LOCUS       .                       7337 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     CDS             1..717
                     /label="EYFP"
                     /note="enhanced YFP"
     terminator      739..926
                     /label="ADH1 terminator"
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     promoter        complement(964..982)
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1003..1019)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(1027..1043)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1051..1081)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1096..1117)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1405..1993)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2167..3024)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(3025..3129)
                     /label="AmpR promoter"
     rep_origin      complement(3156..4498)
                     /label="2μ ori"
                     /note="yeast 2μ plasmid origin of replication"
     promoter        4760..4975
                     /label="URA3 promoter"
     CDS             4976..5776
                     /label="URA3"
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     rep_origin      complement(5910..6365)
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     6506..6522
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6532..6550
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     6576..6592
                     /label="KS primer"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        6637..7303
                     /label="GAP promoter"
                     /note="promoter for glyceraldehyde-3-phosphate
                     dehydrogenase; also known as the TDH3 promoter"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccttcggcta cggcctgcag tgcttcgccc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagct accagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa
      721 ggatcctaga ctctcgaggc gaatttctta tgatttatga tttttattat taaataagtt
      781 ataaaaaaaa taagtgtata caaattttaa agtgactctt aggttttaaa acgaaaattc
      841 ttattcttga gtaactcttt cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc
      901 gctcttattg accacacctc taccggcatg ccgagcaccg cggtggagct ccagcttttg
      961 ttccctttag tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt
     1021 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     1081 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     1141 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     1201 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     1261 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     1321 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     1381 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     1441 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     1501 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     1561 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     1621 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     1681 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     1741 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     1801 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     1861 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     1921 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     1981 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     2041 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     2101 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     2161 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     2221 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     2281 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     2341 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     2401 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     2461 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     2521 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     2581 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     2641 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     2701 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     2761 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     2821 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     2881 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     2941 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     3001 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     3061 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     3121 ggttccgcgc acatttcccc gaaaagtgcc acctgaacga agcatctgtg cttcattttg
     3181 tagaacaaaa atgcaacgcg agagcgctaa tttttcaaac aaagaatctg agctgcattt
     3241 ttacagaaca gaaatgcaac gcgaaagcgc tattttacca acgaagaatc tgtgcttcat
     3301 ttttgtaaaa caaaaatgca acgcgagagc gctaattttt caaacaaaga atctgagctg
     3361 catttttaca gaacagaaat gcaacgcgag agcgctattt taccaacaaa gaatctatac
     3421 ttcttttttg ttctacaaaa atgcatcccg agagcgctat ttttctaaca aagcatctta
     3481 gattactttt tttctccttt gtgcgctcta taatgcagtc tcttgataac tttttgcact
     3541 gtaggtccgt taaggttaga agaaggctac tttggtgtct attttctctt ccataaaaaa
     3601 agcctgactc cacttcccgc gtttactgat tactagcgaa gctgcgggtg cattttttca
     3661 agataaaggc atccccgatt atattctata ccgatgtgga ttgcgcatac tttgtgaaca
     3721 gaaagtgata gcgttgatga ttcttcattg gtcagaaaat tatgaacggt ttcttctatt
     3781 ttgtctctat atactacgta taggaaatgt ttacattttc gtattgtttt cgattcactc
     3841 tatgaatagt tcttactaca atttttttgt ctaaagagta atactagaga taaacataaa
     3901 aaatgtagag gtcgagttta gatgcaagtt caaggagcga aaggtggatg ggtaggttat
     3961 atagggatat agcacagaga tatatagcaa agagatactt ttgagcaatg tttgtggaag
     4021 cggtattcgc aatattttag tagctcgtta cagtccggtg cgtttttggt tttttgaaag
     4081 tgcgtcttca gagcgctttt ggttttcaaa agcgctctga agttcctata ctttctagag
     4141 aataggaact tcggaatagg aacttcaaag cgtttccgaa aacgagcgct tccgaaaatg
     4201 caacgcgagc tgcgcacata cagctcactg ttcacgtcgc acctatatct gcgtgttgcc
     4261 tgtatatata tatacatgag aagaacggca tagtgcgtgt ttatgcttaa atgcgtactt
     4321 atatgcgtct atttatgtag gatgaaaggt agtctagtac ctcctgtgat attatcccat
     4381 tccatgcggg gtatcgtatg cttccttcag cactaccctt tagctgttct atatgctgcc
     4441 actcctcaat tggattagtc tcatccttca atgctatcat ttcctttgat attggatcat
     4501 actaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     4561 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     4621 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     4681 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
     4741 gagtgcacca taccaccttt tcaattcatc attttttttt tattcttttt tttgatttcg
     4801 gtttccttga aatttttttg attcggtaat ctccgaacag aaggaagaac gaaggaagga
     4861 gcacagactt agattggtat atatacgcat atgtagtgtt gaagaaacat gaaattgccc
     4921 agtattctta acccaactgc acagaacaaa aacctgcagg aaacgaagat aaatcatgtc
     4981 gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg ccaagctatt
     5041 taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc gtaccaccaa
     5101 ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa aaacacatgt
     5161 ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg cattatccgc
     5221 caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta atacagtcaa
     5281 attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta cgaatgcaca
     5341 cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag aagtaacaaa
     5401 ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc tatctactgg
     5461 agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg ttatcggctt
     5521 tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga ttatgacacc
     5581 cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa ccgtggatga
     5641 tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg caaagggaag
     5701 ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat atttgagaag
     5761 atgcggccag caaaactaaa aaactgtatt ataagtaaat gcatgtatac taaactcaca
     5821 aattagagct tcaatttaat tatatcagtt attaccctat gcggtgtgaa ataccgcaca
     5881 gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt
     5941 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     6001 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     6061 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     6121 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     6181 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     6241 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     6301 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     6361 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     6421 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     6481 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     6541 tcactatagg gcgaattggg taccgggccc cccctcgagg tcgacggtat cgataagctt
     6601 gatatcgaat tcagtctact agtcagttcg agtttatcat tatcaatact gccatttcaa
     6661 agaatacgta aataattaat agtagtgatt ttcctaactt tatttagtca aaaaattagc
     6721 cttttaattc tgctgtaacc cgtacatgcc caaaataggg ggcgggttac acagaatata
     6781 taacatcgta ggtgtctggg tgaacagttt attcctggca tccactaaat ataatggagc
     6841 ccgcttttta agctggcatc cagaaaaaaa aagaatccca gcaccaaaat attgttttct
     6901 tcaccaacca tcagttcata ggtccattct cttagcgcaa ctacagagaa caggggcaca
     6961 aacaggcaaa aaacgggcac aacctcaatg gagtgatgca acctgcctgg agtaaatgat
     7021 gacacaaggc aattgaccca cgcatgtatc tatctcattt tcttacacct tctattacct
     7081 tctgctctct ctgatttgga aaaagctgaa aaaaaaggtt gaaaccagtt ccctgaaatt
     7141 attcccctac ttgactaata agtatataaa gacggtaggt attgattgta attctgtaaa
     7201 tctatttctt aaacttctta aattctactt ttatagttag tctttttttt agttttaaaa
     7261 caccaagaac ttagtttcga ataaacacac ataaacaaac aaaagatctg atatggatcg
     7321 aattagatct cgccacc
//
