LOCUS       62056_12355        5065 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5065)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             562..579
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     polyA_signal    756..811
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1172..1188)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1196..1212)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1220..1250)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1265..1286)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1583..2171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2345..3202)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3203..3307)
                     /label=AmpR promoter
     intron          3997..5014
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 ttgcggccgt ctcaggccga attcaagctt gccaccatgg ggatccttcc cagccctggg
       61 atgcctgcgc tgctctccct cgtgagcctt ctctccgtgc tgctgatggg ttgcgtagct
      121 gaaaccggtt cgatagtttt agagcctatc tattggaatt cctcgaactc caaatttcta
      181 cctggacaag gactggtact atacccacag ataggagaca aattggatat tatttgcccc
      241 aaagtggact ctaaaactgt tggccagtat gaatattata aagtttatat ggttgataaa
      301 gaccaagcag acagatgcac tattaagaag gaaaataccc ctctcctcaa ctgtgccaaa
      361 ccggaccaag atatcaaatt caccatcaag tttcaagaat tcagccctaa cctctggggt
      421 ctagaatttc agaagaacaa agattattac attatatcta catcaaatgg gtctttggag
      481 ggcctggata accaggaggg aggggtgtgc cagacaagag ccatgaagat cctcatgaaa
      541 gttggacaag atggtaccaa gcaccaccat caccatcact aatgatcact cgagactagt
      601 atcgcgataa ttcactcctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc
      661 caatgccctg gctcacaaat accactgaga tctttttccc tctgccaaaa attatgggga
      721 catcatgaag ccccttgagc atctgacttc tggctaataa aggaaattta ttttcattgc
      781 aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga gggcaaatca
      841 tttaaaacat cagaatgagt atttggttta gagtttggca acatatgccc atatgctggc
      901 tgccatgaac aaaggttggc tataaagagg tcatcagtat atgaaacagc cccctgctgt
      961 ccattcctta ttccatagaa aagccttgac ttgaggttag atttttttta tattttgttt
     1021 tgtgttattt ttttctttaa catccctaaa attttcctta catgttttac tagccagatt
     1081 tttcctcctc tcctgactac tcccagtcat agctgtccct cttctcttat ggagatccct
     1141 cgacctgcag cccaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     1201 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     1261 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     1321 gaaacctgtc gtgccagcgg atcccgctgc attaatgaat cggccaacgc gcggggagag
     1381 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1441 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1501 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1561 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1621 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1681 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1741 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1801 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1861 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1921 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1981 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     2041 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     2101 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     2161 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2221 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2281 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2341 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2401 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2461 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2521 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2581 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2641 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2701 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2761 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2821 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2881 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2941 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     3001 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     3061 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     3121 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3181 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     3241 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     3301 ttccgcgcac atttccccga aaagtgccac ctgggtcgac attgattatt gactagttat
     3361 taatagtaat caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca
     3421 taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca
     3481 ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg
     3541 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
     3601 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc
     3661 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
     3721 gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc acccccaatt
     3781 ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg gggggggggg
     3841 ccccccccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg gaaaggtgcg
     3901 gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag gcggcggcgg
     3961 cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgttgcgcg ctgccttccc
     4021 cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta
     4081 ctcccacagg tgagcgggcg ggacggccct tctcctccgg gctgtaatta gcgcttggtt
     4141 taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc
     4201 cctttgtgcg gggggagcgg ctcggggggt gcgtgcgtgt gtgtgtgcgt ggggagcgcc
     4261 gcgtgcggct ccgcgctgcc cggcggctgt gagcgctgcg ggcgcggcgc ggggctttgt
     4321 gcgctccgca gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg gtgcgggggg
     4381 ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg tgggggggtg agcagggggt
     4441 gtgggcgcgt cggtcgggct gcaacccccc ctgcaccccc ctccccgagt tgctgagcac
     4501 ggcccggctt cgggtgcggg gctccgtacg gggcgtggcg cggggctcgc cgtgccgggc
     4561 ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc cggggagggc
     4621 tcgggggaag gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg
     4681 cagccattgc cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat
     4741 ctgtgcggag ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa
     4801 gcggtgcggc gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg
     4861 ccgtcccctt ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg
     4921 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     4981 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     5041 gctgtctcat cattttggca aagaa
//
