LOCUS       .                       4308 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     promoter        7..483
                     /label="GAP promoter"
                     /note="promoter for Pichia pastoris
                     glyceraldehyde-3-phosphate dehydrogenase"
     terminator      2129..2375
                     /label="AOX1 terminator"
                     /note="transcription terminator for AOX1"
     promoter        2390..2801
                     /label="TEF1 promoter"
                     /note="promoter for EF-1α"
     promoter        2808..2855
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             2874..3245
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     terminator      3314..3561
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     rep_origin      complement(3636..4224)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 agatcttttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca tctctgaaat
       61 atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc cggggtaaaa
      121 cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct tccaccgccc
      181 gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc cccttgcagc
      241 aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg acctagcagc
      301 ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg catgtcatga
      361 gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca attttggttt
      421 ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca attgaacaac
      481 tatttcgaaa cgatgcataa tgcgtcaata ataaattgta caactcaaga ggctttgaac
      541 tcgctggcag cgagcttcaa agacaccgaa ctctctatta ccgaacgcgc ccttgatgag
      601 ttaaactcca agccaaagat tcccgagatc acagcatgga actggacgaa acgatggcag
      661 gaaatctgaa aataacaaat ctcaacacaa catatacaaa acaaacgaat ctcaagcaat
      721 caagcattct acttctattg cagcaattta aatcatttct tttaaagcaa aagcaatttt
      781 ctgaaaattt tcaccattta cgaacgatag cagaaacgat ggatactata atcccaatta
      841 aacacctaga tgccgacggc tcgtccatac tcaagaaatc acctaagata aactaccgcc
      901 aactagcatg tagaattatt ggtgaaattc ccgctgaaaa gattttggat gatgatgagc
      961 ttgcattata taatgaggag ataggcatac attttagccc cgaaatcatc aatgcaaaca
     1021 agctggtcgt ggtcgtaaag gcgactcgtc tgtgcaactt gaggtgtacc tattgccact
     1081 catgggcgga gggtaaagga aacaccttga ctttcttcaa cttgatgcgg tcaatccacc
     1141 gtttcctttc catacccaat atcaaacgat ttgaatttgt atggcatggc ggagaagtaa
     1201 cacttttgtc cgtcaattat ttcaagaagc taatttggct ccaagagcaa ttcaagaagt
     1261 cggatcaggt catcaccaat tcggttcaga caaatgcggt taatatcccg gaggactggc
     1321 tcgtgttcct taagggaatt ggcatggggg tgggaatctc ggttgacgga atcccagaaa
     1381 tacatgatag tcgacgatta gattaccgcg gaaggccgac atcccataaa gttgccgcag
     1441 gaatgaaaaa gcttaggtcc tacggcatcc cctacggtgc tcttgtagtg gtagacagag
     1501 atgtctatgc ctcgaatatc gagaaaatgc tgtcttactt ttatgaaata ggattaaccg
     1561 acatagagtt tttaaatata gtgccagaca accgttgtca accgggcgat gacccgggtg
     1621 gttcttatat aacttaccac aactatatca actttctgtc aaatgtattc cgggtttggt
     1681 ggcacggcta tcaggacaaa ataaatattc ggctatttca cggcttcata gactctatta
     1741 aaagcagcca gaaaaaaata tctgactgct actgggccgg gaactgcagt caggaaatta
     1801 ttacactcga gcctaacggc acggtgagtg cctgcgataa atacgtcggg gctgagggga
     1861 acaactacgg gagtatcatt gacaacgacc tagggcactt gctcaccaag tctaatacga
     1921 acaaggatca tttaaaagag gaaattgaga gctatgaaaa gatgcatcaa tgcaaatggt
     1981 ttcatctgtg taatggtggg tgtcctcacg accgcgttac gaatagaaag cataatccta
     2041 actataacga tagttgttgt gggacgggtg gtctcttaga aactatcaaa cagacaattg
     2101 cggcttgacc ggtcttgcta gattctaatc aagaggatgt cagaatgcca tttgcctgag
     2161 agatgcaggc ttcatttttg atactttttt atttgtaacc tatatagtat aggatttttt
     2221 ttgtcatttt gtttcttctc gtacgagctt gctcctgatc agcctatctc gcagctgatg
     2281 aatatcttgt ggtaggggtt tgggaaaatc attcgagttt gatgtttttc ttggtatttc
     2341 ccactcctct tcagagtaca gaagattaag tgagaccttc gtttgtgcgg atcccccaca
     2401 caccatagct tcaaaatgtt tctactcctt ttttactctt ccagattttc tcggactccg
     2461 cgcatcgccg taccacttca aaacacccaa gcacagcata ctaaattttc cctctttctt
     2521 cctctagggt gtcgttaatt acccgtacta aaggtttgga aaagaaaaaa gagaccgcct
     2581 cgtttctttt tcttcgtcga aaaaggcaat aaaaattttt atcacgtttc tttttcttga
     2641 aatttttttt tttagttttt ttctctttca gtgacctcca ttgatattta agttaataaa
     2701 cggtcttcaa tttctcaagt ttcagtttca tttttcttgt tctattacaa ctttttttac
     2761 ttcttgttca ttagaaagaa agcatagcaa tctaatctaa gggcggtgtt gacaattaat
     2821 catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca
     2881 agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt cgccggagcg gtcgagttct
     2941 ggaccgaccg gctcgggttc tcccgggact tcgtggagga cgacttcgcc ggtgtggtcc
     3001 gggacgacgt gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc
     3061 tggcctgggt gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt
     3121 ccacgaactt ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg
     3181 ggcgggagtt cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc
     3241 aggactgaca cgtccgacgg cggcccacgg gtcccaggcc tcggagatcc gtcccccttt
     3301 tcctttgtcg atatcatgta attagttatg tcacgcttac attcacgccc tccccccaca
     3361 tccgctctaa ccgaaaagga aggagttaga caacctgaag tctaggtccc tatttatttt
     3421 tttatagtta tgttagtatt aagaacgtta tttatatttc aaatttttct tttttttctg
     3481 tacagacgcg tgtacgcatg taacattata ctgaaaacct tgcttgagaa ggttttggga
     3541 cgctcgaagg ctttaatttg caagctggag accaacatgt gagcaaaagg ccagcaaaag
     3601 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3661 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3721 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3781 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc
     3841 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3901 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3961 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     4021 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     4081 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     4141 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     4201 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     4261 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc atgagatc
//
