LOCUS       .                       5511 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        183..211
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /label="GST"
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             918..935
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     CDS             1482..1499
                     /label="6xHis"
                     /note="6xHis affinity tag"
     promoter        1814..1918
                     /label="AmpR promoter"
     CDS             1919..2776
                     /label="AmpR"
                     /note="β-lactamase"
     rep_origin      2950..3538
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        3782..3859
                     /label="lacIq promoter"
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3860..4939
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    4955..4976
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4991..5021
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     CDS             5065..5238
                     /label="lacZα"
                     /note="LacZα fragment of β-galactosidase"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg gttccgcgtg gatccggacc taacactgag tttgctctgt
      961 ctctgctgcg taaaaacatc atgactatca ccactagtaa aggcgagttc actggtctgg
     1021 gtattcacga tcgtgtttgt gttattccta ctcatgctca gccgggtgac gatgttctgg
     1081 taaacggtca aaaaattcgt gttaaggata aatacaaact ggttgacccg gaaaacatca
     1141 atctagaact gaccgtactg actctggatc gtaatgaaaa gttccgtgac atccgtggtt
     1201 ttatttctga agacctggaa ggtgtcgacg caaccctggt tgtacatagc aataacttta
     1261 ctaacactat tctggaggtt ggtccggtaa ctatggctgg tctgatcaac ctgtctagca
     1321 ctccgaccaa ccgcatgatt cgttacgact acgcaactaa aactggtcag tgtggtggtg
     1381 ttctgtgcgc aaccggtaag atctttggca tccatgtagg cggtaacggt cgtcagggtt
     1441 tctctgcaca actgaagaag caatactttg tagagaagca gcatcaccat catcaccatt
     1501 gagaattcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa
     1561 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag
     1621 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac
     1681 ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg
     1741 cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt
     1801 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     1861 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     1921 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     1981 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     2041 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     2101 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     2161 tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     2221 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     2281 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     2341 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     2401 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     2461 tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     2521 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     2581 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     2641 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     2701 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     2761 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     2821 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     2881 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     2941 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     3001 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     3061 aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     3121 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     3181 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     3241 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     3301 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     3361 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     3421 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     3481 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     3541 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     3601 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     3661 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     3721 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcataaattc
     3781 cgacaccatc gaatggtgca aaacctttcg cggtatggca tgatagcgcc cggaagagag
     3841 tcaattcagg gtggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg
     3901 tgtctcttat cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac
     3961 gcgggaaaaa gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca
     4021 acaactggcg ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca
     4081 cgcgccgtcg caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt
     4141 ggtggtgtcg atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct
     4201 tctcgcgcaa cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat
     4261 tgctgtggaa gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac
     4321 acccatcaac agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct
     4381 ggtcgcattg ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc
     4441 gcgtctgcgt ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc
     4501 ggaacgggaa ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa
     4561 tgagggcatc gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat
     4621 gcgcgccatt accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga
     4681 cgataccgaa gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg
     4741 cctgctgggg caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa
     4801 gggcaatcag ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac
     4861 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4921 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     4981 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     5041 aacaatttca cacaggaaac agctatgacc atgattacgg attcactggc cgtcgtttta
     5101 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     5161 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     5221 cgcagcctga atggcgaatg gcgctttgcc tggtttccgg caccagaagc ggtgccggaa
     5281 agctggctgg agtgcgatct tcctgaggcc gatactgtcg tcgtcccctc aaactggcag
     5341 atgcacggtt acgatgcgcc catctacacc aacgtaacct atcccattac ggtcaatccg
     5401 ccgtttgttc ccacggagaa tccgacgggt tgttactcgc tcacatttaa tgttgatgaa
     5461 agctggctac aggaaggcca gacgcgaatt atttttgatg gcgttggaat t
//
