LOCUS       62056_12285        4539 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4539
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(661..677)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      890..1345
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1627..1731
                     /label=AmpR promoter
     CDS             1732..2589
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2763..3351
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    3639..3660
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        3675..3705
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3713..3729
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3737..3753
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     sig_peptide     3805..3870
                     /label=pelB signal sequence
                     /note="leader peptide for secretion"
     CDS             3916..3945
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
ORIGIN
        1 gaggatccat tcgtttgtga atatcaaggc caatcgtctg acctgcctca acctcctgtc
       61 aatgctggcg gcggctctgg tggtggttct ggtggcggct ctgagggtgg cggctctgag
      121 ggtggcggct ctgagggtgg cggttctgag ggtggcggct ctgagggtgg cggttccggt
      181 ggcggctccg gttccggtga ttttgattat gaaaaaatgg caaacgctaa taagggggct
      241 atgaccgaaa atgccgatga aaacgcgcta cagtctgacg ctaaaggcaa acttgattct
      301 gtcgctactg attacggtgc tgctatcgat ggtttcattg gtgacgtttc cggccttgct
      361 aatggtaatg gtgctactgg tgattttgct ggctctaatt cccaaatggc tcaagtcggt
      421 gacggtgata attcaccttt aatgaataat ttccgtcaat atttaccttc tttgcctcag
      481 tcggttgaat gtcgccctta tgtctttggc gctggtaaac catatgaatt ttctattgat
      541 tgtgacaaaa taaacttatt ccgtggtgtc tttgcgtttc ttttatatgt tgccaccttt
      601 atgtatgtat tttcgacgtt tgctaacata ctgcgtaata aggagtctta ataagaattc
      661 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
      721 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
      781 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
      841 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
      901 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
      961 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     1021 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     1081 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
     1141 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     1201 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
     1261 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     1321 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
     1381 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
     1441 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
     1501 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
     1561 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
     1621 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     1681 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     1741 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     1801 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
     1861 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
     1921 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
     1981 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
     2041 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
     2101 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
     2161 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
     2221 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
     2281 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
     2341 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
     2401 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
     2461 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
     2521 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     2581 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     2641 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     2701 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2761 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2821 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2881 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     2941 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     3001 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     3061 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     3121 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     3181 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     3241 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3301 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3361 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3421 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3481 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3541 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3601 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     3661 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     3721 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgcatgcaaa
     3781 ttctatttca aggagacagt cataatgaaa tacctattgc ctacggcagc cgctggattg
     3841 ttattactcg cggcccagcc ggccatggcc caggtgcagc tgcaggtcga cctcgagatc
     3901 aaacgggcgg ccgcagaaca aaaactcatc tcagaagagg atctgaatgg ggccgcatag
     3961 actgttgaaa gttgtttagc aaaacctcat acagaaaatt catttactaa cgtctggaaa
     4021 gacgacaaaa ctttagatcg ttacgctaac tatgagggct gtctgtggaa tgctacaggc
     4081 gttgtggttt gtactggtga cgaaactcag tgttacggta catgggttcc tattgggctt
     4141 gctatccctg aaaatgaggg tggtggctct gagggtggcg gttctgaggg tggcggttct
     4201 gagggtggcg gtactaaacc tcctgagtac ggtgatacac ctattccggg ctatacttat
     4261 atcaaccctc tcgacggcac ttatccgcct ggtactgagc aaaaccccgc taatcctaat
     4321 ccttctcttg aggagtctca gcctcttaat actttcatgt ttcagaataa taggttccga
     4381 aataggcagg gtgcattaac tgtttatacg ggcactgtta ctcaaggcac tgaccccgtt
     4441 aaaacttatt accagtacac tcctgtatca tcaaaagcca tgtatgacgc ttactggaac
     4501 ggtaaattca gagactgcgc tttccattct ggctttaat
//
