LOCUS       40924_22598        4448 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Agrobacterium binary vector with kanamycin-and 
            bialophos/phosphinothricin-resistance genes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM.
  TITLE     pGreen: a versatile and flexible binary Ti vector for 
            Agrobacterium-mediated plant transformation.
  JOURNAL   Plant Mol. Biol. 2000;42:819-32.
  PUBMED    10890530
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   pGreen website
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4448)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2000"; volume: "42"; pages: "819-32"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     This plasmid must be transformed into an Agrobacterium strain that 
            also carries a vector such as pSoup.
FEATURES             Location/Qualifiers
     source          1..4448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      63..498
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    633..655
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     terminator      complement(679..930)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(953..1501)
                     /codon_start=1
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
                     /translation="MSPERRPADIRRATEADMPAVCTIVNHYIQTSTVNFRTEPQEPQE
                     WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
                     TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
                     WQLDFSLPVPPRPVLPVTEI"
     promoter        complement(1544..1721)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     1968..1984
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1991..2009
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(2018..2125)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(2138..2156)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2177..2193)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2201..2217)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2225..2256)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2271..2292)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    2531..2555
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(2646..3234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3408..4220)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
ORIGIN
        1 agctctcatc aaccgtggct ccctcacttt ctggctggat gatggggcga ttcaggcgat
       61 ccccatccaa cagcccgccg tcgagcgggc ttttttatcc ccggaagcct gtggatagag
      121 ggtagttatc cacgtgaaac cgctaatgcc ccgcaaagcc ttgattcacg gggctttccg
      181 gcccgctcca aaaactatcc acgtgaaatc gctaatcagg gtacgtgaaa tcgctaatcg
      241 gagtacgtga aatcgctaat aaggtcacgt gaaatcgcta atcaaaaagg cacgtgagaa
      301 cgctaatagc cctttcagat caacagcttg caaacacccc tcgctccggc aagtagttac
      361 agcaagtagt atgttcaatt agcttttcaa ttatgaatat atatatcaat tattggtcgc
      421 ccttggcttg tggacaatgc gctacgcgca ccggctccgc ccgtggacaa ccgcaagcgg
      481 ttgcccaccg tcgagcgcca gcgcctttgc ccacaacccg gcggccggcc gcaacagatc
      541 gttttataaa tttttttttt tgaaaaagaa aaagcccgaa aggcggcaac ctctcgggct
      601 tctggatttc cgatccccgg aattagagat cttggcagga tatattgtgg tgtaacgtta
      661 tcagcttgca tgccggtcga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
      721 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
      781 tcaaaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
      841 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa
      901 gaaactttat tgccaaatgt ttgaacgatc tgcttgactc taggggtcat cagatttcgg
      961 tgacgggcag gaccggacgg ggcggcaccg gcaggctgaa gtccagctgc cagaaaccca
     1021 cgtcatgcca gttcccgtgc ttgaagccgg ccgcccgcag catgccacgg ggggcatatc
     1081 cgagcgcctc gtgcatgcgc acgctcgggt cgttgggcag cccgatgaca gcgaccacgc
     1141 tcttgaagcc ctgtgcctcc agggacttca gcaggtgggt gtagagcgtg gagcccagtc
     1201 ccgtccgctg gtggcggggg gagacgtaca cggttgactc ggccgtccag tcgtaggcgt
     1261 tgcgtgcctt ccagggaccc gcgtaggcga tgccggcgac ctcgccgtcc acctcggcga
     1321 cgagccaggg atagcgctcc cgcagacgga cgaggtcgtc cgtccactcc tgcggttcct
     1381 gcggctcggt acggaagttg accgtgcttg tctggatgta gtggttgacg atggtgcaga
     1441 ccgccggcat gtccgcctcg gtggcacggc ggatgtcggc cgggcgtcgt tctgggctca
     1501 tggtagatcc ccctcgatcg agttgagagt gaatatgaga ctctaattgg ataccgaggg
     1561 gaatttatgg aacgtcagtg gagcattttt gacaagaaat atttgctagc tgatagtgac
     1621 cttaggcgac ttttgaacgc gcaataatgg tttctgacgt atgtgcttag ctcattaaac
     1681 tccagaaacc cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa
     1741 acggcttgtc ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctcatgtatc
     1801 gataacatta acgtttacaa tttcgcgcca ttcgccattc aggctgcgca actgttggga
     1861 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     1921 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     1981 cagtgaattg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt
     2041 cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag
     2101 agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattccga
     2161 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     2221 cacacaacat acgagccgga aghcataaag tgtaaagcct ggggtgccta atgagtgagc
     2281 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     2341 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     2401 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     2461 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     2521 atgaaggcct tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga
     2581 gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2641 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2701 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2761 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2821 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2881 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2941 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3001 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3061 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3121 gttaccttcg gaagaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3181 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3241 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3301 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3361 tttaaatcaa tctaaagtat atatgtgtaa cattggtcta gtgattagaa aaactcatcg
     3421 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     3481 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     3541 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     3601 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     3661 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     3721 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     3781 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     3841 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     3901 aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     3961 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     4021 tctgtaacaa cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     4081 ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc gcgagcccat
     4141 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga gcaagacgtt
     4201 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     4261 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     4321 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt
     4381 cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc
     4441 tacaacaa
//
