LOCUS       .                       4934 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..176
                     /label="J23119(SpeI) promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     184..200
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             1170..2291
                     /gene="L-lactate oxidase"
                     /label="L-lactate oxidase"
                     /note="L-lactate oxidase from Aerococcus viridans (strain
                     ATCC 11563 / DSM 20340 / CCUG 4311 / JCM 20461 / NBRC 12219
                     / NCTC 8251 / M1). Accession#: Q44467"
     primer_bind     complement(2301..2317)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      2568..2654
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2746..2773
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        3028..3132
                     /label="AmpR promoter"
     CDS             3133..3990
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      4164..4752
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg cttgacagct agctcagtcc taggtataat actagttttc
      181 acacaggaaa cagctatgac catgattacg ccaagcttat gactgaaatc gttgcagata
      241 aaacggtaga agtagttaaa aacgcaatcg aaaccgcaga tggagcatta gatctttata
      301 ataaatatct cgatcaggtc atcccctggc agacctttga tgaaaccata aaagagttaa
      361 gtcgctttaa acaggagtat tcacaggcag cctccgtttt agtcggcgat attaaaacct
      421 tacttatgga tagccaggat aagtattttg aagcaaccca aacagtgtat gaatggtgtg
      481 gtgttgcgac gcaattgctc gcagcgtata ttttgctatt tgatgagtac aatgagaaga
      541 aagcatccgc ccagaaagac attctcatta aggtactgga tgacggcatc acgaagctga
      601 atgaagcgca aaaatccctg ctggtaagct cacaaagttt caacaacgct tccgggaaac
      661 tgctggcgtt agatagccag ttaaccaatg atttttcaga aaaaagcagc tatttccagt
      721 cacaggtaga taaaatcagg aaggaagcat atgccggtgc cgcagccggt gtcgtcgccg
      781 gtccatttgg attaatcatt tcctattcta ttgctgcggg cgtagttgaa ggaaaactga
      841 ttccagaatt gaagaacaag ttaaaatctg tgcagaattt ctttaccacc ctgtctaaca
      901 cggttaaaca agcgaataaa gatatcgatg ccgccaaatt gaaattaacc accgaaatag
      961 ccgccatcgg tgagataaaa acggaaactg aaacaaccag attctacgtt gattatgatg
     1021 atttaatgct ttctttgcta aaagaagcgg ccaaaaaaat gattaacacc tgtaatgagt
     1081 atcagaaaag acacggtaaa aagacactct ttgaggtacc tgaagtcgga tccgaattca
     1141 gcagcagcag cggtagcagc agcagcggca tgaataacaa tgacattgaa tataatgcac
     1201 ctagtgaaat caagtacatt gatgttgtca atacttacga cttagaagaa gaagcaagta
     1261 aagtggtacc acatggtggt tttaactata ttgccggtgc atctggtgat gagtggacta
     1321 aacgcgctaa tgaccgtgct tggaaacata aattactata cccacgtcta gcgcaagatg
     1381 ttgaagcgcc cgatacaagt actgaaattt taggtcataa aattaaagcc ccattcatca
     1441 tggcaccaat tgctgcacat ggtttagccc acactactaa agaagctggt actgcacgtg
     1501 cagtttcaga atttggtaca attatgtcca tctcagctta ttctggtgca acatttgaag
     1561 aaatttctga aggcttaaat ggcggacccc gttggttcca aatctatatg gctaaagatg
     1621 accaacaaaa ccgtgatatc ttagacgaag ctaaatctga tggtgcaact gctatcatcc
     1681 ttacagctga ctcaactgtt tctggaaacc gtgaccgtga tgtgaagaat aaattcgttt
     1741 acccatttgg tatgccaatt gttcaacgtt acttacgtgg tacagcagaa ggtatgtcat
     1801 taaacaatat ctacggtgct tcaaaacaaa aaatctcacc aagagatatt gaggaaatcg
     1861 ccgctcattc tggattacca gtattcgtta aaggtattca acacccagaa gatgcagata
     1921 tggcaatcaa agctggtgca tcaggtatct gggtatctaa ccacggtgct cgtcaactat
     1981 atgaagctcc aggttcattt gacacccttc cagctattgc tgaacgtgta aacaaacgtg
     2041 taccaatcgt ctttgattca ggtgtacgtc gtggtgaaca cgttgccaaa gcgctagctt
     2101 caggggcaga cgttgttgct ttaggacgcc cagtcttatt tggtttagct ttaggtggct
     2161 ggcaaggtgc ttactcagta cttgactact tccaaaaaga cttaacacgc gtaatgcaat
     2221 taacaggttc acaaaatgtg gaagacttga agggtctaga tttattcgat aacccatacg
     2281 gttatgaata ctaggaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     2341 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     2401 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     2461 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact
     2521 ctcagtacaa tctgctctga tgccgcatag ttaatttttc caggcatcaa ataaaacgaa
     2581 agtctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc
     2641 tgagtaggac aaatccgccg ggagcggatt tgaacgttgc gaagcaacgg cccggagggt
     2701 ggcgggcagg acgcccgcca taaactgcca ggcatcaaat taagcagaag gccatcctga
     2761 cggatggcct ttttgcgttt ctacaaactc tgccagcccc gacacccgcc aacacccgct
     2821 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc
     2881 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag
     2941 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     3001 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     3061 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     3121 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     3181 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     3241 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     3301 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     3361 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     3421 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     3481 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     3541 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     3601 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     3661 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     3721 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     3781 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     3841 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     3901 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     3961 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     4021 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     4081 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     4141 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     4201 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     4261 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     4321 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     4381 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     4441 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     4501 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     4561 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     4621 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     4681 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     4741 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     4801 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     4861 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     4921 gaggaagcgg aaga
//
