LOCUS       .                       6796 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        73..376
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     intron          654..1670
                     /label="chimeric intron"
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
     CDS             1776..3275
                     /label="HIV-1 gag"
                     /note="gag protein from human immunodeficiency virus 1"
     CDS             3276..3302
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             3315..3341
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             3354..3380
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     CDS             3411..3797
                     /label="MS2-N55K"
                     /note="bacteriophage MS2 coat protein"
     polyA_signal    3875..3930
                     /label="β-globin poly(A) signal"
                     /note="rabbit β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     primer_bind     complement(4291..4307)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(4315..4331)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-β-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4339..4369)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4384..4405)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4463..4659
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    4665..4799
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(5038..5626)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5800..6657)
                     /label="AmpR"
                     /note="β-lactamase"
     promoter        complement(6658..6762)
                     /label="AmpR promoter"
ORIGIN
        1 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
       61 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      121 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      181 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      241 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      301 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      361 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
      421 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
      481 ggcggggggg gggggggggc gcgccrgggg gggggggggg gggggggggg gggggggggg
      541 sgrggsggrg rggkgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
      601 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
      661 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
      721 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
      781 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
      841 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
      901 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
      961 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     1021 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1081 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     1141 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     1201 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     1261 cycgggccgg ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc ggcggctgtc
     1321 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1381 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     1441 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1501 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgggccg cgttgacgcg
     1741 cacggcaaga ggcgaggggc ggcgactggt gagagatggg tgcgagagcg tcagtattaa
     1801 gcgggggaga attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca
     2161 gccaaaatta ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac
     2221 ctagaacttt aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga
     2281 tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa
     2341 acacagtggg gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag
     2401 ctgcagaatg ggatagagtg catccagtgc atgcagggcc tattgcacca ggccagatga
     2461 gagaaccaag gggaagtgac atagcaggaa ctactagtac ccttcaggaa caaataggat
     2521 ggatgacaca taatccacct atcccagtag gagaaatcta taaaagatgg ataatcctgg
     2581 gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata agacaaggac
     2641 caaaggaacc ctttagagac tatgtagacc gattctataa aactctaaga gccgagcaag
     2701 cttcacaaga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat gcgaacccag
     2761 attgtaagac tattttaaaa gcattgggac caggagcgac actagaagaa atgatgacag
     2821 catgtcaggg agtgggggga cccggccata aagcaagagt tttggctgaa gcaatgagcc
     2881 aagtaacaaa tccagctacc ataatgatac agaaaggcaa ttttaggaac caaagaaaga
     2941 ctgttaagtg tttcaattgt ggcaaagaag ggcacatagc caaaaattgc agggccccta
     3001 ggaaaaaggg ctgttggaaa tgtggaaagg aaggacacca aatgaaagat tgtactgaga
     3061 gacaggctaa ttttttaggg aagatctggc cttcccacaa gggaaggcca gggaattttc
     3121 ttcagagcag accagagcca acagccccac cagaagagag cttcaggttt ggggaagaga
     3181 caacaactcc ctctcagaag caggagccga tagacaagga actgtatcct ttagcttccc
     3241 tcagatcact ctttggcagc gacccctcgt cacaactccc ccctctggag cgccttactc
     3301 ttggctccct gcagttgccc ccactggagc ggctgaccct ggggtcattg cagttgcctc
     3361 cactcgaacg actgacgttg agccagaact atccgattgt gcagaccggt gcttcaaact
     3421 ttactcagtt cgtgctcgtg gacaatggtg ggacagggga tgtgacagtg gctccttcta
     3481 atttcgctaa tggggtggca gagtggatca gctccaactc acggagccag gcctacaagg
     3541 tgacatgcag cgtcaggcag tctagtgccc agaagagaaa gtataccatc aaggtggagg
     3601 tccccaaagt ggctacccag acagtgggcg gagtcgaact gcctgtcgcc gcttggaggt
     3661 cctacctgaa catggagctc actatcccaa ttttcgctac caattctgac tgtgaactca
     3721 tcgtgaaggc aatgcagggg ctcctcaaag acggtaatcc tatcccttcc gccatcgccg
     3781 ctaactcagg tatctactaa gctagcagat ctttttccct ctgccaaaaa ttatggggac
     3841 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     3901 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     3961 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccca tatgctggct
     4021 gccatgaaca aaggttggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc
     4081 cattccttat tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt
     4141 gtgttatttt tttctttaac atccctaaaa ttttccttac atgttttact agccagattt
     4201 ttcctcctct cctgactact cccagtcata gctgtccctc ttctcttatg gagatccctc
     4261 gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     4321 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     4381 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     4441 aaacctgtcg tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta
     4501 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     4561 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     4621 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag
     4681 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4741 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     4801 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4861 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4921 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4981 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5041 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5101 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5161 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5221 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5281 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5341 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5401 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5461 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5521 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5581 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5641 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5701 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5761 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5821 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5881 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5941 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6001 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6061 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6121 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6181 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6241 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6301 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6361 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6421 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6481 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6541 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6601 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6661 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6721 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6781 gccacctggg tcgaca
//
