LOCUS       .                       7313 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1..693
                     /label="TurboRFP"
                     /note="red fluorescent protein from Entacmaea quadricolor"
     terminator      715..902
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        complement(940..958)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(979..995)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1003..1019)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1027..1057)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1072..1093)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1381..1969)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2143..3000)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(3001..3105)
                     /label="AmpR promoter"
     rep_origin      complement(3132..4474)
                     /label="2μ ori"
                     /note="yeast 2μ plasmid origin of replication"
     promoter        4736..4951
                     /label="URA3 promoter"
     CDS             4952..5752
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(5886..6341)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6482..6498
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6508..6526
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6552..6568
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6613..7279
                     /label="GAP promoter"
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
ORIGIN
        1 atgagcgagc tgatcaagga gaacatgcac atgaagctgt acatggaggg caccgtgaac
       61 aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
      121 atgaagatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctacc
      181 agcttcatgt acggcagcaa agccttcatc aaccacaccc agggcatccc cgacttcttt
      241 aagcagtcct tccctgaggg cttcacatgg gagagaatca ccacatacga agacgggggc
      301 gtgctgaccg ctacccagga caccagcttc cagaacggct gcatcatcta caacgtcaag
      361 atcaacgggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac acgcggctgg
      421 gaggccaaca ccgagatgct gtaccccgct gacggcggcc tgagaggcca cagccagatg
      481 gccctgaagc tcgtgggcgg gggctacctg cactgctcct tcaagaccac atacagatcc
      541 aagaaacccg ctaagaacct caagatgccc ggcttccact tcgtggacca cagactggaa
      601 agaatcaagg aggccgacaa agagacctac gtcgagcagc acgagatggc tgtggccaag
      661 tactgcgacc tccctagcaa actggggcac agataaggat cctagactct cgaggcgaat
      721 ttcttatgat ttatgatttt tattattaaa taagttataa aaaaaataag tgtatacaaa
      781 ttttaaagtg actcttaggt tttaaaacga aaattcttat tcttgagtaa ctctttcctg
      841 taggtcaggt tgctttctca ggtatagcat gaggtcgctc ttattgacca cacctctacc
      901 ggcatgccga gcaccgcggt ggagctccag cttttgttcc ctttagtgag ggttaattgc
      961 gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1021 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1081 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1141 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1201 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1261 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1321 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1381 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1441 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1501 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1561 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1621 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1681 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1741 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     1801 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     1861 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     1921 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     1981 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2041 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2101 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2161 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2221 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2281 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2341 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2401 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2461 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2521 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2581 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2641 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2701 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     2761 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     2821 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     2881 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     2941 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3001 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     3061 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     3121 agtgccacct gaacgaagca tctgtgcttc attttgtaga acaaaaatgc aacgcgagag
     3181 cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa tgcaacgcga
     3241 aagcgctatt ttaccaacga agaatctgtg cttcattttt gtaaaacaaa aatgcaacgc
     3301 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     3361 cgcgagagcg ctattttacc aacaaagaat ctatacttct tttttgttct acaaaaatgc
     3421 atcccgagag cgctattttt ctaacaaagc atcttagatt actttttttc tcctttgtgc
     3481 gctctataat gcagtctctt gataactttt tgcactgtag gtccgttaag gttagaagaa
     3541 ggctactttg gtgtctattt tctcttccat aaaaaaagcc tgactccact tcccgcgttt
     3601 actgattact agcgaagctg cgggtgcatt ttttcaagat aaaggcatcc ccgattatat
     3661 tctataccga tgtggattgc gcatactttg tgaacagaaa gtgatagcgt tgatgattct
     3721 tcattggtca gaaaattatg aacggtttct tctattttgt ctctatatac tacgtatagg
     3781 aaatgtttac attttcgtat tgttttcgat tcactctatg aatagttctt actacaattt
     3841 ttttgtctaa agagtaatac tagagataaa cataaaaaat gtagaggtcg agtttagatg
     3901 caagttcaag gagcgaaagg tggatgggta ggttatatag ggatatagca cagagatata
     3961 tagcaaagag atacttttga gcaatgtttg tggaagcggt attcgcaata ttttagtagc
     4021 tcgttacagt ccggtgcgtt tttggttttt tgaaagtgcg tcttcagagc gcttttggtt
     4081 ttcaaaagcg ctctgaagtt cctatacttt ctagagaata ggaacttcgg aataggaact
     4141 tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac gcgagctgcg cacatacagc
     4201 tcactgttca cgtcgcacct atatctgcgt gttgcctgta tatatatata catgagaaga
     4261 acggcatagt gcgtgtttat gcttaaatgc gtacttatat gcgtctattt atgtaggatg
     4321 aaaggtagtc tagtacctcc tgtgatatta tcccattcca tgcggggtat cgtatgcttc
     4381 cttcagcact accctttagc tgttctatat gctgccactc ctcaattgga ttagtctcat
     4441 ccttcaatgc tatcatttcc tttgatattg gatcatacta agaaaccatt attatcatga
     4501 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
     4561 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
     4621 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     4681 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatacc accttttcaa
     4741 ttcatcattt tttttttatt cttttttttg atttcggttt ccttgaaatt tttttgattc
     4801 ggtaatctcc gaacagaagg aagaacgaag gaaggagcac agacttagat tggtatatat
     4861 acgcatatgt agtgttgaag aaacatgaaa ttgcccagta ttcttaaccc aactgcacag
     4921 aacaaaaacc tgcaggaaac gaagataaat catgtcgaaa gctacatata aggaacgtgc
     4981 tgctactcat cctagtcctg ttgctgccaa gctatttaat atcatgcacg aaaagcaaac
     5041 aaacttgtgt gcttcattgg atgttcgtac caccaaggaa ttactggagt tagttgaagc
     5101 attaggtccc aaaatttgtt tactaaaaac acatgtggat atcttgactg atttttccat
     5161 ggagggcaca gttaagccgc taaaggcatt atccgccaag tacaattttt tactcttcga
     5221 agacagaaaa tttgctgaca ttggtaatac agtcaaattg cagtactctg cgggtgtata
     5281 cagaatagca gaatgggcag acattacgaa tgcacacggt gtggtgggcc caggtattgt
     5341 tagcggtttg aagcaggcgg cagaagaagt aacaaaggaa cctagaggcc ttttgatgtt
     5401 agcagaattg tcatgcaagg gctccctatc tactggagaa tatactaagg gtactgttga
     5461 cattgcgaag agcgacaaag attttgttat cggctttatt gctcaaagag acatgggtgg
     5521 aagagatgaa ggttacgatt ggttgattat gacacccggt gtgggtttag atgacaaggg
     5581 agacgcattg ggtcaacagt atagaaccgt ggatgatgtg gtctctacag gatctgacat
     5641 tattattgtt ggaagaggac tatttgcaaa gggaagggat gctaaggtag agggtgaacg
     5701 ttacagaaaa gcaggctggg aagcatattt gagaagatgc ggccagcaaa actaaaaaac
     5761 tgtattataa gtaaatgcat gtatactaaa ctcacaaatt agagcttcaa tttaattata
     5821 tcagttatta ccctatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     5881 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     5941 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     6001 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     6061 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     6121 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     6181 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     6241 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     6301 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg
     6361 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     6421 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     6481 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attgggtacc
     6541 gggccccccc tcgaggtcga cggtatcgat aagcttgata tcgaattcag tctactagtc
     6601 agttcgagtt tatcattatc aatactgcca tttcaaagaa tacgtaaata attaatagta
     6661 gtgattttcc taactttatt tagtcaaaaa attagccttt taattctgct gtaacccgta
     6721 catgcccaaa atagggggcg ggttacacag aatatataac atcgtaggtg tctgggtgaa
     6781 cagtttattc ctggcatcca ctaaatataa tggagcccgc tttttaagct ggcatccaga
     6841 aaaaaaaaga atcccagcac caaaatattg ttttcttcac caaccatcag ttcataggtc
     6901 cattctctta gcgcaactac agagaacagg ggcacaaaca ggcaaaaaac gggcacaacc
     6961 tcaatggagt gatgcaacct gcctggagta aatgatgaca caaggcaatt gacccacgca
     7021 tgtatctatc tcattttctt acaccttcta ttaccttctg ctctctctga tttggaaaaa
     7081 gctgaaaaaa aaggttgaaa ccagttccct gaaattattc ccctacttga ctaataagta
     7141 tataaagacg gtaggtattg attgtaattc tgtaaatcta tttcttaaac ttcttaaatt
     7201 ctacttttat agttagtctt ttttttagtt ttaaaacacc aagaacttag tttcgaataa
     7261 acacacataa acaaacaaaa gatctgatat ggatcgaatt agatctcgcc acc
//
