LOCUS       V012859                 6594 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V012859
VERSION     V012859
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6594)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6594
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1533..1551
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    1564..1671
                     /label="MCS"
                     /note="pBluescript multiple cloning site"
     promoter        complement(1680..1698)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1711..1727)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      2073..2159
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2251..2278
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             complement(2720..3367)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
     CDS             complement(5329..6060)
                     /gene="ermC"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from
                     Staphylococcus aureus. Accession#: P02979"
     rep_origin      6329..6594
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
       61 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
      121 gtgagcattg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
      181 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
      241 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
      301 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
      361 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
      421 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
      481 agtcagtgag cgaggaagcg gaagaagctt actttattgt tttgccattt taaaaggttc
      541 aaataaaata tttttcataa aattatcaga tgttttttaa aaaatgaaat aaaagaagaa
      601 aaaaatagaa aatacactaa cccaaagaag cgcgtaatat cgagggttta agagacaata
      661 agaaaaaaag attgaaaaat gacattaaat tcttgacagg gagagatagg tttgatagaa
      721 tataatagtt gtcgcgagag acgacgttga agcgcgacaa gctagaccat tgaaaactga
      781 ataaagaaga atgactcatg tgatgtagca atacatcaaa aatctgtcaa tcaataatga
      841 aagacaagcc atcaattcgg tgacttaaat actttatttg agagtttgat cctctagagt
      901 cgacctgcag gctttaacgt aggcaaagct cagggtagac tttgaatgga cagaaacatg
      961 acatatctct tgaaaggatg attgtggtgg tgaaaacaga taaaatctcc tcctgaatac
     1021 agtaaatcac attcaggagg agataaaatt gtttaaacaa atagacgaaa attatctgcg
     1081 aaaagagcac tttcaccatt atatgacgtt aacccgatgc tcatatagct tggtgatcaa
     1141 tctagacatc acgaaattgc atgcaatatt aaaagaaaaa aagctgaaag tatatcctgt
     1201 gcaaatttat ttgttagcaa gagctgtgca aaaaattcct gagtttcgga tggatcaagt
     1261 gaacgatgaa cttggttact gggagattct ccatcctagt tatacgattc taaataaaga
     1321 aacaaagacg ttttcaagta tttggacgcc ttttgatgaa aactttgctc agttttataa
     1381 aagctgtgta gccgatattg aaacatttag caaaagcagc aacctatttc cgaaacctca
     1441 tatgccagaa aacatgttca atatttcaag tctaccgtgg attgatttta cttcttttaa
     1501 ccttaatgta tctacagatg aagctagcgc gcaattaacc ctcactaaag ggaacaaaag
     1561 ctggagctcc accgcggtgg cggccgctct agaactagtg gatcccccgg gctgcaggaa
     1621 ttcgatatca agcttatcga taccgtcgac ctcgaggggg ggcccggtac ccaattcgcc
     1681 ctatagtgag tcgtattacg cgcgcaattc actggccgtc gttttacaac gtcgtgactg
     1741 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     1801 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatta
     1861 gagatcaatt ccctgttttg gcggatgaga gaagattttc agcctgatac agattaaatc
     1921 agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg cggtggtccc
     1981 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
     2041 tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct cagtcgaaag
     2101 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt aggacaaatc
     2161 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
     2221 cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat ggcctttttg
     2281 cgtttctaca aactcttcct gtcgtcatat ctacaagcca tccccccaca gatacggtaa
     2341 actagcctcg tttttgcatc aggaaagcag ggaattgatc tgatgggtag aaccttgggc
     2401 tttgcccaaa cccaccaacg gatggccgtt ggattccaac attcaatccc cccaaccccc
     2461 cgcatctccg gggggctccc tcgccggttg gcgtcaaaca aacgagtttg tttgagtgct
     2521 gaaagggttc gggtagcact gagcgagtca actccttaat cctccctacg gtccggttac
     2581 cgttgactca cgccataccc atcgaatggc gaatggcgga tctggagctg taatataaaa
     2641 accttcttca actaacgggg caggttagtg acattagaaa accgactgta aaaagtacag
     2701 tcggcattat ctcatattat aaaagccagt cattaggcct atctgacaat tcctgaatag
     2761 agttcataaa caatcctgca tgataaccat cacaaacaga atgatgtacc tgtaaagata
     2821 gcggtaaata tattgaatta cctttattaa tgaattttcc tgctgtaata atgggtagaa
     2881 ggtaattact attattattg atatttaagt taaacccagt aaatgaagtc catggaataa
     2941 tagaaagaga aaaagcattt tcaggtatag gtgttttggg aaacaatttc cccgaaccat
     3001 tatatttctc tacatcagaa aggtataaat cataaaactc tttgaagtca ttctttacag
     3061 gagtccaaat accagagaat gttttagata caccatcaaa aattgtataa agtggctcta
     3121 acttatccca ataacctaac tctccgtcgc tattgtaacc agttctaaaa gctgtatttg
     3181 agtttatcac ccttgtcact aagaaaataa atgcagggta aaatttatat ccttcttgtt
     3241 ttatgtttcg gtataaaaca ctaatatcaa tttctgtggt tatactaaaa gtcgtttgtt
     3301 ggttcaaata atgattaaat atctcttttc tcttccaatt gtctaaatca attttattaa
     3361 agttcatttg atatgcctcc taaattttta tctaaagtga atttaggagg cttacttgtc
     3421 tgctttcttc attagaatca atcctttttt aaaagtcaat attactgtaa cataaatata
     3481 tattttaaaa atatcccact ttatccaatt ttcgtttgtt gaactaatgg gtgctttagt
     3541 tgaagaataa agaccacatt aaaaaatgtg gtcttttgtg tttttttaaa ggatttgagc
     3601 gtagcgaaaa atccttttct ttcttatctt gataataagg gtaactattg ccgcgcctga
     3661 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataagc cggtcttttt
     3721 cgattttggc gaagccgaaa tcgggtcttt tcttatcttg atactatata gaaacaacat
     3781 catttttcaa aattaggtca aagccttgtg tatcaagggt ttgatggttc tttgacaggt
     3841 aaaaactcct tctgctatta ttaaggtgtc gaatcaaaat aatagaatgc tagagaacta
     3901 gctcagaagg agtttttttg ttgatttatt catctgaaaa tgattatagc atcctcgaag
     3961 ataaaaccgc aacaggtaaa aagcgggatt ggaaggggaa aaagagacgg acgaacctca
     4021 tggcggagca ttacgaagcg ttagagagta agattggggc accttactat ggcaaaaagg
     4081 ctgaaaaact aattagttgt gcagagtatc tttcgtttaa gagagacccg gagacgggca
     4141 agttaaaact gtatcaagcc catttttgta aagtgaggtt atgtccgatg tgtgcgtggc
     4201 gcaggtcgtt aaaaattgct tatcacaata agttgatcgt agaggaagcc aatagacagt
     4261 acggctgcgg atggattttt ctcacgctga cgattcgaaa tgtaaaggga gaacggctga
     4321 agccacaaat ttctgcgatg atggaaggct ttaggaaact gttccagtac aaaaaagtaa
     4381 aaacttcggt tcttggattt ttcagagctt tagagattac caaaaatcat gaagaagata
     4441 catatcatcc tcattttcat gtgttgatac cagtaaggaa aaattatttt gggaaaaact
     4501 atattaagca ggcggagtgg acgagccttt ggaaaaaggc gatgaaattg gattacactc
     4561 caattgtcga tattcgtcga gtgaaaggta aagctaagat tgacgctgaa cagattgaaa
     4621 acgatgtgcg gaacgcaatg atggagcaaa aagctgttct cgaaatctct aaatatccgg
     4681 ttaaggatac ggatgttgtg cgcggtaata aggtgactga agacaatctg aacacggtgc
     4741 tttacttgga tgatgcgttg gcagctcgaa ggttaattgg atacggtggc attttgaagg
     4801 agatacataa agagctgaat cttggtgatg cggaggacgg cgatctggtc aagattgagg
     4861 aagaagatga cgaggttgca aatggtgcat ttgaggttat ggcttattgg catcctggca
     4921 ttaaaaatta cataatcaaa taaaaaaagc agacctttag aaggcctgct tttttaacta
     4981 acccatttgt attgtgttga aatatgtttt gtatggtgca ctctcagtac aatctgctct
     5041 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     5101 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     5161 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     5221 ctatttttat aggttaatgt catgataata atggtttctt agcgattcac aaaaaatagg
     5281 cacacgaaaa acaagttaag ggatgcagtt tatgcatccc ttaacttact tattaaataa
     5341 tttatagcta ttgaaaagag ataagaattg ttcaaagcta atattgttta aatcgtcaat
     5401 tcctgcatgt tttaaggaat tgttaaattg attttttgta aatattttct tgtattcttt
     5461 gttaacccat ttcataacga aataattata cttttgttta tctttgtgtg atattcttga
     5521 tttttttcta cttaatctga taagtgagct attcacttta ggtttaggat gaaaatattc
     5581 tcttggaacc atacttaata tagaaatatc aacttctgcc attaaaagta atgccaatga
     5641 gcgttttgta tttaataatc ttttagcaaa cccgtattcc acgattaaat aaatctcatt
     5701 agctatacta tcaaaaacaa ttttgcgtat tatatccgta cttatgttat aaggtatatt
     5761 accatatatt ttataggatt ggtttttagg aaatttaaac tgcaatatat ccttgtttaa
     5821 aacttggaaa ttatcgtgat caacaagttt attttctgta gttttgcata atttatggtc
     5881 tatttcaatg gcagttacga aattacacct ctttactaat tcaagggtaa aatggccttt
     5941 tcctgagccg atttcaaaga tattatcatg ttcatttaat cttatatttg tcattatttt
     6001 atctatatta tgttttgaag taataaagtt ttgactgtgt tttatatttt tctcgttcat
     6061 tataaccctc tttaatttgg ttatatgaat tttgcttatt aacgattcat tataaccact
     6121 tattttttgt ttggttgata atgaactgtg ctgattacaa aaatactaaa aatgcccata
     6181 ttttttcctc cttataaaat tagtataatt atagcacgaa aaggatctag gtgaagatcc
     6241 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6301 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6361 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6421 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     6481 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6541 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt ctta
//
