LOCUS       Exported                2980 bp DNA     circular SYN 25-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..2980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    31..259
                     /label=MCS
     polyA_signal    355..579
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(838..1426)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1597..2457)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(2458..2562)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        2726..2929
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        join(2974..2980,1..12)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 actcactata gggagaccca agctgttaac gctagcagtt aaccggagta ctggtcgacc
       61 tccgaagttg ggggggagga gacggtaccg tctccaataa cccggcggcc caaaatgccg
      121 actcggagcg aaagatatac ctcccccggg gccgggaggt cgcgtcaccg accacgccgc
      181 cggcccaggc gacgcgcgac acggacacct gtccccaaaa acgccaccat cgcagccaca
      241 cacggagcgc ccggggccct ctggtcaacc ccaggacaca cgcgggagca gcgccgggcc
      301 ggggacgccc tcccggcggt cacctaaatg ctagagctcg ctgatcagcc tcgactgtgc
      361 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
      421 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
      481 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
      541 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
      601 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      661 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      721 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      781 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      841 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      901 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
      961 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1021 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1081 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1141 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1201 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1261 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1321 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1381 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1441 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1501 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1561 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1621 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1681 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1741 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1801 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     1861 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     1921 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     1981 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2041 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2101 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2161 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2221 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2281 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2341 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     2401 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     2461 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2521 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2581 gccacctgac gtcgatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     2641 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     2701 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     2761 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2821 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2881 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc tctggctaac
     2941 tagagaaccc actgcttact ggcttatcga aattaatacg
//
