LOCUS       V005558                 8819 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005558
VERSION     V005558
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   De Schamphelaire W, Olbrechts A, Meert J, Verhelst K, Roggeman
            Fonseca M, Vanhoucke M, Beyaert R.
  TITLE     BCCM/LMBP Plasmid collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8819)
  AUTHORS   De Schamphelaire W.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2017) BCCM/LMBP, Universiteit Gent,
            Technologiepark 927, 9052, BELGIUM
REFERENCE   3  (bases 1 to 8819)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 8819)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (02-FEB-2017) BCCM/LMBP, Universiteit Gent, Technologiepark 927,
            9052, BELGIUM"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8819
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..974
                     /label="AOX1 promoter"
                     /note="inducible promoter, regulated by methanol"
     CDS             990..1565
                     /gene="Bax"
                     /label="Apoptosis regulator BAX"
                     /note="Apoptosis regulator BAX from Mus musculus.
                     Accession#: Q07813"
     terminator      1662..1908
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     CDS             complement(2321..4855)
                     /codon_start=1
                     /note="unnamed protein product; Pichia HIS4"
                     /protein_id="SJL86635.1"
                     /translation="MTFPLLPAYASVAEFDNSLSLVGKAVFPYAADQLHNLIKFTQSTE
                     LQVNVQVESSVTEDQFEELIDNLLKLYNNGINEVILDLDLAERVVQRMIPGARVIYRTL
                     VDKVASLPANASIAVPFSSPLGDLKSFTNGGSRTVYAFSETAKLVDVTSTVASGIIPII
                     DARQLTTEYELSEDVKKFPVSEILLASLTTDRPDGLFTTLVADSSNYSLGLVYSSKKSI
                     PEAIRTQTGVYQSRRHGLWYKGATSGATQKLLGIELDCDGDCLKFVVEQTGVGFCHLER
                     TSCFGQSKGLRAMEATLWDRKSNAPEGSYTKRLFDDEVLLNAKIREEADELAEAKSKED
                     IAWECADLFYFALVRCAKYGVTLDEVERNLDMKSLKVTRRKGDAKPGYTKEQPKEESKP
                     KEVPSEGRIELCKIDVSKASSQEIEDALRRPIQKTEQIMELVKPIVDNVRQNGDKALLE
                     LTAKFDGVALKTPVLEAPFPEELMQLPDNVKRAIDLSIDNVRKFHEAQLTETLQVETCP
                     GVVCSRFARPIEKVGLYIPGGTAILPSTSLMLGVPAKVAGCKEIVFASPPKKDGTLTPE
                     VIYVAHKVGAKCIVLAGGAQAVAAMAYGTETVPKCDKIFGPGNQFVTAAKMMVQNDTSA
                     LCSIDMPAGPSEVLVIADKYADPDFVASDLLSQAEHGIDSQVILLAVDMTDKELARIED
                     AVHNQAVQLPRVEIVRKCIAHSTTLSVATYEQALEMSNQYAPEHLILQIENASSYVDQV
                     QHAGSVFVGAYSPESCGDYSSGTNHTLPTYGYARQYSGVNTATFQKFITSQDVTPEGLK
                     HIGQAVMDLAAVEGLDAHRNAVKVRMEKLGLI"
     misc_feature    5210..5966
                     /label="AOX1 3' fragment"
                     /note="region downstream of Pichia pastoris AOX1 gene"
     promoter        6213..6317
                     /label="AmpR promoter"
     CDS             6318..7175
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      complement(7220..7675)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     rep_origin      7786..8374
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     misc_feature    complement(8560..8700)
                     /label="bom"
                     /note="basis of mobility region from pBR322"
ORIGIN
        1 atcaccgaaa cgcgcgaggc agagatcgcg gccgcgatct aacatccaaa gacgaaaggt
       61 tgaatgaaac ctttttgcca tccgacatcc acaggtccat tctcacacat aagtgccaaa
      121 cgcaacagga ggggatacac tagcagcaga ccgttgcaaa cgcaggacct ccactcctct
      181 tctcctcaac acccactttt gccatcgaaa aaccagccca gttattgggc ttgattggag
      241 ctcgctcatt ccaattcctt ctattaggct actaacacca tgactttatt agcctgtcta
      301 tcctggcccc cctggcgagg tcatgtttgt ttatttccga atgcaacaag ctccgcatta
      361 cacccgaaca tcactccaga tgagggcttt ctgagtgtgg ggtcaaatag tttcatgttc
      421 ccaaatggcc caaaactgac agtttaaacg ctgtcttgga acctaatatg acaaaagcgt
      481 gatctcatcc aagatgaact aagtttggtt cgttgaaatg ctaacggcca gttggtcaaa
      541 aagaaacttc caaaagtcgc cataccgttt gtcttgtttg gtattgattg acgaatgctc
      601 aaaaataatc tcattaatgc ttagcgcagt ctctctatcg cttctgaacc cggtggcacc
      661 tgtgccgaaa cgcaaatggg gaaacaaccc gctttttgga tgattatgca ttgtcctcca
      721 cattgtatgc ttccaagatt ctggtgggaa tactgctgat agcctaacgt tcatgatcaa
      781 aatttaactg ttctaacccc tacttgacag caatatataa acagaaggaa gctgccctgt
      841 cttaaacctt tttttttatc atcattatta gcttactttc ataattgcga ctggttccaa
      901 ttgacaagct tttgatttta acgactttta acgacaactt gagaagatca aaaaacaact
      961 aattattcga aacgaggaat tctagagtca tggacgggtc cggggagcag cttgggagcg
     1021 gcgggcccac cagctctgaa cagatcatga agacaggggc ctttttgcta cagggtttca
     1081 tccaggatcg agcagggagg atggctgggg agacacctga gctgaccttg gagcagccgc
     1141 cccaggatgc gtccaccaag aagctgagcg agtgtctccg gcgaattgga gatgaactgg
     1201 atagcaatat ggagctgcag aggatgattg ctgacgtgga cacggactcc ccccgagagg
     1261 tcttcttccg ggtggcagct gacatgtttg ctgatggcaa cttcaactgg ggccgcgtgg
     1321 ttgccctctt ctactttgct agcaaactgg tgctcaaggc cctgtgcact aaagtgcccg
     1381 agctgatcag aaccatcatg ggctggacac tggacttcct ccgtgagcgg ctgcttgtct
     1441 ggatccaaga ccagggtggc tgggaaggcc tcctctccta cttcgggacc cccacatggc
     1501 agacagtgac catctttgtg gctggagtcc tcaccgcctc gctcaccatc tggaagaaga
     1561 tgggctgaga cctgcaggca tgcaagctaa ttcgccttag acatgactgt tcctcagttc
     1621 aagttgggca ttacgagaag accggtcttg ctagattcta atcaagagga tgtcagaatg
     1681 ccatttgcct gagagatgca ggcttcattt ttgatacttt tttatttgta acctatatag
     1741 tataggattt tttttgtcat tttgtttctt ctcgtacgag cttgctcctg atcagcctat
     1801 ctcgcagctg atgaatatct tgtggtaggg gtttgggaaa atcattcgag tttgatgttt
     1861 ttcttggtat ttcccactcc tcttcagagt acagaagatt aagtgagaag ttcgtttgtg
     1921 caagcttatc gataagcttt aatgcggtag tttatcacag ttaaattgct aacgcagtca
     1981 ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg tcaccctgga
     2041 tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg atatcgtcca
     2101 ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt tgatgcaatt
     2161 tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc cagtcctgct
     2221 cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac ccgtcctgtg
     2281 gatctatcga atctaaatgt aagttaaaat ctctaaataa ttaaataagt cccagtttct
     2341 ccatacgaac cttaacagca ttgcggtgag catctagacc ttcaacagca gccagatcca
     2401 tcactgcttg gccaatatgt ttcagtccct caggagttac gtcttgtgaa gtgatgaact
     2461 tctggaaggt tgcagtgtta actccgctgt attgacgggc atatccgtac gttggcaaag
     2521 tgtggttggt accggaggag taatctccac aactctctgg agagtaggca ccaacaaaca
     2581 cagatccagc gtgttgtact tgatcaacat aagaagaagc attctcgatt tgcaggatca
     2641 agtgttcagg agcgtactga ttggacattt ccaaagcctg ctcgtaggtt gcaaccgata
     2701 gggttgtaga gtgtgcaata cacttgcgta caatttcaac ccttggcaac tgcacagctt
     2761 ggttgtgaac agcatcttca attctggcaa gctccttgtc tgtcatatcg acagccaaca
     2821 gaatcacctg ggaatcaata ccatgttcag cttgagacag aaggtctgag gcaacgaaat
     2881 ctggatcagc gtatttatca gcaataacta gaacttcaga aggcccagca ggcatgtcaa
     2941 tactacacag ggctgatgtg tcattttgaa ccatcatctt ggcagcagta acgaactggt
     3001 ttcctggacc aaatattttg tcacacttag gaacagtttc tgttccgtaa gccatagcag
     3061 ctactgcctg ggcgcctcct gctagcacga tacacttagc accaaccttg tgggcaacgt
     3121 agatgacttc tggggtaagg gtaccatcct tcttaggtgg agatgcaaaa acaatttctt
     3181 tgcaaccagc aactttggca ggaacaccca gcatcaggga agtggaaggc agaattgcgg
     3241 ttccaccagg aatatagagg ccaactttct caataggtct tgcaaaacga gagcagacta
     3301 caccagggca agtctcaact tgcaacgtct ccgttagttg agcttcatgg aatttcctga
     3361 cgttatctat agagagatca atggctctct taacgttatc tggcaattgc ataagttcct
     3421 ctgggaaagg agcttctaac acaggtgtct tcaaagcgac tccatcaaac ttggcagtta
     3481 gttctaaaag ggctttgtca ccattttgac gaacattgtc gacaattggt ttgactaatt
     3541 ccataatctg ttccgttttc tggataggac gacgaagggc atcttcaatt tcttgtgagg
     3601 aggccttaga aacgtcaatt ttgcacaatt caatacgacc ttcagaaggg acttctttag
     3661 gtttggattc ttctttaggt tgttccttgg tgtatcctgg cttggcatct cctttccttc
     3721 tagtgacctt tagggacttc atatccaggt ttctctccac ctcgtccaac gtcacaccgt
     3781 acttggcaca tctaactaat gcaaaataaa ataagtcagc acattcccag gctatatctt
     3841 ccttggattt agcttctgca agttcatcag cttcctccct aattttagcg ttcaacaaaa
     3901 cttcgtcgtc aaataaccgt ttggtataag aaccttctgg agcattgctc ttacgatccc
     3961 acaaggtggc ttccatggct ctaagaccct ttgattggcc aaaacaggaa gtgcgttcca
     4021 agtgacagaa accaacacct gtttgttcaa ccacaaattt caagcagtct ccatcacaat
     4081 ccaattcgat acccagcaac ttttgagttg ctccagatgt agcaccttta taccacaaac
     4141 cgtgacgacg agattggtag actccagttt gtgtccttat agcctccgga atagactttt
     4201 tggacgagta caccaggccc aacgagtaat tagaagagtc agccaccaaa gtagtgaata
     4261 gaccatcggg gcggtcagta gtcaaagacg ccaacaaaat ttcactgaca gggaactttt
     4321 tgacatcttc agaaagttcg tattcagtag tcaattgccg agcatcaata atggggatta
     4381 taccagaagc aacagtggaa gtcacatcta ccaactttgc ggtctcagaa aaagcataaa
     4441 cagttctact accgccatta gtgaaacttt tcaaatcgcc cagtggagaa gaaaaaggca
     4501 cagcgatact agcattagcg ggcaaggatg caactttatc aaccagggtc ctatagataa
     4561 ccctagcgcc tgggatcatc ctttggacaa ctctttctgc caaatctagg tccaaaatca
     4621 cttcattgat accattattg tacaacttga gcaagttgtc gatcagctcc tcaaattggt
     4681 cctctgtaac ggatgactca acttgcacat taacttgaag ctcagtcgat tgagtgaact
     4741 tgatcaggtt gtgcagctgg tcagcagcat agggaaacac ggcttttcct accaaactca
     4801 aggaattatc aaactctgca acacttgcgt atgcaggtag caagggaaat gtcatacttg
     4861 aagtcggaca gtgagtgtag tcttgagaaa ttctgaagcc gtatttttat tatcagtgag
     4921 tcagtcatca ggagatcctc tacgccggac gcatcgtggc cggcatcacc ggcgccacag
     4981 gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg gctcgccact
     5041 tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg gccgggggac
     5101 tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc aacggcctca
     5161 acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt cgagtatcta
     5221 tgattggaag tatgggaatg gtgatacccg cattcttcag tgtcttgagg tctcctatca
     5281 gattatgccc aactaaagca accggaggag gagatttcat ggtaaatttc tctgactttt
     5341 ggtcatcagt agactcgaac tgtgagacta tctcggttat gacagcagaa atgtccttct
     5401 tggagacagt aaatgaagtc ccaccaataa agaaatcctt gttatcagga acaaacttct
     5461 tgtttcgaac tttttcggtg ccttgaacta taaaatgtag agtggatatg tcgggtagga
     5521 atggagcggg caaatgctta ccttctggac cttcaagagg tatgtagggt ttgtagatac
     5581 tgatgccaac ttcagtgaca acgttgctat ttcgttcaaa ccattccgaa tccagagaaa
     5641 tcaaagttgt ttgtctacta ttgatccaag ccagtgcggt cttgaaactg acaatagtgt
     5701 gctcgtgttt tgaggtcatc tttgtatgaa taaatctagt ctttgatcta aataatcttg
     5761 acgagccaag gcgataaata cccaaatcta aaactctttt aaaacgttaa aaggacaagt
     5821 atgtctgcct gtattaaacc ccaaatcagc tcgtagtctg atcctcatca acttgagggg
     5881 cactatcttg ttttagagaa atttgcggag atgcgatatc gagaaaaagg tacgctgatt
     5941 ttaaacgtga aatttatctc aagatcgcgg ccgcgatctc gaataataac tgttattttt
     6001 cagtgttccc gatctgcgtc tatttcacaa taccaacatg agtcagctta tcgatgataa
     6061 gctgtcaaac atgagaatta attcgatgat aagctgtcaa acatgagaaa tcttgaagac
     6121 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     6181 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     6241 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     6301 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     6361 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     6421 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     6481 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     6541 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact
     6601 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6661 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6721 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6781 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6841 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6901 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6961 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     7021 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     7081 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     7141 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     7201 atatacttta gattgattta aattgtaaac gttaatattt tgttaaaatt cgcgttaaat
     7261 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     7321 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     7381 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     7441 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     7501 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     7561 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     7621 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtaaaag
     7681 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     7741 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     7801 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     7861 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     7921 accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     7981 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     8041 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     8101 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     8161 atacctacag cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     8221 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     8281 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     8341 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     8401 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     8461 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     8521 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     8581 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     8641 tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg
     8701 ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc
     8761 gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtc
//
