggcc ctgccggcca aaggtaacct
     5161 gaacctgcgt gacatcctgg agtctgactt cgcgttcgca taataatcta gaccaattat
     5221 tgaacaccct tcggggtgtt tttttgtttc tggtctcccc ctaggcgttc tctagataaa
     5281 agccgaattc cagcacactg gcggccgtta taacaaacta gcaacaccag agagtcacta
     5341 agggctaact aactaattac gtagcaatca actcactggc tcaccttcac gggtgggcct
     5401 ttcttcggca cgggcaaatt gctgaatatt ccttttctta gacgtcaggt ggcaaacgcg
     5461 aagtaatctt ttcggtttta aagaaaaagg gcagggtggt gacaccttgc ccgttttttt
     5521 gccggagggt accatttgtg tagggctaaa aaaaaaggcc tgcgattacc agcaggcctg
     5581 ttgagagatc cttaggccac acgttcaagt gcagccacag gataaatttg cactgagcct
     5641 gggtgggatt cggactcgac cgcatagcct tcaggagtga gttttgtgca ataccaaccg
     5701 acgacttgac cctgccaagc ggcaccagat ttcttgcgta cgcgatcccc taagccaaag
     5761 gtggcactca ggggaagcgc aaactgccct gcaacgggag cgttggcttc atcgctactt
     5821 tgacccatac ttttccttct ccttattaaa gatccgctag cattatacct aggactgagc
     5881 tagctgtcaa aaaaccgcca ctgcgccgtt accaccgctc gaggcgctgt gcgctatggc
     5941 gatctggaag tgtcgggacg aggggcgtcc cggcggtaaa ttcgacgtgc ctgcggcgcg
     6001 tcgaacaagg ggcgtgcccg gtgtcagttc gtggcatttt tcgaggcgcg acgccatttc
     6061 caaggctcct gagcattcgg gtctgaccaa aggccgagcc gttggcggcg ggcctctttt
     6121 tcatactcgt acatctgcgc gtcggttggt ttgccgtaat aacggtaagc ccaggccatg
     6181 ccttcttgaa ccatgatcgc attgatgttg gtgagttgtg tttggccgcc ggggtattgc
     6241 aacggcgcgt aaacgacccc aagagtgcgg ccataccgat caacctcttt ttcggtcact
     6301 tgaacctctt ggcgaaaggt caagtcggcg agccgttggc gagcacggga gccgaaggct
     6361 tggccgcttt ccggtgcgtc aatatcggcc aatctcacgc ggatggtctg acggttcacc
     6421 aaaacgtcga tagtgtcacc gtcaaggatt cggacgactt caccccggaa gtcggcccaa
     6481 gcgggcacac tgacgattag gacgacagcg gccgcgaccg cgcgaagggc ggcaagggcg
     6541 cttttcattg tttgcctcct gttttcaaga cggctgtgag attggcgacc tgctctttga
     6601 gggcttccac ctgaccttgc agactggcgg cgcgctcgat ggcctccttg gcctgtttgc
     6661 gggcctcgat agcctcgtta tcgcgttggg tgagcttttc catgcagcgg tttagttctt
     6721 cgccgctgcg gcgtttcact tcggcaagct ggtcggccag cttgtcgcgc tcgcgttcca
     6781 taggttcgag ctgattcact cgttcgcgga gctggtcgtt ttcgcgggtg aaggtgtcgg
     6841 ctagttcgat tgcttcggca agctgctggc tgatggccgc tttgtcggcc tcgatctgtt
     6901 tccgatcttc gtcaaaccgg gcgttggcgt gcgccagggc gatagcccat agcgcattgc
     6961 caagctcggc aagatgctcg ttgactgcaa ccggcaatgg gtctgatgag ggcagggtgg
     7021 cggtcttgcg gtttttccat tcagccattg catcggaaat ggttgtgaag ctaccgcttc
     7081 cgagtttctt gcgcacggcg gccaaagtgg gccggatgcc ttcggcgtcc agttcgtcgg
     7141 ctgctcgcca aatgtcttgt ttagtgattg ccattcttgc gggcctctgt actgtagtat
     7201 gttgtatgat actacatact acaacaattt aacagagcca tcttggaatc tggtgtctct
     7261 gcgcctataa ttctggaaca gctactttcc gaacgactcc tgcgttgatc ggaaatccag
     7321 aagcccgaga ggttgccgcc tttcgggctt tttctttttc aaaaaaaaaa atttataaaa
     7381 cgatctgttg cggccgccgg gttgtgggca aaggcgctgg cgctcgacgg tgggcaaccg
     7441 cttgcggttg tccacgggcg gagccggtgc gcgtagcgca ttgtccacaa gccaagggcg
     7501 accaataatt gatatatata ttcataattg aaaagctaat tgaacatact acttgctgta
     7561 actacttgcc ggagcgaggg gtgtttgcaa gctgttgatc tgaaagggct attagcgttc
     7621 tcacgtgcct ttttgattag cgatttcacg tgaccttatt agcgatttca cgtactccga
     7681 ttagcgattt cacgtaccct gattagcgat ttcacgtgga tagtttttgg agcgggccgg
     7741 aaagccccgt gaatcaaggc tttgcggggc attagcggtt tcacgtggat aactaccctc
     7801 tatccacagg cttccgggga taaaaaagcc cgctcgacgg cgggctgttg gatgggaagg
     7861 cttgaccaag ccaagcgtag cgttggcctg gtcaagtcgg aggggggccg atgcgagcgc
     7921 ccttgccggg tgcgcgggtg acatgcaggc gtgtggattt gatgcgcagg cattcgccgt
     7981 catcttcgat gcagtcgctt gcctcgggat agacaatcaa cacttcgcgt aggcgctttt
     8041 tgaagttgta tttgaagctg gcgagtgctg cccgctctgc ccgctctcgg gccttatcgt
     8101 ccagttcggg cgagttgcgt gcgcggctgc cataggatga gccgaattgc gcttgcaggg
     8161 cgacccaagg gatttgcacg aaggggcggc ccttggcccg caacaggaac acgcgatagg
     8221 tcagccacgt gtaaatgtcc atcgcaagcg gagactgccg caaggcatgc aggtagtcga
     8281 ttcggatagg aaccggtgag cgggtgactt cctcgaagaa atcgcctgtg agggtgaggg
     8341 tgctatccca tagcgcccga tcttctggcc gcttgggatt ccagaataga aaagcgcgct
     8401 tggcaatgac gacgttctca atgccgaagt cattgccttg ctcgccggca agcgaaatca
     8461 tggatgaaaa caggcgttgc gcctgattgc gaagggtggc cgtgtaacgg ccatcggtgt
     8521 gcattccgag cctttgtaga aattccgatt gcgaccggcc aaggttcaac acggggtctt
     8581 tcgttcgcac ggcctcggtg catatccaag caagcaaggt gcgcggcata gaaccgtagg
     8641 gcaggccgat gctcggcttg cccatgatcg acaaggtgac gatgccattg gtgcgctcaa
     8701 agtagctggt cttggggtcg gtgtggggca tggtcgcttg cacaaggcaa cgggccatgt
     8761 agccgactaa gccagcttcg cgggcatcct ccatttcgag cgcgaggctc gtcttgatga
     8821 tctcgttgat acgatggccg ggggctttgt tgttcttagg catgttgttc cctccccggc
     8881 atggtgatgg ttggtctagt gtttgtgggt ttgatgttcc ggcgtttgat gaacaggcgc
     8941 aaggtgtgag ggctgacgcc taacaactcg gctgcgcgac tttgcggcaa gccaaggttc
     9001 acgtatgcct gtacttcatc aatacggctg tccagcttca aggcgctcga tttgctgccc
     9061 ttgggtcgcc cgagcgtctt gccgcgctct ctggcgactt gtagcgcctc ggtggtacgt
     9121 gcctgaatga aatgccgctc gatctgtgca gccaagccaa gcacggttgc catgatgtcg
     9181 ctttgtaggc tgccgtccat gatgatcttc tgtttggtca catggacgat taggccgcgc
     9241 tcgctcgccg ctttgagaat ttccaaggcg gcgagggcgg aaccggcaat gcgcgtaatc
     9301 tccggcgtca gtagcacgtc gccacgctcg gccttttcga tgattgctcc gagcttgcgc
     9361 ttgcgccagt cctttgctct gctggcaatt tcttcctcga tctgtagcgg cgcgaagcct
     9421 ttggcgttcg cgtattcgag caaaccgtat ttttggtttt ccgggtcttg gccgtcacgc
     9481 gaaacccgga gataggcata gtattttggc at
//
