LOCUS       40924_22578        4632 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Compact Agrobacterium binary vector with a kanamycin-resistance 
            genes for bacterial and plant transformation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Hellens RP, Edwards EA, Leyland NR, Bean S, Mullineaux PM.
  TITLE     pGreen: a versatile and flexible binary Ti vector for 
            Agrobacterium-mediated plant transformation.
  JOURNAL   Plant Mol. Biol. 2000;42:819-32.
  PUBMED    10890530
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   pGreen website
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 4632)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol. 
            Biol."; date: "2000"; volume: "42"; pages: "819-32"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     This plasmid must be transformed into an Agrobacterium strain that 
            also carries a vector such as pSoup.
FEATURES             Location/Qualifiers
     source          1..4632
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(555..734)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     primer_bind     981..997
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1004..1022
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    complement(1031..1138)
                     /label=MCS
                     /note="pBluescript multiple cloning site"
     promoter        complement(1151..1169)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1190..1206)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1214..1230)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1238..1269)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1284..1305)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    1544..1568
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(1659..2247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2351..3163)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYGLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3454..3889
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
     misc_feature    4022..4044
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     terminator      complement(4068..4319)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(join(4362..4632,1..521))
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERGRTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTQGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
ORIGIN
        1 agcctggcga acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga
       61 tcgacaagac cggcttccat ccgagtacgt cctcgctcga tgcgatgttt cgcttggtgg
      121 tcgaatgggc aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg
      181 gatactttct cggcaggagc aaggtgagat gacaggagat cctgccccgg cacttcgccc
      241 aatagcagcc agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg
      301 cccgtcgtgg ccagccacga tagccgcgct gcctcgtctt ggagttcatt cagggcaccg
      361 gacaggtcgg tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg
      421 gcatcagagc agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa
      481 gcggccggag aacctgcgtg caatccatct tgttcaatca tgcctcgatc gagttgagag
      541 tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt
      601 tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg
      661 gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga gtggctcctt
      721 caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg
      781 gtcataacgt gactccctta attctcatgt atcgataaca ttaacgttta caatttcgcg
      841 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
      901 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
      961 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
     1021 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
     1081 attcctgcag cccgggggat ccactagttc tagagcggcc gccaccgcgg tggagctcca
     1141 gcttttgttc cctttagtga gggttaattc cgagcttggc gtaatcatgg tcatagctgt
     1201 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaaghcata
     1261 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     1321 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     1381 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     1441 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1501 tccacagaat caggggataa cgcaggaaag aacatgaagg ccttgacagg atatattggc
     1561 gggtaaacta agtcgctgta tgtgtttgtt tgagatctca tgtgagcaaa aggccagcaa
     1621 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     1681 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     1741 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     1801 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     1861 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     1921 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     1981 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     2041 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     2101 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     2161 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     2221 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     2281 gctcagtgga acgaaaactc acgttaaggg attttggtca tggttacaac caattaacca
     2341 attctgatta gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat
     2401 tatcaatacc atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc
     2461 agttccatag gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa
     2521 tacaacctat taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag
     2581 tgacgactga atccggtgag aatggcaaaa gtttatgcat ttctttccag acttgttcaa
     2641 caggccagcc attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc
     2701 gtgattgcgc ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag
     2761 gaatcgaatg caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat
     2821 caggatattc ttctaatacc tggaatgctg ttttccctgg gatcgcagtg gtgagtaacc
     2881 atgcatcatc aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca
     2941 gccagtttag tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt
     3001 tcagaaacaa ctctggcgca tcgggcttcc catacaatcc atagattgtc gcacctgatt
     3061 gcccgacatt atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta
     3121 atcgcggcct ggagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
     3181 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
     3241 aacatcagag attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt
     3301 tgaaggatca gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt
     3361 tcaaaatcac caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt
     3421 tctggctgga tgatggggcg attcaggcga tccccatcca acagcccgcc gtcgagcggg
     3481 cttttttatc cccggaagcc tgtggataga gggtagttat ccacgtgaaa ccgctaatgc
     3541 cccgcaaagc cttgattcac ggggctttcc ggcccgctcc aaaaactatc cacgtgaaat
     3601 cgctaatcag ggtacgtgaa atcgctaatc ggagtacgtg aaatcgctaa taaggtcacg
     3661 tgaaatcgct aatcaaaaag gcacgtgaga acgctaatag ccctttcaga tcaacagctt
     3721 gcaaacaccc ctcgctccgg caagtagtta cagcaagtag tatgttcaat tagcttttca
     3781 attatgaata tatatatcaa ttattggtcg cccttggctt gtggacaatg cgctacgcgc
     3841 accggctccg cccgtggaca accgcaagcg gttgcccacc gtcgagcgcc agcgcctttg
     3901 cccacaaccc ggcggccggc cgcaacagat cgttttataa attttttttt ttgaaaaaga
     3961 aaaagcccga aaggcggcaa cctctcgggc ttctggattt ccgatccccg gaattagatc
     4021 ttggcaggat atattgtggt gtaacgttat cagcttgcat gccggtcgat ctagtaacat
     4081 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     4141 gtattaaatg tataattgcg ggactctaat caaaaaaccc atctcataaa taacgtcatg
     4201 cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
     4261 gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatct
     4321 gcttgactct agctagagtc cgaaccccag agtcccgctc agaagaactc gtcaagaagg
     4381 cgatagaagg cgatgcgctg cgaatcggga gcggcgatac cgtaaagcac gaggaagcgg
     4441 tcagcccatt cgccgccaag ctcttcagca atatcacggg tagccaacgc tatgtcctga
     4501 tagcggtccg ccacacccag ccggccacag tcgatgaatc cagaaaagcg gccattttcc
     4561 accatgatat tcggcaagca ggcatcgccc tgggtcacga cgagatcctc gccgtcgggc
     4621 atccgcgcct tg
//
