LOCUS       .                       9625 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             2973..3707
                     /gene="erm"
                     /label="erm"
                     /note="rRNA adenine N-6-methyltransferase from
                     Streptococcus pneumoniae. Accession#: P21236"
     CDS             3862..4719
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      4893..5481
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     5956..5972
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     CDS             5988..9038
                     /label="lacZ"
                     /note="β-galactosidase"
     primer_bind     complement(9048..9064)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
ORIGIN
        1 ccatcctcca aagttggaga gtgagtttta tgtcgcaaat attaatgttt ctggtgaacc
       61 ttatcaaatt ttcgttgatt taatagaaac atagcggtaa aattagcagt aacttaatag
      121 aacggaaatg aaaaaagcca ctctcatatg ctattggcta ccaaccttta gcgagaatga
      181 cttaatcctg tacagccata caggacttcg acttataaga ggcgccaacc tcaaataagt
      241 tatttgcctt gttttcgcga acaaggctta ttagatacac ctattgtacc gttactctac
      301 gaatatttca actagtaatt actagcattg tcatatacat aataaaacgg atataaaagg
      361 gcgttttcta tacctagaag tcttgtaaat gtacagggcg tttagatata gagaacgccc
      421 tttttgtgtt ccgttccagt ggaggctacc actttaaaaa gatggtctag tgtagccaat
      481 gcaggagagt acactcggat atcagttgtc gttgcattca actgtctgac gtaagcgagg
      541 taaaggacac aagccttgca taaaacaagc ctacgggatg taaatcctaa taatgatgat
      601 aaccaagacg ttagcggcaa aaagtgttgg gggttcaaaa taagacatga ttgtgcgact
      661 ggagttaaac agttactcgt aagcggcgat catgacactg attcacggct attcttgtac
      721 aaactttatt acaaggatat gcgggttata tagcgaatca cccgaaaggg aacggtgttg
      781 ggcgtgagaa acgcaccgta cggcgcaata caatgccaat aagctatata cggacggtat
      841 agtagttttg taagctataa ccgtttgtcg tcaatgcaac caatctcaat tcgagacctc
      901 ggcatctaag ccagtacgaa tgagtgggcg ttttaacctc gtaaattttc aacaggggtt
      961 actatgccca aaactacatt cagatttcct aacaaactcg ccagtatgaa aaccttaaga
     1021 ccttaaagtc aagggatttg aaggatttta acctcgatta gcaaaaaatg tagagtactg
     1081 aagcaactac cattaactaa gatagtgggg gattgaggaa gaatccagag ctgtttaaat
     1141 caagtgaaag acaagatgaa attaaaagaa tagtgaaaga taggggagtg gttctctatg
     1201 agaaaggaaa tggctagaga acaaaggcag cggtttattg atctattgtt agactttatg
     1261 gtaaagaatc ctcatttatt tgttaatggt acagaggatg aaagtaataa tgttgttaca
     1321 aaatgtaata gtgatattaa agaggttgcg gagtcatatt taactctttt atagtgagag
     1381 ggttaaaact aattaatatg tattaaggcc caatgttgga attattgtat ttcactaggc
     1441 aacctactta ctaaaagtaa gattatccat tagtggatgt tataatattg ggttttttaa
     1501 cacaataatc atcgcctttc ggtgtcgttt gatagaaaag taaccattag cgatgaaaaa
     1561 gtcaatataa aaagccatcc gtaaaaaacg gatggcttac cgtacatagg atcgttggta
     1621 gggcggcgta tcctacatct ctggtaactg acctagccaa tcaaatgctt gagaacggcg
     1681 gttagataag cgcgtgggga acctttccca cctcaaagat cctatatcat tattatgtta
     1741 ctttctacag gtagtatacc atgttcttat attttagtaa actccccgtt agcttaacag
     1801 gtctttgtaa gcaattaaac gtccactatt caatcgtctt tggattttcg caggaccgtt
     1861 ttttagatcg aacatagttg ataagaacaa ataaccgctt gggtccaact ttatagcaat
     1921 tagtatatgg tcatttaaaa tctttaccaa ttcaacgcta ttaggttctt taggattttg
     1981 cccgacatag tcggggtgtt caacgatatc ttttatgtgc gatgaatatt tttcataaat
     2041 accaggatgt tgtttcttta cgtgctttat aaatccggga aacattttta catcgttaga
     2101 agtgcaagtc aagttatatg tatctataat gatttgtgga agttttgcca caacagttgg
     2161 tttatttaca atcttttttt tattagccgt caaatttctc cctcatctcg tctctttata
     2221 tctttatttt atcataaagg agtatttgaa ccgtcgcgcg ggacaggttt atgataggga
     2281 tattttattg aataattgat ggtataaggg actttcatgc ttggaaagtg gggattatga
     2341 attagatgct tgtccacaat atgttccaat gtaattaaaa tttatgttcc caccttgacc
     2401 aaacatcacg tccatactta aatcgtccct cctttaatag gtaaaatatt aatttacctt
     2461 aataaaaaaa taatggataa tagtattcgt ctgaatttat ataatcaggg ggaactattg
     2521 atgctgggga tactatttac agcggcgcca tctactgatg tcgtaaagga tttgcaagat
     2581 aaagttatat cattgcagga tcatgaggta gcgtttttga acaccacgat atctaatatg
     2641 ttgatcccct tagaagcaaa cttaagagtg tgttgatagt gcagtatctt aaaattttgt
     2701 gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagggatt
     2761 cgtcatgttg gtattccaaa tgcgtaatgt agataaaaca tctactgttt tgaaacagac
     2821 taaaaacagt gattacgcag ataaataaat acgttagatt aattcctacc agtgactaat
     2881 cttatgactt tttaaacaga taactaaaat tacaaacaaa tcgtttaact tctgtattta
     2941 tttacagatg taatcacttc aggagtaatt acatgaacaa aaatataaaa tattctcaaa
     3001 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
     3061 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
     3121 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgacagaaa
     3181 aattaaaact gaacattcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
     3241 ctaacaaaca gaggtataaa attgttggga gtattcctta ccatttaagc acacaaatta
     3301 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
     3361 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
     3421 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca
     3481 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
     3541 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
     3601 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccatt acttatgagc
     3661 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
     3721 cgctttttta aatttggaaa gttacacgtt actaaaggga atggagataa attattagat
     3781 atactactga cagcttccaa gaaggtaaag aggtccctag cgcctacggg gaatttgtat
     3841 cgggattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     3901 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     3961 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4021 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4081 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4141 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4201 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4261 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4321 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4381 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4441 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4501 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     4561 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     4621 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     4681 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     4741 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     4801 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     4861 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     4921 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     4981 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     5041 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5101 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5161 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     5221 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     5281 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     5341 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     5401 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     5461 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     5521 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     5581 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     5641 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     5701 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     5761 aacgcaatag caaaaggaac ttcagatgaa gttcctttaa atttttctaa tttagtatat
     5821 gtgaatgatt ttttaacttt acttctatgc aatatttcat attgatgaaa tgttatttat
     5881 tttttatgaa ctagcttata ttatattcaa taagtttgat gtaaaaacag aaaaagtgag
     5941 ggataatatt tcacacagga aacagctatg accatgatta cgccaagctt atggtcgttt
     6001 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     6061 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     6121 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
     6181 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc
     6241 agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt acggtcaatc
     6301 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg
     6361 aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc
     6421 atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat
     6481 ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgct
     6541 ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg
     6601 acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct
     6661 ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc
     6721 gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca
     6781 ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac
     6841 tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg
     6901 cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg
     6961 gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga
     7021 ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga
     7081 cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc
     7141 attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg
     7201 atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc
     7261 gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc
     7321 cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc
     7381 tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag
     7441 ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc
     7501 ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc
     7561 gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta
     7621 aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg
     7681 tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg
     7741 attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc
     7801 gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt
     7861 tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc
     7921 tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg
     7981 atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg
     8041 ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag
     8101 ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc
     8161 ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc
     8221 tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg
     8281 gcgataaaaa ccaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata
     8341 acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga
     8401 aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg
     8461 ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta
     8521 tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg
     8581 aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg
     8641 tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta
     8701 ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct
     8761 tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt
     8821 ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca
     8881 gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata
     8941 tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattc cagctgagcg
     9001 ccggtcgcta ccattaccag ttggtctggt gtcaaaaata agaattcact ggccgtcgtt
     9061 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     9121 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     9181 ttgcgcagcc tgaatggcga atggcgcctg atgcggtatt ttctccttac gcatctgtgc
     9241 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     9301 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     9361 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     9421 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     9481 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     9541 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     9601 taaccctgat aaatgcttca ataat
//
