LOCUS       pGEX-6P-2.        4985 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing GST fusion proteins with a 
            PreScission protease site. For other reading frames, use pGEX-6P-1 
            or pGEX-6P-3.
ACCESSION   .
VERSION     .
KEYWORDS    pGEX-6P-2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   GE Healthcare
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4985
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             918..941
                     /label=HRV 3C site
                     /note="recognition and cleavage site for human rhinovirus
                     3C and PreScission proteases"
     misc_feature    940..982
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    987..997
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     promoter        1288..1392
                     /label=AmpR promoter
     CDS             1393..2250
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2424..3012
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3256..3333
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3334..4413
                     /label=lacI
                     /note="lac repressor"
     protein_bind    4429..4450
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4465..4495
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             4539..4712
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"
ORIGIN
        1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
       61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
      121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
      181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
      241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
      301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
      361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
      421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
      481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
      541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
      601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
      661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
      721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
      781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
      841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
      901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc ccaggaattc
      961 ccgggtcgac tcgagcggcc gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt
     1021 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     1081 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     1141 ggcgcagcca tgacccagtc acgtagcgat agcggagtgt ataattcttg aagacgaaag
     1201 ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg
     1261 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     1321 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     1381 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     1441 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     1501 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     1561 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     1621 gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     1681 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     1741 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     1801 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     1861 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     1921 gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     1981 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     2041 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     2101 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     2161 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     2221 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     2281 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     2341 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     2401 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     2461 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     2521 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     2581 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     2641 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     2701 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     2761 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     2821 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     2881 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     2941 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     3001 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     3061 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     3121 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     3181 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     3241 caccgcataa attccgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag
     3301 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc
     3361 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac
     3421 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc
     3481 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc
     3541 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa
     3601 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg
     3661 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat
     3721 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat
     3781 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg
     3841 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta
     3901 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa
     3961 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc
     4021 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg
     4081 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg
     4141 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtc aaccaccatc
     4201 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag
     4261 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc
     4321 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     4381 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     4441 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     4501 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acggattcac
     4561 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4621 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4681 cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag
     4741 aagcggtgcc ggaaagctgg ctggagtgcg atcttcctga ggccgatact gtcgtcgtcc
     4801 cctcaaactg gcagatgcac ggttacgatg cgcccatcta caccaacgta acctatccca
     4861 ttacggtcaa tccgccgttt gttcccacgg agaatccgac gggttgttac tcgctcacat
     4921 ttaatgttga tgaaagctgg ctacaggaag gccagacgcg aattattttt gatggcgttg
     4981 gaatt
//
