LOCUS       .                       4524 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             complement(443..2092)
                     /label="luciferase"
                     /note="firefly luciferase"
     terminator      2494..2580
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2672..2699
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     promoter        2718..2809
                     /label="AmpR promoter"
     CDS             2810..3667
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      3841..4429
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 gatcacgatc ctttctggcg agtccccgtg cggagtcgga gagcgctccc tgagcgcgcg
       61 tgcggcccga gaggtcgcgc ctggccggcc ttcggtccct cgtgtgtccc ggtcgtagga
      121 ggggcggccg aaaatgcttc cggctcccgc tctggagaca cgggccggcc ccctgcgtgt
      181 ggcacgggcg gccgggaggg cgtccccggc ccggcgctgc tcccgcgtgt gtcctggggt
      241 tgaccagagg gccccgggcg ctccgtgtgt ggctgcgatg gtggcgtttt tggggacagg
      301 tgtccgtgtc gcgcgtcgcc tgggccggcg gcgtggtcgg tgacgcgacc tcccggcccc
      361 gggggaggta tatctttcgc tccgagtcgg cattttgggc cgccgggtta ttagtagaaa
      421 caagggtgtt ttttatcatt tacacggcga tctttccgcc cttcttggcc tttatgagga
      481 tctctctgat ttttcttgcg tcgagttttc cggtaagacc tttcggtact tcgtccacaa
      541 acacaactcc tccgcgcaac tttttcgcgg ttgttacttg actggcgacg taatccacga
      601 tctctttttc cgtcatcgtc tttccgtgct ccaaaacaac aacggcggcg ggaagttcac
      661 cggcgtcatc gtcgggaaga cctgcgacac ctgcgtcgaa gatgttgggg tgttggagca
      721 agatggattc caattcagcg ggagccacct gatagccttt gtacttaatc agagacttca
      781 ggcggtcaac gatgaagaag tgttcgtctt cgtcccagta agctatgtct ccagaatgta
      841 gccatccatc cttgtcaatc aaggcgttgg tcgcttccgg attgtttaca taaccggaca
      901 taatcatagg acctctcaca cacagttcgc ctctttgatt aacgcccagc gttttcccgg
      961 tatccagatc cacaaccttc gcttcaaaaa atggaacaac tttaccgacc gcgcccggtt
     1021 tatcatcccc ctcgggtgta atcagaatag ctgatgtagt ctcagtgagc ccatatcctt
     1081 gcctgatacc tggcagatgg aacctcttgg caaccgcttc cccgacttcc ttagagaggg
     1141 gagcgccacc agaagcaatt tcgtgtaaat tagataaatc gtatttgtca atcagagtgc
     1201 ttttggcgaa gaaggagaat agggttggca ccagcagcgc actttgaatc ttgtaatcct
     1261 gaaggctcct cagaaacagc tcttcttcaa atctatacat taagacgact cgaaatccac
     1321 atatcaaata tccgagtgta gtaaacattc caaaaccgtg atggaatgga acaacactta
     1381 aaatcgcagt atccggaatg atttgattgc caaaaatagg atctctggca tgcgagaatc
     1441 tcacgcaggc agttctatga ggcagagcga cacctttagg cagaccagta gatccagagg
     1501 agttcatgat cagtgcaatt gtcttgtccc tatcgaagga ctctggcaca aaatcgtatt
     1561 cattaaaacc gggaggtaga tgagatgtga cgaacgtgta catcgactga aatccctggt
     1621 aatccgtttt agaatccatg ataataattt tttggatgat tgggagcttt ttttgcacgt
     1681 tcaaaatttt ttgcaacccc tttttggaaa cgaacaccac ggtaggctgc gaaatgccca
     1741 tactgttgag caattcacgt tcattataaa tgtcgttcgc gggcgcaact gcaactccga
     1801 taaataacgc gcccaacacc ggcataaaga attgaagaga gttttcactg catacgacga
     1861 ttctgtgatt tgtattcagc ccatatcgtt tcatagcttc tgccaaccga acggacattt
     1921 cgaagtactc agcgtaagtg atgtccacct cgatatgtgc atctgtaaaa gcaattgttc
     1981 caggaaccag ggcgtatctc ttcatagcct tatgcagttg ctctccagcg gttccatctt
     2041 ccagcggata gaatggcgcc gggcctttct ttatgttttt ggcgtcttcc attatgtctt
     2101 tgtcaccctg cttttgctcc cccccaactt cggaggtcga ccagtactcc gggcgacact
     2161 ttgttttttt ttttcccccg atgctggagg tcgaccagat gtccgaaagt gtcccccccc
     2221 cccccccggc gcggagcggc ggggccactc tggactcttt tttttttttt ttggggatcg
     2281 gccgctagct tggctgtttt tgcggatgag agaagatttt cagcctgata cagattaaat
     2341 cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc gcggtggtcc
     2401 cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt agtgtggggt
     2461 ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc tcagtcgaaa
     2521 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag taggacaaat
     2581 ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg ggcaggacgc
     2641 ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga tggccttttt
     2701 gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     2761 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     2821 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     2881 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     2941 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     3001 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc
     3061 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     3121 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     3181 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     3241 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     3301 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     3361 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     3421 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     3481 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     3541 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     3601 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     3661 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     3721 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     3781 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     3841 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     3901 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     3961 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     4021 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     4081 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     4141 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     4201 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     4261 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     4321 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     4381 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     4441 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     4501 gttatcccct gattcattaa tgca
//
