LOCUS       40924_22636        6382 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6382)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    542..564
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     promoter        626..971
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             982..1914
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
     polyA_signal    1974..2150
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     primer_bind     2314..2330
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2337..2355
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(2364..2469)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     CDS             2479..4128
                     /label=luciferase
                     /note="firefly luciferase"
     polyA_signal    4188..4364
                     /label=CaMV poly(A) signal
                     /note="CaMV poly(A) signal"
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    4374..4398
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(4489..5077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5251..6063)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      6354..6382
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgccag cgcctttgcc
      421 cacaacccgg cggccggccg caacagatcg ttttataaat tttttttttt gaaaaagaaa
      481 aagcccgaaa ggcggcaacc tctcgggctt ctggatttcc gatccccgga attagagatc
      541 ttggcaggat atattgtggt gtaacgttat cgtaccccta ctccaaaaat gtcaaagata
      601 cagtctcaga agaccaaagg gctattgaga cttttcaaca aagggtaatt tcgggaaacc
      661 tcctcggatt ccattgccca gctatctgtc acttcatcga aaggacagta gaaaaggaag
      721 gtggctccta caaatgccat cattgcgata aaggaaaggc tatcattcaa gatgcctctg
      781 ccgacagtgg tcccaaagat ggacccccac ccacgaggag catcgtggaa aaagaagacg
      841 ttccaaccac gtcttcaaag caagtggatt gatgtgacat ctccactgac gtaagggatg
      901 acgcacaatc ccactatcct tcgcaagacc cttcctctat ataaggaagt tcatttcatt
      961 tggagaggac agcccaccac catgacttcg aaagtttatg atccagaaca aaggaaacgg
     1021 atgataactg gtccgcagtg gtgggccaga tgtaaacaaa tgaatgttct tgattcattt
     1081 attaattatt atgattcaga aaaacatgca gaaaatgctg ttattttttt acatggtaac
     1141 gcggcctctt cttatttatg gcgacatgtt gtgccacata ttgagccagt agcgcggtgt
     1201 attataccag accttattgg tatgggcaaa tcaggcaaat ctggtaatgg ttcttatagg
     1261 ttacttgatc attacaaata tcttactgca tggtttgaac ttcttaattt accaaagaag
     1321 atcatttttg tcggccatga ttggggtgct tgtttggcat ttcattatag ctatgagcat
     1381 caagataaga tcaaagcaat agttcacgct gaaagtgtag tagatgtgat tgaatcatgg
     1441 gatgaatggc ctgatattga agaagatatt gcgttgatca aatctgaaga aggagaaaaa
     1501 atggttttgg agaataactt cttcgtggaa accatgttgc catcaaaaat catgagaaag
     1561 ttagaaccag aagaatttgc agcatatctt gaaccattca aagagaaagg tgaagttcgt
     1621 cgtccaacat tatcatggcc tcgtgaaatc ccgttagtaa aaggtggtaa acctgacgtt
     1681 gtacaaattg ttaggaatta taatgcttat ctacgtgcaa gtgatgattt accaaaaatg
     1741 tttattgaat cggacccagg attcttttcc aatgctattg ttgaaggtgc caagaagttt
     1801 cctaatactg aatttgtcaa agtaaaaggt cttcattttt cgcaagaaga tgcacctgat
     1861 gaaatgggaa aatatatcaa atcgttcgtt gagcgagttc tcaaaaatga acaataattc
     1921 tagccggtac gctgaaatca ccagtctctc tctacaaatc tatctctctc tattttctcc
     1981 ataaataatg tgtgagtagt ttcccgataa gggaaattag ggttcttata gggtttcgct
     2041 catgtgttga gcatataaga aacccttagt atgtatttgt atttgtaaaa tacttctatc
     2101 aataaaattt ctaattccta aaaccaaaat ccagtactaa aatccagatc gataacatta
     2161 acgtttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     2221 gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     2281 gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     2341 acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac ggtatcgata
     2401 agcttgatat cgaattcctg cagcccgggg gatccactag ttctagagcg gccgccaccg
     2461 cggtggagat cgaattccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc
     2521 tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
     2581 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct
     2641 gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg gctgaataca
     2701 aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc ggtgttgggc
     2761 gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga acgtgaattg
     2821 ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa ggggttgcaa
     2881 aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat catggattct
     2941 aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca tctacctccc
     3001 ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac aattgcactg
     3061 atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc tcatagaact
     3121 gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat cattccggat
     3181 actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac tacactcgga
     3241 tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga gctgtttctg
     3301 aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct attctccttc
     3361 ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga aattgcttct
     3421 ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt ccatctgcca
     3481 ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat tacacccgag
     3541 ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc gaaggttgtg
     3601 gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg tgtgagaggt
     3661 cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt gattgacaag
     3721 gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca cttcttcatc
     3781 gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc cgctgaattg
     3841 gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg tcttcccgac
     3901 gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa gacgatgacg
     3961 gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa gttgcgcgga
     4021 ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga cgcaagaaaa
     4081 atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta attctagaga
     4141 attcgctgaa atcaccagtc tctctctaca aatctatctc tctctatttt ctccataaat
     4201 aatgtgtgag tagtttcccg ataagggaaa ttagggttct tatagggttt cgctcatgtg
     4261 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     4321 atttctaatt cctaaaacca aaatccagta ctaaaatcca gatccactag ccttgacagg
     4381 atatattggc gggtaaacta agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa
     4441 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     4501 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     4561 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     4621 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     4681 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     4741 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     4801 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4861 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4921 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaagaag
     4981 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     5041 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     5101 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     5161 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     5221 tatatatgtg taacattggt ctagtgatta gaaaaactca tcgagcatca aatgaaactg
     5281 caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt tctgtaatga
     5341 aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc ggtctgcgat
     5401 tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa taaggttatc
     5461 aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa gtttatgcat
     5521 ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat cactcgcatc
     5581 aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt
     5641 aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg ccagcgcatc
     5701 aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg ttttccctgg
     5761 gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct tgatggtcgg
     5821 aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa caacattggc
     5881 aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc catacaatcg
     5941 gtagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc catataaatc
     6001 agcatccatg ttggaattta atcgcggcct tgagcaagac gtttcccgtt gaatatggct
     6061 cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc atgatgatat
     6121 atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc tttgttgaat
     6181 aaatcgaact tttgctgagt tgaaggatca gatcacgcat cttcccgaca acgcagaccg
     6241 ttccgtggca aagcaaaagt tcaaaatcac caactggtcc acctacaaca aagctctcat
     6301 caaccgtggc tccctcactt tctggctgga tgatggggcg attcaggcga tccccatcca
     6361 acagcccgcc gtcgagcggg ct
//
