LOCUS       V012822                 8290 bp    DNA     circular SYN 19-OCT-2023
DEFINITION  Exported.
ACCESSION   V012822
VERSION     V012822
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8290)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..8290
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             98..109
                     /note="strong bacterial ribosome binding site (Elowitz and
                     Leibler, 2000)"
     CDS             206..259
                     /label="S loop"
                     /note="GroES chaperone mobile loop that interacts with
                     GroEL"
     CDS             498..2141
                     /gene="groEL"
                     /label="Chaperonin GroEL"
                     /note="Chaperonin GroEL from Escherichia coli O8 (strain
                     IAI1). Accession#: B7M8Q4"
     CDS             2202..3497
                     /label="Trigger Factor"
                     /note="ribosome-associated chaperone from E. coli (Hoffmann
                     et al., 2010)"
     terminator      4387..4473
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     promoter        complement(4533..4588)
                     /label="tetR/tetA promoters"
                     /note="overlapping promoters for bacterial tetR and tetA"
     CDS             4604..5227
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             complement(6248..6904)
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     promoter        complement(6905..7007)
                     /label="cat promoter"
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     rep_origin      complement(7533..8077)
                     /direction=LEFT
                     /label="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A
                     origin of replication can be propagated in E. coli cells
                     that contain a second plasmid with the ColE1 origin."
ORIGIN
        1 ctcgagaaat cataaaaaat ttatttgctt ccctatcagt gatagagtat aatagagtcg
       61 aattgttagc ggagaagaat ttcacacaga attcattaaa gaggagaaag tcgaggcgtc
      121 acccataaca gatacggact ttctcaaagg agagttatca atgaatattc gtccattgca
      181 tgatcgcgtg atcgtcaagc gtaaagaagt tgaaactaaa tctgctggcg gcatcgttct
      241 gaccggctct gcagcggcta aatccacccg cggcgaagtg ctggctgtcg gcaatggccg
      301 tatccttgaa aatggcgaag tgaagccgct ggatgtgaaa gttggcgaca tcgttatttt
      361 caacgatggc tacggtgtga aatctgagaa gatcgacaat gaagaagtgt tgatcatgtc
      421 cgaaagcgac attctggcaa ttgttgaagc gtaatccgcg cacgacactg aacatacgaa
      481 tttaaggaat aaagataatg gcagctaaag acgtaaaatt cggtaacgac gctcgtgtga
      541 aaatgctgcg cggcgtaaac gtactggcag atgcagtgaa agttaccctc ggtccaaaag
      601 gccgtaacgt agttctggat aaatctttcg gtgcaccgac catcaccaaa gatggtgttt
      661 ccgttgctcg tgaaatcgaa ctggaagaca agttcgaaaa tatgggtgcg cagatggtga
      721 aagaagttgc ctctaaagca aacgacgctg caggcgacgg taccaccact gcaaccgtac
      781 tggctcaggc tatcatcact gaaggtctga aagctgttgc tgcgggcatg aacccgatgg
      841 acctgaaacg tggtatcgac aaagcggtta ccgctgcagt tgaagaactg aaagcgctgt
      901 ccgtaccatg ctctgactct aaagcgattg ctcaggttgg taccatctcc gctaactccg
      961 acgaaaccgt aggtaaactg atcgctgaag cgatggacaa agtcggtaaa gaaggcgtta
     1021 tcaccgttga agacggtacc ggtctgcagg acgaactgga cgtggttgaa ggtatgcagt
     1081 tcgaccgtgg ctacctgtct ccttacttca tcaacaagcc ggaaactggc gcagtagaac
     1141 tggaaagccc gttcatcctg ctggctgaca agaaaatctc caacatccgc gaaatgctgc
     1201 cggttctgga agctgttgcc aaagcaggca aaccgctgct gatcatcgct gaagatgtag
     1261 aaggcgaagc gctggcaact ctggttgtta acaccatgcg tggcatcgtg aaagtcgctg
     1321 cggttaaagc accgggcttc ggcgatcgtc gtaaagctat gctgcaggat atcgcaaccc
     1381 tgactggcgg taccgtgatc tctgaagaga tcggtatgga gctggaaaaa gcaaccctgg
     1441 aagacctggg tcaggctaaa cgtgttgtga tcaacaaaga caccaccact atcatcgatg
     1501 gcgtgggtga agaagctgca atccagggcc gtgttgctca gatccgtcag cagattgaag
     1561 aagcaacttc tgactacgac cgtgaaaaac tgcaggaacg cgtagcgaaa ctggcaggcg
     1621 gcgttgcagt tatcaaagtg ggtgctgcta ccgaagttga aatgaaagag aaaaaagcac
     1681 gcgttgaaga tgccctgcac gcgacccgtg ctgcggtaga agaaggcgtg gttgctggtg
     1741 gtggtgttgc gctgatccgc gtagcgtcta aactggctga cctgcgtggt cagaacgaag
     1801 accagaacgt gggtatcaaa gttgcactgc gtgcaatgga agctccgctg cgtcagatcg
     1861 tattgaactg cggcgaagaa ccgtctgttg ttgctaacac cgttaaaggc ggcgacggca
     1921 actacggtta caacgcagca accgaagaat acggcaacat gatcgacatg ggtatcctgg
     1981 atccaaccaa agtaactcgt tctgctctgc agtacgcagc ttctgtggct ggcctgatga
     2041 tcaccaccga atgcatggtt accgacctgc cgaaaaacga tgcagctgac ttaggcgctg
     2101 ctggcggtat gggcggcatg ggtggcatgg gcggcatgat gtaattgccc tgcacctcgc
     2161 agaaataaac aaacccccct gtgatttttt gaggtaacaa gatgcaagtt tcagttgaaa
     2221 ccactcaagg ccttggccgc cgtgtaacga ttactatcgc tgctgacagc atcgagaccg
     2281 ctgttaaaag cgagctggtc aacgttgcga aaaaagtacg tattgacggc ttccgcaaag
     2341 gcaaagtgcc aatgaatatc gttgctcagc gttatggcgc gtctgtacgc caggacgttc
     2401 tgggtgacct gatgagccgt aacttcattg acgccatcat taaagaaaaa atcaatccgg
     2461 ctggcgcacc gacttatgtt ccgggcgaat acaagctggg tgaagacttc acttactctg
     2521 tagagtttga agtttatccg gaagttgaac tgcagggtct ggaagcgatc gaagttgaaa
     2581 aaccgatcgt tgaagtgacc gacgctgacg ttgacggcat gctggatact ctgcgtaaac
     2641 agcaggcgac ctggaaagaa aaagacggcg ctgttgaagc agaagaccgc gtaaccatcg
     2701 acttcaccgg ttctgtagac ggcgaagagt tcgaaggcgg taaagcgtct gatttcgtac
     2761 tggcgatggg ccagggtcgt atgatcccgg gctttgaaga cggtatcaaa ggccacaaag
     2821 ctggcgaaga gttcaccatc gacgtgacct tcccggaaga ataccacgca gaaaacctga
     2881 aaggtaaagc agcgaaattc gctatcaacc tgaagaaagt tgaagagcgt gaactgccgg
     2941 aactgactgc agaattcatc aaacgtttcg gcgttgaaga tggttccgta gaaggtctgc
     3001 gcgctgaagt gcgtaaaaac atggagcgcg agctgaagag cgccatccgt aaccgcgtta
     3061 agtctcaggc gatcgaaggt ctggtaaaag ctaacgacat cgacgtaccg gctgcgctga
     3121 tcgacagcga aatcgacgtt ctgcgtcgcc aggctgcaca gcgtttcggt ggcaacgaaa
     3181 aacaagctct ggaactgccg cgcgaactgt tcgaagaaca ggctaaacgc cgcgtagttg
     3241 ttggcctgct gctgggcgaa gttatccgca ccaacgagct gaaagctgac gaagagcgcg
     3301 tgaaaggcct gatcgaagag atggcttctg cgtacgaaga tccgaaagaa gttatcgagt
     3361 tctacagcaa aaacaaagaa ctgatggaca acatgcgcaa tgttgctctg gaagaacagg
     3421 ctgttgaagc tgtactggcg aaagcgaaag tgactgaaaa agaaaccact ttcaacgagc
     3481 tgatgaacca gcaggcgtaa tttacgcagc ataacgcgct aaattcgcac aaaggcccgt
     3541 caccgccagg tggtgggctt ttttttgtca tgttaaggaa cgcgtcattt ttctgactgg
     3601 ccaggttgaa gaccacatgg ctaacctgat tgtggcgcag atgctgttcc tggaagcaga
     3661 aaacccagaa aaagatatct atctgtacat taactcccca ggcggggtga tcactgccgg
     3721 gatgtctatc tatgacacca tgcagtttat caagcctgat gtcagcacca tctgtatggg
     3781 ccaggcggcc tcgatgggcg ctttcttgct gaccgcaggg gcaaaaggta aacgtttttg
     3841 cctgccgaat tcgcgcgtga tgattcacca accgttgggc ggctaccagg gccaggcgac
     3901 cgatatcgaa attcatgccc gtgaaattct gaaagttaaa gggcgcatga atgaacttat
     3961 ggcgcttcat acgggtcaat cattagaaca gattgaacgt gataccgagc gcgatcgctt
     4021 cctttccgcc cctgaagcgg tggaatacgg tctggtcgat tcgattctga cccatcgtaa
     4081 ttgatgccag aggcgcaact gtgccgctat acttatccag ggcggcacaa cgctgtaagc
     4141 gcttgcgcct gagaatggca tttgcgtcgt cgtgtgcggc acaaagaaca aagaagaggt
     4201 tttgacccat gacagataaa cgcaaagatg gctcaggcaa attgctgtat tgctcttttt
     4261 gcggcaaaag ccagcatgaa gtgcgcaagc tgattgccgg tccatccgtg tatatctgcg
     4321 acgaatgtgt tgatttatgt aacgacatca ttcggggcag aaatgtctgg gggtttctag
     4381 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     4441 ttgtcggtga acgctctcct gagtaggaca aatccgccgc cctagaccta gggtcgactc
     4501 tagaggatct actagtcata tggatttcac ttttctctat cactgatagg gagtggtaaa
     4561 ataactctat caatgataga gtgtcaacaa aaattaggaa ttaatgatgt ctagattaga
     4621 taaaagtaaa gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt
     4681 aacaacccgt aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt
     4741 aaaaaataag cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac
     4801 tcacttttgc cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag
     4861 ttttagatgt gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc
     4921 tacagaaaaa cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt
     4981 ttcactagag aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt
     5041 attggaagat caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag
     5101 tatgccgcca ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc
     5161 cttcttattc ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag
     5221 tgggtcttaa aagcagcata acctttttcc gtgatggtaa cttcacggta accaagatgt
     5281 cgagttaacc accctttaga ttcataaagc gaaaataatg cggctccaac gtacccacct
     5341 aaatggaaac ggatcggatc tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg
     5401 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
     5461 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
     5521 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtccc
     5581 ctacgtgctg ctgaagttgc ccgcaacaga gagtggaacc aaccggtgat accacgatac
     5641 tatgactgag agtcaacgcc atgagcggcc tcatttctta ttctgagtta caacagtccg
     5701 caccgctgtc cggtagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta
     5761 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgccc aacagtcccc
     5821 cggccacggg gcctgccacc atacccacgc cgaaacaagc gccctgcacc attatgttcc
     5881 ggatctgcat cgcaggatgc tgctggctac cctgtggaac acctacatct gtattaacga
     5941 agcgctaacc gtttttatca ggctctggga ggcagaataa atgatcatat cgtcaattat
     6001 tacctccacg gggagagcct gagcaaactg gcctcaggca tttgagaagc acacggtcac
     6061 actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg ctatttaacg
     6121 accctgccct gaaccgacga ccgggtcgaa tttgctttcg aatttctgcc attcatccgc
     6181 ttattatcac ttattcaggc gtagcaccag gcgtttaagg gcaccaataa ctgccttaaa
     6241 aaaattacgc cccgccctgc cactcatcgc agtactgttg taattcatta agcattctgc
     6301 cgacatggaa gccatcacag acggcatgat gaacctgaat cgccagcggc atcagcacct
     6361 tgtcgccttg cgtataatat ttgcccatgg tgaaaacggg ggcgaagaag ttgtccatat
     6421 tggccacgtt taaatcaaaa ctggtgaaac tcacccaggg attggctgag acgaaaaaca
     6481 tattctcaat aaacccttta gggaaatagg ccaggttttc accgtaacac gccacatctt
     6541 gcgaatatat gtgtagaaac tgccggaaat cgtcgtggta ttcactccag agcgatgaaa
     6601 acgtttcagt ttgctcatgg aaaacggtgt aacaagggtg aacactatcc catatcacca
     6661 gctcaccgtc tttcattgcc atacggaatt ccggatgagc attcatcagg cgggcaagaa
     6721 tgtgaataaa ggccggataa aacttgtgct tatttttctt tacggtcttt aaaaaggccg
     6781 taatatccag ctgaacggtc tggttatagg tacattgagc aactgactga aatgcctcaa
     6841 aatgttcttt acgatgccat tgggatatat caacggtggt atatccagtg atttttttct
     6901 ccattttagc ttccttagct cctgaaaatc tcgataactc aaaaaatacg cccggtagtg
     6961 atcttatttc attatggtga aagttggaac ctcttacgtg ccgatcaacg tctcattttc
     7021 gccaaaagtt ggcccagggc ttcccggtat caacagggac accaggattt atttattctg
     7081 cgaagtgatc ttccgtcaca ggtatttatt cggcgcaaag tgcgtcgggt gatgctgcca
     7141 acttactgat ttagtgtatg atggtgtttt tgaggtgctc cagtggcttc tgtttctatc
     7201 agctgtccct cctgttcagc tactgacggg gtggtgcgta acggcaaaag caccgccgga
     7261 catcagcgct agcggagtgt atactggctt actatgttgg cactgatgag ggtgtcagtg
     7321 aagtgcttca tgtggcagga gaaaaaaggc tgcaccggtg cgtcagcaga atatgtgata
     7381 caggatatat tccgcttcct cgctcactga ctcgctacgc tcggtcgttc gactgcggcg
     7441 agcggaaatg gcttacgaac ggggcggaga tttcctggaa gatgccagga agatacttaa
     7501 cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc ccctgacaag
     7561 catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact ataaagatac
     7621 caggcgtttc ccctggcggc tccctcgtgc gctctcctgt tcctgccttt cggtttaccg
     7681 gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac gcctgacact cagttccggg
     7741 taggcagttc gctccaagct ggactgtatg cacgaacccc ccgttcagtc cgaccgctgc
     7801 gccttatccg gtaactatcg tcttgagtcc aacccggaaa gacatgcaaa agcaccactg
     7861 gcagcagcca ctggtaattg atttagagga gttagtcttg aagtcatgcg ccggttaagg
     7921 ctaaactgaa aggacaagtt ttggtgactg cgctcctcca agccagttac ctcggttcaa
     7981 agagttggta gctcagagaa ccttcgaaaa accgccctgc aaggcggttt tttcgttttc
     8041 agagcaagag attacgcgca gaccaaaacg atctcaagaa gatcatctta ttaatcagat
     8101 aaaatatttc tagatttcag tgcaatttat ctcttcaaat gtagcacctg aagtcagccc
     8161 catacgatat aagttgtaat tctcatgttt gacagcttat catcgataag cttgcatgcc
     8221 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt
     8281 tcgtcttcac
//
