LOCUS       .                       7530 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        113..492
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        493..696
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          808..1380
                     /label="β-globin intron"
                     /note="intron from rabbit β-globin gene"
     CDS             2398..3108
                     /label="EYFP"
                     /note="enhanced YFP"
     polyA_signal    4316..4371
                     /label="β-globin poly(A) signal"
                     /note="rabbit β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(4729..4745)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4753..4769)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4777..4807)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4822..4843)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5131..5719)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5893..6750)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(6751..6855)
                     /label="AmpR promoter"
     rep_origin      complement(6881..7336)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7478..7494
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        7504..7522
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gagctccacc gcggtggcgg ccgctctaga gagcttggcc cattgcatac gttgtatcca
       61 tatcataata tgtacattta tattggctca tgtccaacat taccgccatg ttgacattga
      121 ttattgacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      181 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      241 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      301 tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      361 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      421 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
      481 gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac
      541 tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa
      601 aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt
      661 aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
      721 tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg atccagcctc
      781 cggtcgaccg atcctgagaa cttcagggtg agtttgggga cccttgattg ttctttcttt
      841 ttcgctattg taaaattcat gttatatgga gggggcaaag ttttcagggt gttgtttaga
      901 atgggaagat gtcccttgta tcaccatgga ccctcatgat aattttgttt ctttcacttt
      961 ctactctgtt gacaaccatt gtctcctctt attttctttt cattttctgt aactttttcg
     1021 ttaaacttta gcttgcattt gtaacgaatt tttaaattca cttttgttta tttgtcagat
     1081 tgtaagtact ttctctaatc actttttttt caaggcaatc agggtatatt atattgtact
     1141 tcagcacagt tttagagaac aattgttata attaaatgat aaggtagaat atttctgcat
     1201 ataaattctg gctggcgtgg aaatattctt attggtagaa acaactacac cctggtcatc
     1261 atcctgcctt tctctttatg gttacaatga tatacactgt ttgagatgag gataaaatac
     1321 tctgagtcca aaccgggccc ctctgctaac catgttcatg ccttcttctc tttcctacag
     1381 ctcctgggca acgtgctggt tgttgtgctg tctcatcatt ttggcaaaga attccgcggg
     1441 cggccgctct agaggatccg ctagactgac atggcgtgtt caacgctccc aaaatcccct
     1501 aaagataaga ttgacccgcg ggacctccta atccccttaa ttctcttcct gtctctcaaa
     1561 ggggccagat ccgcagcacc cggctccagc cctcaccagg tctacaacat tacctgggaa
     1621 gtgaccaatg gggatcggga gacagtatgg gcaatatcag gcaaccaccc tctgtggact
     1681 tggtggccag tcctcacccc agatttgtgt atgttagctc tcagtgggcc gccccactgg
     1741 gggctagagt atcaggcccc ctattcctcg cccccggggc ccccttgttg ctcagggagc
     1801 agcgggagca gtgcaggctg ttccagagac tgcgacgagc ccttgacctc cctcacccct
     1861 cggtgcaaca ctgcctggaa cagacttaag ctagatcagg taactcataa atcaagtgag
     1921 ggattttatg tctgccccgg gtcacatcgc ccccgggaag ccaagtcctg tggaggtcca
     1981 gactccttct actgtgcctc ttggggctgc gagacaaccg gtagagtata ctggaagccc
     2041 tcctcctctt gggactacat cacagtggac aacaatctca ccactagcca ggctgtccag
     2101 gtatgcaaag acaataagtg gtgcaatccc ttggctatcc agtttacaaa cgccgggaaa
     2161 caggtcacct catggacaac tggacactat tggggtctac gtctttatgt ctctgggcgg
     2221 gacccggggc ttactttcgg gatccgactc agatatcaaa atctaggacc tcgggtcccg
     2281 ataggaccga accccgtcct ggcagaccaa ctttcgctcc cgcgacctaa tcccctaccc
     2341 aaacctgcca agtctccccc cgcctctaat tcgactccca cattgatttc cgctagcagc
     2401 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta
     2461 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg
     2521 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc
     2581 accttcggct acggcctgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac
     2641 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac
     2701 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc
     2761 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag
     2821 tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag
     2881 gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac
     2941 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc
     3001 taccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag
     3061 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaaggc tagcgctgtt
     3121 gccggcgccc cgtcccccac tcccactcag cccccgccag caggaacggg agacaggtta
     3181 ctaaatctag tacagggagc ttaccaggca ctcaacctta ccaaccctga taaaactcaa
     3241 gagtgctggt tatgcctagt gtctggaccc ccctattacg aaggggttgc cgtcctaggt
     3301 acttattcca accatacctc tgccccagct aactgctccg tggcctccca acacaagttg
     3361 accctgtccg aagtgactgg acggggactc tgcataggaa cagtcccaaa aactcaccag
     3421 gccctgtgca acactaccct taagatagac aaagggtctt actatctagt tgcccccaca
     3481 ggaactacgt gggcatgtaa cactggactc actccatgcc tatctgccac cgtgcttaat
     3541 cgcaccactg actattgcgt tctcgtagag ttatggccca gggtcaccta ccatcctccc
     3601 agttacgtct atagccagtt tgaaaaatcc tatagacata aaagagaacc agtgtcctta
     3661 accttggccc tattattagg tgggctaact atgggtggca tcgccgcggg agtagggaca
     3721 ggaactaccg ccctggtcgc cacccagcag ttccagcagc tccatgctgc cgtacaagat
     3781 gatctcaaag aagtcgaaaa gtcaattact aacctagaaa agtctcttac ttcgttgtct
     3841 gaggttgtgc tgcagaatcg acgaggccta gacctgttgt tcctaaaaga aggaggactg
     3901 tgtgctgccc taaaagaaga atgttgtttc tatgctgacc atacaggcct agtaagagat
     3961 agtatggcca aattaagaga gagactcact cagagacaaa aactatttga gtcgagccaa
     4021 ggatggttcg aaggattgtt taacagatcc ccctggttta ccacgttaat atccaccatc
     4081 atggggcctc tcattatact cctactaatt ctgctttttg gaccctgcat tcttaatcga
     4141 ttagtccaat ttgttaaaga caggatatca gtggtccagg ctctagtttt gactcaacaa
     4201 tatcaccagc tgaagccact agaatacgag ccatgactcg agggcccaga tctttttccc
     4261 tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc tggctaataa
     4321 aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg
     4381 acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta gagtttggca
     4441 acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg tcatcagtat
     4501 atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac ttgaggttag
     4561 atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa attttcctta
     4621 catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat agctgtccct
     4681 cttctcttat ggagatccct cgacggatcg gccgcaattc gtaatcatgt catagctgtt
     4741 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     4801 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     4861 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     4921 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     4981 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     5041 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     5101 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     5161 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     5221 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     5281 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     5341 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     5401 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     5461 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     5521 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
     5581 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     5641 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     5701 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     5761 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     5821 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     5881 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     5941 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6001 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6061 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6121 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6181 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6241 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     6301 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     6361 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     6421 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     6481 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     6541 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     6601 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     6661 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     6721 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     6781 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     6841 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
     6901 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
     6961 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
     7021 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
     7081 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
     7141 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
     7201 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
     7261 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
     7321 ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga agggcgatcg
     7381 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
     7441 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgagcgc
     7501 gcgtaatacg actcactata gggcgaattg
//
