LOCUS       .                       3599 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        5..552
                     /label="OpIE-2 promoter"
                     /note="strong constitutive baculovirus promoter for insect
                     cell expression"
     CDS             579..1295
                     /label="EGFP"
                     /note="the original enhanced GFP (Yang et al., 1996)"
     CDS             1437..1454
                     /label="6xHis"
                     /note="6xHis affinity tag"
     polyA_signal    1472..1601
                     /label="OpIE-2 poly(A) signal"
                     /note="baculovirus polyadenylation signal"
     rep_origin      complement(1729..2317)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2965..3012
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             3032..3403
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    3470..3604
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggatcatgat gataaacaat gtatggtgct aatgttgctt caacaacaat tctgttgaac
       61 tgtgttttca tgtttgccaa caagcacctt tatactcggt ggcctcccca ccaccaactt
      121 ttttgcactg caaaaaaaca cgcttttgca cgcgggccca tacatagtac aaactctacg
      181 tttcgtagac tattttacat aaatagtcta caccgttgta tacgctccaa atacactacc
      241 acacattgaa cctttttgca gtgcaaaaaa gtacgtgtcg gcagtcacgt aggccggcct
      301 tatcgggtcg cgtcctgtca cgtacgaatc acattatcgg accggacgag tgttgtctta
      361 tcgtgacagg acgccagctt cctgtgttgc taaccgcagc cggacgcaac tccttatcgg
      421 aacaggacgc gcctccatat cagccgcgcg ttatctcatg cgcgtgaccg gacacgaggc
      481 gcccgtcccg cttatcgcgc ctataaatac agcccgcaac gatctggtaa acacagttga
      541 acagcatctg ttcgaattta aagcttggta ccgccaccat ggtgagcaag ggcgaggagc
      601 tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt
      661 tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca
      721 tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg
      781 gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg
      841 ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca
      901 agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg
      961 gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca
     1021 gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga
     1081 tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc
     1141 ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc
     1201 tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg
     1261 ccgggatcac tctcggcatg gacgagctgt acaagggcgg tggaggcagt ggtggaggcg
     1321 gtagcaaggg atccgagctc gaattcacta gtccagtctc gagtctagag ggcccgcggt
     1381 tcgaaggtaa gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggtcatc
     1441 atcaccatca ccattgagtt tatctgacta aatcttagtt tgtattgtca tgttttaata
     1501 caatatgtta tgtttaaata tgtttttaat aaattttata aaataatttc aacttttatt
     1561 gtaacaacat tgtccattta cacactcctt tcaagcgcgt gggatcgatg ctcactcaaa
     1621 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     1681 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     1741 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     1801 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     1861 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     1921 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     1981 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     2041 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     2101 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     2161 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     2221 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     2281 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     2341 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgatgataaa
     2401 caatgtatgg tgctaatgtt gcttcaacaa caattctgtt gaactgtgtt ttcatgtttg
     2461 ccaacaagca cctttatact cggtggcctc cccaccacca acttttttgc actgcaaaaa
     2521 aacacgcttt tgcacgcggg cccatacata gtacaaactc tacgtttcgt agactatttt
     2581 acataaatag tctacaccgt tgtatacgct ccaaatacac taccacacat tgaacctttt
     2641 tgcagtgcaa aaaagtacgt gtcggcagtc acgtaggccg gccttatcgg gtcgcgtcct
     2701 gtcacgtacg aatcacatta tcggaccgga cgagtgttgt cttatcgtga caggacgcca
     2761 gcttcctgtg ttgctaaccg cagccggacg caactcctta tcggaacagg acgcgcctcc
     2821 atatcagccg cgcgttatct catgcgcgtg accggacacg aggcgcccgt cccgcttatc
     2881 gcgcctataa atacagcccg caacgatctg gtaaacacag ttgaacagca tctgttcgaa
     2941 ttaattcgga tctctgcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     3001 gtataatacg actcactata ggagggccac catggccaag ttgaccagtg ccgttccggt
     3061 gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc
     3121 ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat
     3181 cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg
     3241 cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc
     3301 cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga
     3361 cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgaccga cgccgaccaa
     3421 caccgccggt ccgacggcgg cccacgggtc ccaggggggt cgacctcgaa acttgtttat
     3481 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
     3541 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtct
//
