LOCUS       .                       6423 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        73..376
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     intron          654..1670
                     /label="chimeric intron"
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
     CDS             1776..3275
                     /label="HIV-1 gag"
                     /note="gag protein from human immunodeficiency virus 1"
     CDS             3276..3302
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             3315..3341
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             3354..3380
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     polyA_signal    3502..3557
                     /label="β-globin poly(A) signal"
                     /note="rabbit β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(3918..3934)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(3942..3958)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3966..3996)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4011..4032)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4090..4286
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    4292..4426
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(4665..5253)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5427..6284)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(6285..6389)
                     /label="AmpR promoter"
ORIGIN
        1 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
       61 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      121 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      181 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      241 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      301 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      361 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
      421 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
      481 ggcggggggg gggggggggc gcgccrgggg gggggggggg gggggggggg gggggggggg
      541 sgrggsggrg rggkgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
      601 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
      661 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
      721 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
      781 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
      841 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
      901 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
      961 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     1021 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1081 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     1141 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     1201 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     1261 cycgggccgg ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc ggcggctgtc
     1321 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1381 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     1441 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1501 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgggccg cgttgacgcg
     1741 cacggcaaga ggcgaggggc ggcgactggt gagagatggg tgcgagagcg tcagtattaa
     1801 gcgggggaga attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca
     2161 gccaaaatta ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac
     2221 ctagaacttt aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga
     2281 tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa
     2341 acacagtggg gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag
     2401 ctgcagaatg ggatagagtg catccagtgc atgcagggcc tattgcacca ggccagatga
     2461 gagaaccaag gggaagtgac atagcaggaa ctactagtac ccttcaggaa caaataggat
     2521 ggatgacaca taatccacct atcccagtag gagaaatcta taaaagatgg ataatcctgg
     2581 gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata agacaaggac
     2641 caaaggaacc ctttagagac tatgtagacc gattctataa aactctaaga gccgagcaag
     2701 cttcacaaga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat gcgaacccag
     2761 attgtaagac tattttaaaa gcattgggac caggagcgac actagaagaa atgatgacag
     2821 catgtcaggg agtgggggga cccggccata aagcaagagt tttggctgaa gcaatgagcc
     2881 aagtaacaaa tccagctacc ataatgatac agaaaggcaa ttttaggaac caaagaaaga
     2941 ctgttaagtg tttcaattgt ggcaaagaag ggcacatagc caaaaattgc agggccccta
     3001 ggaaaaaggg ctgttggaaa tgtggaaagg aaggacacca aatgaaagat tgtactgaga
     3061 gacaggctaa ttttttaggg aagatctggc cttcccacaa gggaaggcca gggaattttc
     3121 ttcagagcag accagagcca acagccccac cagaagagag cttcaggttt ggggaagaga
     3181 caacaactcc ctctcagaag caggagccga tagacaagga actgtatcct ttagcttccc
     3241 tcagatcact ctttggcagc gacccctcgt cacaactccc ccctctggag cgccttactc
     3301 ttggctccct gcagttgccc ccactggagc ggctgaccct ggggtcattg cagttgcctc
     3361 cactcgaacg actgacgttg agccagaact atccgattgt gcagaccggt atgacgcgtg
     3421 cggccgcgct agcagatctt tttccctctg ccaaaaatta tggggacatc atgaagcccc
     3481 ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata gtgtgttgga
     3541 attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta aaacatcaga
     3601 atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc atgaacaaag
     3661 gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc
     3721 atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt
     3781 ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct
     3841 gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac ctgcagccca
     3901 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     3961 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     4021 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     4081 cagcggatcc gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     4141 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4201 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     4261 tttttggagg cctaggcttt tgcaaaaagc taacttgttt attgcagctt ataatggtta
     4321 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4381 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatccgct gcattaatga
     4441 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc
     4501 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg
     4561 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc
     4621 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc
     4681 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga
     4741 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc
     4801 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat
     4861 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg
     4921 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc
     4981 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga
     5041 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact
     5101 agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt
     5161 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
     5221 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
     5281 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa
     5341 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata
     5401 tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg
     5461 atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata
     5521 cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg
     5581 gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct
     5641 gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt
     5701 tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc
     5761 tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga
     5821 tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt
     5881 aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc
     5941 atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa
     6001 tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca
     6061 catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca
     6121 aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct
     6181 tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc
     6241 gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa
     6301 tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt
     6361 tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg
     6421 aca
//
