LOCUS       .                       3983 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..176
                     /label="J23119(SpeI) promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     184..200
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1323..1340
                     /label="6xHis"
                     /note="6xHis affinity tag"
     primer_bind     complement(1350..1366)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      1617..1703
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1795..1822
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2077..2181
                     /label="AmpR promoter"
     CDS             2182..3039
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3213..3801
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg cttgacagct agctcagtcc taggtataat actagttttc
      181 acacaggaaa cagctatgac catgattacg ccaagcttat gagaaaacta ctaattttta
      241 gtatatctgc ttacttgatg gcaggtatcg tttcgtgtaa aggtgtagac agtgcaacac
      301 ctgtaacaga agatcgctta gctctgaatg cggtaaatgc tccggcagat aataccgtaa
      361 atattaaaac attcgacaaa gtaaaaaatg cctttggtga cggattgtcc caaagtgcag
      421 aaggaacctt tacatttccg gccgatgtaa caactgtaaa aacgattaag atgttcatca
      481 aaaatgaatg tcctaataaa acctgtgatg aatgggatcg ttatgccaat gtttatgtaa
      541 aaaataaaac aacaggagaa tggtatgaaa taggacgctt tattactcca tattgggtgg
      601 gtacggaaaa attacctcgt ggactggaaa ttgatgttac cgatttcaaa tctttactgt
      661 cgggaaatac agaacttaaa atttatacgg agacttggtt ggccaaagga agagaataca
      721 gtgtagactt tgatattgta tatggtacac cggattataa atattcggca gtagtacctg
      781 taatccaata taacaaatca tccattgatg gtgttcctta tggtaaagca catacactgg
      841 gattaaaaaa gaatattcag ttaccaacaa acacggaaaa agcttatctt agaactacta
      901 tttccggatg gggacatgcc aagccatatg atgcgggaag caggggctgt gcagaatggt
      961 gcttcagaac acatactata gcaataaata atgcgaatac tttccaacac cagctgggtg
     1021 ctttaggatg ttcagcaaac cctattaata atcagagtcc gggaaattgg gctcctgaca
     1081 gagcagggtg gtgtccggga atggcagtgc caacacgtat agatgtgttg aataactctt
     1141 taacgggtag tacttttagt tatgaatata agttccagag ttggacaaac aacggaacca
     1201 atggagatgc tttttatgca atttccagtt ttgtgattgc aaaaagtaat acacctatta
     1261 gtgctccggt agttacaaac gatcacctgg cgtctctgtg gtggggtact gaactgctcg
     1321 agcaccacca ccaccaccac tgagaattca ctggccgtcg ttttacaacg tcgtgactgg
     1381 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     1441 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     1501 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     1561 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taatttttcc aggcatcaaa
     1621 taaaacgaaa gtctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1681 acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg aagcaacggc
     1741 ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt aagcagaagg
     1801 ccatcctgac ggatggcctt tttgcgtttc tacaaactct gccagccccg acacccgcca
     1861 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct
     1921 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg
     1981 agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt
     2041 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2101 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     2161 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2221 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2281 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2341 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2401 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2461 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2521 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2581 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2641 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2701 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     2761 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     2821 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     2881 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     2941 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3001 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3061 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3121 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3181 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3241 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3301 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3361 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3421 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3481 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3541 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3601 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3661 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3721 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     3781 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     3841 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     3901 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     3961 tcagtgagcg aggaagcgga aga
//
