LOCUS       62056_12875        7498 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7498)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7498
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        247..277
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     protein_bind    285..301
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     309..325
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     sig_peptide     371..433
                     /label=OmpA signal peptide
                     /note="signal peptide from the E. coli outer membrane
                     protein OmpA"
     promoter        1805..1882
                     /label=lacI promoter
     CDS             1883..2962
                     /codon_start=1
                     /label=lacI
                     /note="lac repressor"
                     /translation="VKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     protein_bind    2978..2999
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             5064..5252
                     /codon_start=1
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
                     /translation="VTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     misc_feature    5357..5497
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5683..6271)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6445..7302)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7303..7407)
                     /label=AmpR promoter
ORIGIN
        1 ggataaccag aagcaataaa aaatcaaatc ggatttcact atataatctc actttatcta
       61 agatgaatcc gatggaagca tcctgttttc tctcaatttt tttatctaaa acccagcgtt
      121 cgatgcttct ttgagcgaac gatcaaaaat aagtgccttc ccatcaaaaa aatattctca
      181 acataaaaaa ctttgtgtaa tacttgtaac gctacatgga gattaactca atctagctag
      241 agaggcttta cactttatgc ttccggctcg tataatgtgt ggaattgtga gcggataaca
      301 atttcacaca ggaaacagct atgaccatga ttacggattc actggaactc tagataacga
      361 ggcgcaaaaa atgaaaaaga cagctatcgc gattgcagtg gcactggctg gtttcgctac
      421 cgtagcgcag gccggaattc caagcttgga tccggctgag caacgacgtg aacgcaatgc
      481 gttccgacgt tcaggctgct aaagatgacg cagctcgtgc taaccagcgt ctggacaaca
      541 tggctactaa ataccgcaag taatagtacc tgtgaagtga aaaatggcgc acattgtgcg
      601 acattttttt tgtctgccgt ttaccgctac tgcgtcacgc gtaacatatt cccttgctct
      661 ggttcaccat tctgcgctga ctctactgaa ggcgcattgc tggctgcggg agttgctcca
      721 ctgctcaccg aaaccggata ccctgcccga cgatacaacg ctttatcgac taacttctga
      781 tctacagcct tattgtcttt aaattgcgta aagcctgctg gcagtgtgta tggcattgtc
      841 tgaacgttct gctgttcttc tgccgatagt ggtcgatgta cttcaacata acgcatcccg
      901 ttaggctcca cggaatattt caccggttcg ttgatcactt tcaccggcgt tcccgtccgc
      961 acgctggaga acaaggcttt aatatccggt gcattcatgc gaatacaccc tgaactgacg
     1021 cgcaaaccga cgctgtccgg cgcactggta ccatgaatga ggtattcgcc attaccatgc
     1081 gcgaggcgca gtgcgtaacg tcctagcggg ttatttggtc cggcaggaac gactggcggt
     1141 aatttaatgc cacgctccag cgaacgctga cgaatgcctg ccgtaggcgt ccaggttggg
     1201 ttagggattt tctgcccaac acgcgtttcc atcaccggcg tttccagccc ctgcaatcca
     1261 atacctattg gataaacctg cacaatattt tctcccggcg gataataata aaggcgcagc
     1321 tctgcaaggt tggtcgacgg tcgaccggtc tcacttcaac ataacgcatc ccgttaggct
     1381 ccacggaata tttcaccggt tcgttgatca ctttcaccgg cgttcccgtc cgcacgctgg
     1441 agaacaaggc tttaatatcc ggtgcattca tgcgaataca ccctgaactg acgcgcaaac
     1501 cgacgctgtc cggcgcactg gtaccatgaa tgaggtattc gccattacca tgcgcgaggc
     1561 gcagtgcgta acgtcctagc gggttatttg gtccggcagg aacgactggc ggtaatttaa
     1621 tgccacgctc cagcgaacgc tgacgaatgc ctgccgtagg cgtccaggtt gggttaggga
     1681 ttttctgccc aacacgcgtt tccatcaccg gcgtttccag cccctgcaat ccaataccta
     1741 ttggataaac ctgcacaata ttttctcccg gcggataata ataaaggcgc agctctgcaa
     1801 ggttgacacc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga
     1861 gagtcaattc agggtggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc
     1921 cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa
     1981 aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc
     2041 acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct
     2101 gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag
     2161 cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa
     2221 tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc
     2281 cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca
     2341 gacacccatc aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca
     2401 tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc
     2461 ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat
     2521 agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct
     2581 gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc
     2641 aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata
     2701 cgacgatacc gaagacagct catgttatat cccgccgtta accaccatca aacaggattt
     2761 tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt
     2821 gaagggcaat cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa
     2881 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     2941 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     3001 aggcacccca ggctttacac tttatgctaa cgataatccc tgacgcggtg catcaggtaa
     3061 taacagttgt gaaggaatag ttatcgtcgt accaggtttt ggcaccgggg cgatagtgtt
     3121 attggcttca aggatcaaca ttgccgcagt atcaaaacgt cgggcaatag cctgaaggtt
     3181 tttatcccct tcttgcaccg tatacgtttg attttgccca accagtcggc ttccggttgg
     3241 tggtagcgga taatcaaccg cccaggcagc ctggatggcg ctaaaagcgc cgataagcgt
     3301 gagtgtaagc aaagacgcgc gtttcattgt aaacctcctg tatttgccgg agactcacgc
     3361 tgaaacgtcg gatggcgctt atgttcacct gaaaccaaaa cactcctgtg caggtcagtg
     3421 taaacattga ccatccggca atgtgagcca accggatgaa agctgtcctt ttagtttagc
     3481 taagtgcagc ggctttggcg cgaattgcgc gaatcatcgc ttccagacct tgtgaacgag
     3541 atggggtgag atgttgggtg agcgccattt tttcaaacca cggacgcaca tcgaaattga
     3601 caatatcctg cggcgtcatc tgatcgtaga gaataaagac gaccgcaata agccctttca
     3661 caatcgccgc atcgctgtcg ccctgtaatt caataattcc ctgggcattc tggcgcatga
     3721 caatccacac ctgactctga cagccctgaa tgctattttg tggacttctg tcttcgtcgc
     3781 gtaattctgg cagacgctgg gggaccgatg cccttgagag ccttcaaccc agtcagctcc
     3841 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
     3901 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
     3961 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
     4021 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
     4081 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
     4141 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
     4201 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
     4261 cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg
     4321 gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc
     4381 cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga agccggcggc
     4441 acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg cggagaactg
     4501 tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc tccagcagcc
     4561 gcacgcggcg catctcgggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc
     4621 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac
     4681 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa
     4741 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct
     4801 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta
     4861 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca
     4921 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag
     4981 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa
     5041 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc
     5101 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg
     5161 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc
     5221 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
     5281 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
     5341 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg
     5401 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatgc ggtgtgaaat
     5461 accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt cctcgctcac
     5521 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5581 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5641 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5701 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5761 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5821 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5881 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5941 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     6001 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     6061 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     6121 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6181 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6241 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6301 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6361 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6421 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6481 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6541 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6601 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6661 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6721 gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc
     6781 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6841 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6901 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6961 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     7021 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     7081 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     7141 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7201 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7261 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7321 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7381 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     7441 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcccttt
//
