LOCUS V026960 6833 bp DNA circular SYN 01-JAN-1980 DEFINITION synthetic circular DNA. ACCESSION V026960 VERSION V026960 KEYWORDS . SOURCE . ORGANISM . . FEATURES Location/Qualifiers enhancer 61..364 /note="human cytomegalovirus immediate early enhancer" /label="CMV enhancer" promoter 365..568 /note="human cytomegalovirus (CMV) immediate early promoter" /label="CMV promoter" CDS 624..2150 /label="CYP4X1(NM_178033)" /note="CYP4X1(NM_178033)" /gene="CYP4X1" misc_feature 2192..2778 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" /label="IRES2" CDS 2779..3498 /codon_start=1 /note="mammalian codon-optimized" /product="the original enhanced GFP (Yang et al., 1996)" /transl_table=1 /translation="M,V,SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKL TLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFK DDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMV LLEFVTAAGITLGMDELYK*" /label="EGFP" polyA_signal 3621..3742 /note="SV40 polyadenylation signal" /label="SV40 poly(A) signal" rep_origin complement(3749..4204) /direction=LEFT /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" /label="f1 ori" promoter 4231..4335 /gene="bla" /label="AmpR promoter" promoter 4337..4694 /note="SV40 enhancer and early promoter" /label="SV40 promoter" CDS 4729..5523 /codon_start=1 /gene="aph(3')-II (or nptII)" /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin®)" /product="aminoglycoside phosphotransferase from Tn5" /transl_table=1 /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*" /label="NeoR/KanR" polyA_signal 5755..5802 /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" /label="HSV TK poly(A) signal" rep_origin 6131..6719 /direction=RIGHT /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" /label="ori" ORIGIN 1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagcc accatggaat tctcctggct ggagacgcgc tgggcgcggc 661 ccttttacct ggcgttcgtg ttctgcctgg ccctggggct gctgcaggcc attaagctgt 721 acctgcggag gcagcggctg ctgcgggacc tgcgcccctt cccagcgccc cccacccact 781 ggttccttgg gcaccagaag tttattcagg atgataacat ggagaagctt gaggaaatta 841 ttgaaaaata ccctcgtgcc ttccctttct ggattgggcc ctttcaggca tttttctgta 901 tctatgaccc agactatgca aagacacttc tgagcagaac agatcccaag tcccagtacc 961 tgcagaaatt ctcacctcca cttcttggaa aaggactagc ggctctagac ggacccaagt 1021 ggttccagca tcgtcgccta ctaactcctg gattccattt taacatcctg aaagcataca 1081 ttgaggtgat ggctcattct gtgaaaatga tgctggataa gtgggagaag atttgcagca 1141 ctcaggacac aagcgtggag gtctatgagc acatcaactc gatgtctctg gatataatca 1201 tgaaatgcgc tttcagcaag gagaccaact gccagacaaa cagcacccat gatccttatg 1261 caaaagccat atttgaactc agcaaaatca tatttcaccg cttgtacagt ttgttgtatc 1321 acagtgacat aattttcaaa ctcagccctc agggctaccg cttccagaag ttaagccgag 1381 tgttgaatca gtacacagat acaataatcc aggaaagaaa gaaatccctc caggctgggg 1441 taaagcagga taacactccg aagaggaagt accaggattt tctggatatt gtcctttctg 1501 ccaaggatga aagtggtagc agcttctcag atattgatgt acactctgaa gtgagcacat 1561 tcctgttggc aggacatgac accttggcag caagcatctc ctggatcctt tactgcctgg 1621 ctctgaaccc tgagcatcaa gagagatgcc gggaggaggt caggggcatc ctgggggatg 1681 ggtcttctat cacttgggac cagctgggtg agatgtcgta caccacaatg tgcatcaagg 1741 agacgtgccg attgattcct gcagtcccgt ccatttccag agatctcagc aagccactta 1801 ccttcccaga tggatgcaca ttgcctgcag ggatcaccgt ggttcttagt atttggggtc 1861 ttcaccacaa ccctgctgtc tggaaaaacc caaaggtctt tgaccccttg aggttctctc 1921 aggagaattc tgatcagaga cacccctatg cctacttacc attctcagct ggatcaagga 1981 actgcattgg gcaggagttt gccatgattg agttaaaggt aaccattgcc ttgattctgc 2041 tccacttcag agtgactcca gaccccacca ggcctcttac tttccccaac cattttatcc 2101 tcaagcccaa gaatgggatg tatttgcacc tgaagaaact ctctgaatgt taggaattct 2161 gcagtcgacg gtaccgcggg cccgggatcc gcccctctcc ctcccccccc cctaacgtta 2221 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca 2281 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca 2341 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg 2401 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc 2461 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata 2521 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag 2581 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc 2641 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt 2701 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat 2761 gataatatgg ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 2821 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 2881 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 2941 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 3001 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 3061 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 3121 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 3181 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 3241 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 3301 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 3361 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag 3421 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 3481 gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg 3541 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 3601 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca 3661 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 3721 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc 3781 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc 3841 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag 3901 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc 3961 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa 4021 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg 4081 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt 4141 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc 4201 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 4261 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 4321 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt 4381 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 4441 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 4501 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 4561 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 4621 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 4681 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa 4741 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg 4801 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc 4861 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca 4921 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc 4981 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca 5041 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat 5101 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca 5161 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg 5221 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc 5281 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct 5341 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct 5401 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac 5461 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc 5521 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 5581 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 5641 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg 5701 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa 5761 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt 5821 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg 5881 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca 5941 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt 6001 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 6061 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 6121 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 6181 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 6241 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 6301 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 6361 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 6421 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 6481 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 6541 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 6601 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 6661 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 6721 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 6781 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat //