LOCUS       .                       2550 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        87..121
                     /label="J23119(SpeI) promoter"
                     /note="bacterial promoter (Registry of Standard Biological
                     Parts BBa_J23119) modified to end with an SpeI site"
     primer_bind     495..511
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    complement(515..571)
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(580..596)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(604..620)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(628..658)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(673..694)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(981..1569)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(1774..2304)
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        complement(2493..2521)
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca tgacattaac
       61 ctataaaaat aggcgtatca cgaggcttga cagctagctc agtcctaggt ataatactag
      121 ttttttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
      181 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      241 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
      301 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc
      361 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      421 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      481 ccagtcacga cgttgtaaaa cgacggccag tgccaagctt gcatgcctgc aggtcgactc
      541 tagaggatcc ccgggtaccg agctcgaatt cgtaatcatg tcatagctgt ttcctgtgtg
      601 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
      661 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
      721 ccagtcggga aacctgtcag ttttagagct agaaatagca agttaaaata aggctagtcc
      781 gttatcaact tgaaaaagtg gcacccttcc tcgctcactg actcgctgcg ctcggtcgtt
      841 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
      901 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
      961 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     1021 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     1081 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     1141 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     1201 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     1261 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     1321 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     1381 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     1441 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     1501 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     1561 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     1621 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     1681 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagc
     1741 cgggaagccg atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa
     1801 gtgcatcact tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc
     1861 gtaatctgct tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgaggag
     1921 attgatgagc gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata
     1981 gatatagatc tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc
     2041 aacaaccgct tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt
     2101 cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg
     2161 accgaaaaga tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt
     2221 gcgaatgatg cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt
     2281 aacatcgttg ctgctgcgta acatcgttgc tgctccataa catcaaacat cgacccacgg
     2341 cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg tacaaaaaaa cagtcataac
     2401 aagccatgaa aaccgccact gcgccgttac caccgctgcg ttcggtcaag gttctggacc
     2461 agttgcgtga gcgcatacgc tacttgcatt acagtttacg aaccgaacag gcttatgtca
     2521 actgggttcg tgccttcatc cgtttccacg
//
