LOCUS       pJET1.2.        2974 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Positive selection cloning vector with a lethal insert that allows 
            for efficient recovery of blunt-ended PCR products.
ACCESSION   .
VERSION     .
KEYWORDS    pJET1.2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Thermo Scientific (Fermentas)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Linearize at Eco32I (EcoRV) to insert a blunt PCR product.
FEATURES             Location/Qualifiers
     source          1..2974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        305..323
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    328..422
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site with a central Eco32I (EcoRV)
                     site"
     protein_bind    complement(782..798)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(806..836)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    complement(851..872)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1163..1751)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1925..2782)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2783..2887)
                     /label=AmpR promoter
ORIGIN
        1 gcccctgcag ccgaattata ttatttttgc caaataattt ttaacaaaag ctctgaagtc
       61 ttcttcattt aaattcttag atgatacttc atctggaaaa ttgtcccaat tagtagcatc
      121 acgctgtgag taagttctaa accatttttt tattgttgta ttatctctaa tcttactact
      181 cgatgagttt tcggtattat ctctattttt aacttggagc aggttccatt cattgttttt
      241 ttcatcatag tgaataaaat caactgcttt aacacttgtg cctgaacacc atatccatcc
      301 ggcgtaatac gactcactat agggagagcg gccgccagat cttccggatg gctcgagttt
      361 ttcagcaaga tatctttcta gaagatctcc tacaatattc tcagctgcca tggaaaatcg
      421 atgttcttct tttattctct caagattttc aggctgtata ttaaaactta tattaagaac
      481 tatgctaacc acctcatcag gaaccgttgt aggtggcgtg ggttttcttg gcaatcgact
      541 ctcatgaaaa ctacgagcta aatattcaat atgttcctct tgaccaactt tattctgcat
      601 tttttttgaa cgaggtttag agcaagcttc aggaaactga gacaggaatt ttattaaaaa
      661 tttaaatttt gaagaaagtt cagggttaat agcatccatt ttttgctttg caagttcctc
      721 agcattctta acaaaagacg tctcttttga catgtttaaa gtttaaacct cctgtgtgaa
      781 attgttatcc gctcacaatt ccacacatta tacgagccgg aagcataaag tgtaaagcct
      841 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg ccaattgctt
      901 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
      961 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1021 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1081 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1141 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1201 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1261 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1321 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1381 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1441 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1501 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1561 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     1621 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1681 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1741 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     1801 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     1861 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     1921 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     1981 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2041 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2101 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2161 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2221 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2281 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2341 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2401 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2461 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2521 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2581 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2641 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2701 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     2761 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     2821 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     2881 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
     2941 cattaaccta taaaaatagg cgtatcacga ggcc
//
