LOCUS       .                       4277 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..176
                     /label="J23119(SpeI) promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     184..200
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1620..1637
                     /label="6xHis"
                     /note="6xHis affinity tag"
     primer_bind     complement(1644..1660)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      1911..1997
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2089..2116
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2371..2475
                     /label="AmpR promoter"
     CDS             2476..3333
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3507..4095
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg cttgacagct agctcagtcc taggtataat actagttttc
      181 acacaggaaa cagctatgac catgattacg ccaagcttat gactgaaatc gttgcagata
      241 aaacggtaga agtagttaaa aacgcaatcg aaaccgcaga tggagcatta gatctttata
      301 ataaatatct cgatcaggtc atcccctggc agacctttga tgaaaccata aaagagttaa
      361 gtcgctttaa acaggagtat tcacaggcag cctccgtttt agtcggcgat attaaaacct
      421 tacttatgga tagccaggat aagtattttg aagcaaccca aacagtgtat gaatggtgtg
      481 gtgttgcgac gcaattgctc gcagcgtata ttttgctatt tgatgagtac aatgagaaga
      541 aagcatccgc ccagaaagac attctcatta aggtactgga tgacggcatc acgaagctga
      601 atgaagcgca aaaatccctg ctggtaagct cacaaagttt caacaacgct tccgggaaac
      661 tgctggcgtt agatagccag ttaaccaatg atttttcaga aaaaagcagc tatttccagt
      721 cacaggtaga taaaatcagg aaggaagcat atgccggtgc cgcagccggt gtcgtcgccg
      781 gtccatttgg attaatcatt tcctattcta ttgctgcggg cgtagttgaa ggaaaactga
      841 ttccagaatt gaagaacaag ttaaaatctg tgcagaattt ctttaccacc ctgtctaaca
      901 cggttaaaca agcgaataaa gatatcgatg ccgccaaatt gaaattaacc accgaaatag
      961 ccgccatcgg tgagataaaa acggaaactg aaacaaccag attctacgtt gattatgatg
     1021 atttaatgct ttctttgcta aaagaagcgg ccaaaaaaat gattaacacc tgtaatgagt
     1081 atcagaaaag acacggtaaa aagacactct ttgaggtacc tgaagtcgga tccgaattca
     1141 gcagcagcag cggtagcagc agcagcggca tgtccctcac cttctaccca gcctggctca
     1201 cagtgtcaga gggagcaaat gccaccttca cctgcagctt gtccaactgg tcggaggatc
     1261 ttatgctgaa ctggaaccgc ctgagtccca gcaaccagac tgaaaaacag gccgccttct
     1321 gtaatggttt gagccaaccc gtccaggatg cccgcttcca gatcatacag ctgcccaaca
     1381 ggcatgactt ccacatgaac atccttgaca cacggcgcaa tgacagtggc atctacctct
     1441 gtggggccat ctccctgcac cccaaggcaa aaatcgagga gagccctgga gcagagctcg
     1501 tggtaacaga gagaatcctg gagacctcaa caagatatcc cagcccctcg cccaaaccag
     1561 aaggccggtt tcaaggcatg gtcggatccg aattcagcag cggtagcagc agcagcggcc
     1621 accaccacca ccaccacgaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     1681 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     1741 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     1801 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatggtgc
     1861 actctcagta caatctgctc tgatgccgca tagttaattt ttccaggcat caaataaaac
     1921 gaaagtctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc
     1981 tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag
     2041 ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag aaggccatcc
     2101 tgacggatgg cctttttgcg tttctacaaa ctctgccagc cccgacaccc gccaacaccc
     2161 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     2221 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     2281 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     2341 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     2401 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     2461 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     2521 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     2581 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     2641 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     2701 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     2761 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     2821 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     2881 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     2941 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     3001 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     3061 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     3121 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     3181 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     3241 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     3301 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     3361 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     3421 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     3481 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     3541 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     3601 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     3661 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     3721 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     3781 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     3841 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     3901 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     3961 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     4021 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     4081 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     4141 ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc
     4201 gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg
     4261 agcgaggaag cggaaga
//
