LOCUS       .                       4420 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     protein_bind    106..127
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        142..176
                     /label="J23119(SpeI) promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     184..200
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             221..238
                     /label="6xHis"
                     /note="6xHis affinity tag"
     primer_bind     complement(1787..1803)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      2054..2140
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2232..2259
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2514..2618
                     /label="AmpR promoter"
     CDS             2619..3476
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3650..4238
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg cttgacagct agctcagtcc taggtataat actagttttc
      181 acacaggaaa cagctatgac catgttacgc caagcttatg catcatcatc atcatcacat
      241 gtcgcaagat gcgaaacaag actttggtaa gtggagggca ttcttttggc ctgtacacgg
      301 ttatgaactt aaaaaacttc ttccaatgtt ttttatgttc ttttttattt ctttcaatta
      361 cacaatttta agagatacaa aagacacttt aatcgtgacc tctgcaggtg cggaagccat
      421 tccatttttg aagtcttttg gtgttgtccc tgcagctatc ctttttatga tcatttatgc
      481 caagcttagc aatacgctaa gcagagaaaa tttattttac gttacgcttt taccttttat
      541 cattttcttc ggcttatttg cttttgtcat gtatccagct agagaagttt taatgcctca
      601 tgcttctgct gaagctttaa aagcttattt acctggcggt tggaccgggc ttgcggcggc
      661 atatgaaaac tggatgtatt cgatttttta cattttagct gaactttggg gaagcgttgt
      721 attatctctt cttttttggg gatttgctaa ccaaattacc cgtgtaaatg aagctaaacg
      781 tttttatagc cttttcggtt tgggtgctaa tttggcactt ttagtttcag gtcctgctat
      841 tgtatatgta tcagatattc gcaagcattt acctgcaaat gttgatgcat ggcaaatttc
      901 tttaaattat ttaatgggta tggtcgtcat tgccggatta gcaattttag ctatctattg
      961 gtggattaat cgtgcagttt taacagatcc acgtttttat gatttaaatc aagaaaaagc
     1021 tcctggtaaa aagaaaaaag ctaaaatgtc tcttggagaa agcttcaaat tcctctttac
     1081 ctctaagtat atcctttgtt tggcgatatt ggttattgct tacggtattt ctattaactt
     1141 agtagaaatt acatggaaga gcctggtgaa acttcaatat cctaatccaa atgattacag
     1201 cacgttcatg ggatggttct caacaatgac gggtgccgtc acaattttga tgatgttatt
     1261 cgttggtggt aacgtaattc gtcacaaagg ttggggattt gcagctttaa ttactccagt
     1321 tgttttatta gtcaccggga ttgctttttt ctcattcgtg atttttaaag atcacctagc
     1381 tggatatatt gctgccttag gaactactcc actattctta gctgtcatct ttggtgcggc
     1441 tcaaaatatt atgagcaaat ctgctaaata ctctttattt gatccaacaa aagaaatggc
     1501 ttatattcct ttagatgatg aatctaaagt aaaaggtaaa gccgcagttg acgtagtagg
     1561 agctcgttta ggaaaatctg gcggatctat tattcaaatg ggattacttg catttggaac
     1621 gttagcaact attacacctt atatcggcgc gattttgatg gttattattg ctgcctggat
     1681 tgtggctgcc cgttcattaa gcaaacaatt tactcaattg actgctgagc agaatattga
     1741 gaaaaatatt gataaaacat cggaaatagc tactagctaa gaattcactg gccgtcgttt
     1801 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     1861 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     1921 tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg catctgtgcg
     1981 gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     2041 tttttccagg catcaaataa aacgaaagtc tcagtcgaaa gactgggcct ttcgttttat
     2101 ctgttgtttg tcggtgaacg ctctcctgag taggacaaat ccgccgggag cggatttgaa
     2161 cgttgcgaag caacggcccg gagggtggcg ggcaggacgc ccgccataaa ctgccaggca
     2221 tcaaattaag cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactctgcc
     2281 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     2341 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     2401 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     2461 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     2521 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     2581 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     2641 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     2701 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     2761 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     2821 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     2881 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     2941 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     3001 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     3061 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     3121 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     3181 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     3241 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     3301 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     3361 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     3421 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     3481 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     3541 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     3601 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     3661 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     3721 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     3781 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     3841 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     3901 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     3961 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     4021 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     4081 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     4141 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     4201 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     4261 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     4321 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     4381 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
//
