LOCUS       .                       6059 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     terminator      complement(13..178)
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        complement(369..768)
                     /label="TEF1 promoter"
                     /note="promoter for EF-1α"
     promoter        complement(785..803)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        823..1466
                     /label="GAP promoter"
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     1472..1488
                     /label="SK primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     complement(1522..1538)
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     terminator      1541..1788
                     /label="CYC1 terminator"
                     /note="transcription terminator for CYC1"
     promoter        1958..2178
                     /label="URA3 promoter"
     CDS             2179..2979
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     CDS             3616..3804
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    3909..4049
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4235..4823)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(4997..5854)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(5855..5959)
                     /label="AmpR promoter"
ORIGIN
        1 gaattgctag cggagcgacc tcatgctata cctgagaaag caacctgacc tacaggaaag
       61 agttactcaa gaataagaat tttcgtttta aaacctaaga gtcactttaa aatttgtata
      121 cacttatttt ttttataact tatttaataa taaaaatcat aaatcataag aaattcgctt
      181 atttagaagt gtcaacaacg tatctaccaa cgatttgacc cttttccatc ttttcgtaaa
      241 tttctggcaa ggtagacaag ccgacaacct tgattggaga cttgaccaaa cctctggcga
      301 agaattgtta attaaagatc tccgcggtcc ggagttaact gatcagcggc cgcgctagtt
      361 ctagaaaact tagattagat tgctatgctt tctttctaat gagcaagaag taaaaaaagt
      421 tgtaatagaa caagaaaaat gaaactgaaa cttgagaaat tgaagaccgt ttattaactt
      481 aaatatcaat gggaggtcat cgaaagagaa aaaaatcaaa aaaaaaattt tcaagaaaaa
      541 gaaacgtgat aaaaattttt attgcctttt tcgacgaaga aaaagaaacg aggcggtctc
      601 ttttttcttt tccaaacctt tagtacgggt aattaacgac accctagagg aagaaagagg
      661 ggaaatttag tatgctgtgc ttgggtgttt tgaagtggta cggcgatgcg cggagtccga
      721 gaaaatctgg aagagtaaaa aaggagtaga aacattttga agctatgagc tccagctttt
      781 gttcccttta gtgagggtta attgcgcgcc gagctcagtt tatcattatc aatactcgcc
      841 atttcaaaga atacgtaaat aattaatagt agtgattttc ctaactttat ttagtcaaaa
      901 aattagcctt ttaattctgc tgtaacccgt acatgcccaa aatagggggc gggttacaca
      961 gaatatataa catcgtaggt gtctgggtga acagtttatt cctggcatcc actaaatata
     1021 atggagcccg ctttttaagc tggcatccag aaaaaaaaag aatcccagca ccaaaatatt
     1081 gttttcttca ccaaccatca gttcataggt ccattctctt agcgcaacta cagagaacag
     1141 gggcacaaac aggcaaaaaa cgggcacaac ctcaatggag tgatgcaacc tgcctggagt
     1201 aaatgatgac acaaggcaat tgacccacgc atgtatctat ctcattttct tacaccttct
     1261 attaccttct gctctctctg atttggaaaa agctgaaaaa aaaggttgaa accagttccc
     1321 tgaaattatt cccctacttg actaataagt atataaagac ggtaggtatt gattgtaatt
     1381 ctgtaaatct atttcttaaa cttcttaaat tctactttta tagttagtct tttttttagt
     1441 tttaaaacac cagaacttag tttcgacgga ttctagaact agtggatccc ccgggctgca
     1501 ggaattcgat atcaagctta tcgataccgt cgacctcgag tcatgtaatt agttatgtca
     1561 cgcttacatt cacgccctcc ccccacatcc gctctaaccg aaaaggaagg agttagacaa
     1621 cctgaagtct aggtccctat ttattttttt atagttatgt tagtattaag aacgttattt
     1681 atatttcaaa tttttctttt ttttctgtac agacgcgtgt acgcatgtaa cattatactg
     1741 aaaaccttgc ttgagaaggt tttgggacgc tcgaaggctt taatttgcgg ccggtacctg
     1801 gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc aatcaattct
     1861 tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc gcgtccgcca
     1921 tctccagcag ccgcacgcgg cgcatccccc cccccctttc aattcaattc atcatttttt
     1981 ttttattctt ttttttgatt tcggtttctt tgaaattttt ttgattcggt aatctccgaa
     2041 cagaaggaag aacgaaggaa ggagcacaga cttagattgg tatatatacg catatgtagt
     2101 gttgaagaaa catgaaattg cccagtattc ttaacccaac tgcacagaac aaaaacctgc
     2161 aggaaacgaa gataaatcat gtcgaaagct acatataagg aacgtgctgc tactcatcct
     2221 agtcctgttg ctgccaagct atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct
     2281 tcattggatg ttcgtaccac caaggaatta ctggagttag ttgaagcatt aggtcccaaa
     2341 atttgtttac taaaaacaca tgtggatatc ttgactgatt tttccatgga gggcacagtt
     2401 aagccgctaa aggcattatc cgccaagtac aattttttac tcttcgaaga cagaaaattt
     2461 gctgacattg gtaatacagt caaattgcag tactctgcgg gtgtatacag aatagcagaa
     2521 tgggcagaca ttacgaatgc acacggtgtg gtgggcccag gtattgttag cggtttgaag
     2581 caggcggcag aagaagtaac aaaggaacct agaggccttt tgatgttagc agaattgtca
     2641 tgcaagggct ccctatctac tggagaatat actaagggta ctgttgacat tgcgaagagc
     2701 gacaaagatt ttgttatcgg ctttattgct caaagagaca tgggtggaag agatgaaggt
     2761 tacgattggt tgattatgac acccggtgtg ggtttagatg acaagggaga cgcattgggt
     2821 caacagtata gaaccgtgga tgatgtggtc tctacaggat ctgacattat tattgttgga
     2881 agaggactat ttgcaaaggg aagggatgct aaggtagagg gtgaacgtta cagaaaagca
     2941 ggctgggaag catatttgag aagatgcggc cagcaaaact aaaaaactgt attataagta
     3001 aatgcatgta tactaaactc acaaattaga gcttcaattt aattatatca gttattaccc
     3061 gggaatctcg gtcgtaatga tttttataat gacgaaaaaa aaaaaattgg aaagaaaagg
     3121 gggggggggc agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag
     3181 gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga
     3241 gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt
     3301 cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat
     3361 gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt
     3421 aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc
     3481 cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat
     3541 cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa attccccctt
     3601 acacggaggc atcaagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc
     3661 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca
     3721 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
     3781 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     3841 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     3901 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
     3961 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     4021 gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc
     4081 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4141 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     4201 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     4261 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     4321 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     4381 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     4441 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     4501 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     4561 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     4621 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag
     4681 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     4741 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     4801 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     4861 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     4921 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     4981 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5041 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5101 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     5161 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     5221 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     5281 atagtttgcg caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg
     5341 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     5401 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     5461 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     5521 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     5581 ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa
     5641 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     5701 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     5761 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     5821 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     5881 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     5941 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca
     6001 ttattatcat gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaa
//
