LOCUS       .                       7749 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        113..492
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        493..696
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          808..1380
                     /label="β-globin intron"
                     /note="intron from rabbit β-globin gene"
     CDS             2398..3327
                     /label="emiRFP670"
                     /note="emiRFP670 is a basic (constitutively fluorescent)
                     near ir fluorescent protein published in 2020, derived from
                     Rhodopseudomonas palustris. It has low acid sensitivity. It
                     requires the cofactor biliverdin for fluorescence."
     polyA_signal    4535..4590
                     /label="β-globin poly(A) signal"
                     /note="rabbit β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(4948..4964)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4972..4988)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4996..5026)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5041..5062)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5350..5938)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(6112..6969)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(6970..7074)
                     /label="AmpR promoter"
     rep_origin      complement(7100..7555)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7697..7713
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7723..7741
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctccacc gcggtggcgg ccgctctaga gagcttggcc cattgcatac gttgtatcca
       61 tatcataata tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga
      121 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      181 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      241 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      301 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      361 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      421 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      481 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      541 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      601 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      661 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      721 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      781 cggtcgaccg atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt
      841 ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga
      901 atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt
      961 ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg
     1021 ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat
     1081 tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact
     1141 tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat
     1201 ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacac cctggtcatc
     1261 atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac
     1321 tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctc tttcctacag
     1381 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attccgcggg
     1441 cggccgctct agaggatccg ctagactgac atggcgtgtt caacgctccc aaaatcccct
     1501 aaagataaga ttgacccgcg ggacctccta atccccttaa ttctcttcct gtctctcaaa
     1561 ggggccagat ccgcagcacc cggctccagc cctcaccagg tctacaacat tacctgggaa
     1621 gtgaccaatg gggatcggga gacagtatgg gcaatatcag gcaaccaccc tctgtggact
     1681 tggtggccag tcctcacccc agatttgtgt atgttagctc tcagtgggcc gccccactgg
     1741 gggctagagt atcaggcccc ctattcctcg cccccggggc ccccttgttg ctcagggagc
     1801 agcgggagca gtgcaggctg ttccagagac tgcgacgagc ccttgacctc cctcacccct
     1861 cggtgcaaca ctgcctggaa cagacttaag ctagatcagg taactcataa atcaagtgag
     1921 ggattttatg tctgccccgg gtcacatcgc ccccgggaag ccaagtcctg tggaggtcca
     1981 gactccttct actgtgcctc ttggggctgc gagacaaccg gtagagtata ctggaagccc
     2041 tcctcctctt gggactacat cacagtggac aacaatctca ccactagcca ggctgtccag
     2101 gtatgcaaag acaataagtg gtgcaatccc ttggctatcc agtttacaaa cgccgggaaa
     2161 caggtcacct catggacaac tggacactat tggggtctac gtctttatgt ctctgggcgg
     2221 gacccggggc ttactttcgg gatccgactc agatatcaaa atctaggacc tcgggtcccg
     2281 ataggaccga accccgtcct ggcagaccaa ctttcgctcc cgcgacctaa tcccctaccc
     2341 aaacctgcca agtctccccc cgcctctaat tcgactccca cattgatttc cgctagcatg
     2401 gcggaaggct ccgtcgccag gcagcctgac ctcttgacct gcgaacatga agagatccac
     2461 ctcgccggct cgatccagcc gcatggcgcg cttctggtcg tcagcgaaca tgatcatcgc
     2521 gtcatccagg ccagcgccaa cgccgcggaa tttctgaatc tcggaagcgt actcggcgtt
     2581 ccgctcgccg agatcgacgg cgatctgttg atcaagatcc tgccgcatct cgatcccacc
     2641 gccgaaggca tgccggtcgc ggtgcgctgc cggatcggca atccctctac ggagtactgc
     2701 ggtctgatgc atcggcctcc ggaaggcggg ctgatcatcg aactcgaacg tgccggcccg
     2761 tcgatcgatc tgtcaggcac gctggcgccg gcgctggagc ggatccgcac ggcgggttca
     2821 ctgcgcgcgc tgtgcgatga caccgtgctg ctgtttcagc agtgcaccgg ctacgaccgg
     2881 gtgatggtgt atcgtttcga tgagcaaggc cacggcctgg tattctccga gtgccatgtg
     2941 cctgggctcg aatcctattt cggcaaccgc tatccgtcgt cgactgtccc gcagatggcg
     3001 cggcagctgt acgtgcggca gcgcgtccgc gtgctggtcg acgtcaccta tcagccggtg
     3061 ccgctggagc cgcggctgtc gccgctgacc gggcgcgatc tcgacatgtc gggctgcttc
     3121 ctgcgctcga tgtcgccgtg ccatctgcag ttcctgaagg acatgggcgt gcgcgccacc
     3181 ctggcggtgt cgctggtggt cggcggcaag ctgtggggcc tggttgtctg tcaccattat
     3241 ctgccgcgct tcatccgttt cgagctgcgg gcgatctgca aacggctcgc cgaaaggatc
     3301 gcgacgcgga tcaccgcgct tgagagcgct agcgctgttg ccggcgcccc gtcccccact
     3361 cccactcagc ccccgccagc aggaacggga gacaggttac taaatctagt acagggagct
     3421 taccaggcac tcaaccttac caaccctgat aaaactcaag agtgctggtt atgcctagtg
     3481 tctggacccc cctattacga aggggttgcc gtcctaggta cttattccaa ccatacctct
     3541 gccccagcta actgctccgt ggcctcccaa cacaagttga ccctgtccga agtgactgga
     3601 cggggactct gcataggaac agtcccaaaa actcaccagg ccctgtgcaa cactaccctt
     3661 aagatagaca aagggtctta ctatctagtt gcccccacag gaactacgtg ggcatgtaac
     3721 actggactca ctccatgcct atctgccacc gtgcttaatc gcaccactga ctattgcgtt
     3781 ctcgtagagt tatggcccag ggtcacctac catcctccca gttacgtcta tagccagttt
     3841 gaaaaatcct atagacataa aagagaacca gtgtccttaa ccttggccct attattaggt
     3901 gggctaacta tgggtggcat cgccgcggga gtagggacag gaactaccgc cctggtcgcc
     3961 acccagcagt tccagcagct ccatgctgcc gtacaagatg atctcaaaga agtcgaaaag
     4021 tcaattacta acctagaaaa gtctcttact tcgttgtctg aggttgtgct gcagaatcga
     4081 cgaggcctag acctgttgtt cctaaaagaa ggaggactgt gtgctgccct aaaagaagaa
     4141 tgttgtttct atgctgacca tacaggccta gtaagagata gtatggccaa attaagagag
     4201 agactcactc agagacaaaa actatttgag tcgagccaag gatggttcga aggattgttt
     4261 aacagatccc cctggtttac cacgttaata tccaccatca tggggcctct cattatactc
     4321 ctactaattc tgctttttgg accctgcatt cttaatcgat tagtccaatt tgttaaagac
     4381 aggatatcag tggtccaggc tctagttttg actcaacaat atcaccagct gaagccacta
     4441 gaatacgagc catgactcga gggcccagat ctttttccct ctgccaaaaa ttatggggac
     4501 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     4561 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     4621 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     4681 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     4741 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     4801 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     4861 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     4921 gacggatcgg ccgcaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc
     4981 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     5041 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     5101 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     5161 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     5221 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     5281 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     5341 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     5401 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     5461 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     5521 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     5581 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     5641 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     5701 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     5761 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     5821 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     5881 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     5941 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     6001 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     6061 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6121 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6181 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6241 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6301 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6361 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6421 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6481 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6541 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     6601 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     6661 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     6721 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     6781 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     6841 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     6901 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     6961 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7021 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7081 tccccgaaaa gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     7141 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     7201 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     7261 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     7321 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     7381 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     7441 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     7501 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat
     7561 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     7621 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     7681 tcccagtcac gacgttgtaa aacgacggcc agtgagcgcg cgtaatacga ctcactatag
     7741 ggcgaattg
//
