LOCUS       pGAPZ-A.        3147 bp DNA     circular SYN 13-SEP-2021
DEFINITION  Pichia pastoris vector for constitutive expression of a secreted 
            protein. For other reading frames, use pGAPZ-alpha B or pGAPZ-alpha 
            C.
ACCESSION   .
VERSION     .
KEYWORDS    pGAPZ-A
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3147)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 3147)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Linearize within the GAP promoter for integration.
FEATURES             Location/Qualifiers
     source          1..3147
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..483
                     /label=GAP promoter
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     CDS             493..759
                     /label=alpha-factor secretion signal
                     /note="N-terminal secretion signal from S. cerevisiae 
                     alpha-factor"
     misc_feature    760..828
                     /label=MCS
                     /note="MCS"
                     /note="MCS;multiple cloning site"
     CDS             827..856
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     CDS             872..889
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      968..1214
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
     promoter        1229..1640
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        1647..1694
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1713..2084
                     /label=BleoR
                     /note="antibiotic-binding protein"
     terminator      2153..2400
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     rep_origin      complement(2475..3063)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatcttttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca tctctgaaat
       61 atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc cggggtaaaa
      121 cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct tccaccgccc
      181 gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc cccttgcagc
      241 aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg acctagcagc
      301 ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg catgtcatga
      361 gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca attttggttt
      421 ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca attgaacaac
      481 tatttcgaaa cgatgagatt tccttcaatt tttactgctg ttttattcgc agcatcctcc
      541 gcattagctg ctccagtcaa cactacaaca gaagatgaaa cggcacaaat tccggctgaa
      601 gctgtcatcg gttactcaga tttagaaggg gatttcgatg ttgctgtttt gccattttcc
      661 aacagcacaa ataacgggtt attgtttata aatactacta ttgccagcat tgctgctaaa
      721 gaagaagggg tatctctcga gaaaagagag gctgaagctg aattcacgtg gcccagccgg
      781 ccgtctcgga tcggtacctc gagccgcggc ggccgccagc tttctagaac aaaaactcat
      841 ctcagaagag gatctgaata gcgccgtcga ccatcatcat catcatcatt gagttttagc
      901 cttagacatg actgttcctc agttcaagtt gggcacttac gagaagaccg gtcttgctag
      961 attctaatca agaggatgtc agaatgccat ttgcctgaga gatgcaggct tcatttttga
     1021 tactttttta tttgtaacct atatagtata ggattttttt tgtcattttg tttcttctcg
     1081 tacgagcttg ctcctgatca gcctatctcg cagctgatga atatcttgtg gtaggggttt
     1141 gggaaaatca ttcgagtttg atgtttttct tggtatttcc cactcctctt cagagtacag
     1201 aagattaagt gagaccttcg tttgtgcgga tcccccacac accatagctt caaaatgttt
     1261 ctactccttt tttactcttc cagattttct cggactccgc gcatcgccgt accacttcaa
     1321 aacacccaag cacagcatac taaattttcc ctctttcttc ctctagggtg tcgttaatta
     1381 cccgtactaa aggtttggaa aagaaaaaag agaccgcctc gtttcttttt cttcgtcgaa
     1441 aaaggcaata aaaattttta tcacgtttct ttttcttgaa attttttttt ttagtttttt
     1501 tctctttcag tgacctccat tgatatttaa gttaataaac ggtcttcaat ttctcaagtt
     1561 tcagtttcat ttttcttgtt ctattacaac tttttttact tcttgttcat tagaaagaaa
     1621 gcatagcaat ctaatctaag ggcggtgttg acaattaatc atcggcatag tatatcggca
     1681 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
     1741 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
     1801 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
     1861 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
     1921 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
     1981 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
     2041 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtccgacggc
     2101 ggcccacggg tcccaggcct cggagatccg tccccctttt cctttgtcga tatcatgtaa
     2161 ttagttatgt cacgcttaca ttcacgccct ccccccacat ccgctctaac cgaaaaggaa
     2221 ggagttagac aacctgaagt ctaggtccct atttattttt ttatagttat gttagtatta
     2281 agaacgttat ttatatttca aatttttctt ttttttctgt acagacgcgt gtacgcatgt
     2341 aacattatac tgaaaacctt gcttgagaag gttttgggac gctcgaaggc tttaatttgc
     2401 aagctggaga ccaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2461 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2521 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2581 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2641 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     2701 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2761 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2821 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2881 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     2941 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3001 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3061 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3121 taagggattt tggtcatgca tgagatc
//
